Published customer image: . Sheep antiGreen Fluorescent protein antibody ( 4745-1051 ) used to detect GFP tagged constructs (CG8784:p65) in Drosophila larvae by immunofluorescence. Image caption: . Hugin neurons target neurosecretory cells via peptide-receptor transmission in addition to synaptic connections. A, Promoter-based hugin receptor PK2-R1 driver line CG8784-Gal4::p65 was generated by replacing the first coding exon of the CG8784 loci with GAL4 in a BAC clone containing 80kb flanking genomic context and integrating the final BAC into attP site VK00033. B, CG8784-Gal4::p65 drives expression in cells of the pars intercerebralis (PI). C, Co-staining with Drosophila insulin-like peptide 2 (Dilp2), diuretic hormone 44 (DH44) and Dromyosuppressin (DMS). These peptides are produced by medial neurosecretory cells (mNSCs) in a non-overlapping manner. CG8784-Gal4::p65 drives expression in all mNSCs of the PI. Scale bars in A and B represent 10 &mum. From: Schlegel P, Texada MJ, Miroschnikow A, Schoofs A, H ckesfeld S, Peters M,Schneider-Mizell CM, Lacin H, Li F, Fetter RD, Truman JW, Cardona A, Pankratz MJ. Synaptic transmission parallels neuromodulation in a central food-intake circuit. Elife. 2016 Nov 15;5. pii: e16799 .