小鼠 单克隆 (3D6)
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
Sandwich ELISA analysis of ERK1 binding using Mouse anti Human ERK1 antibody, clone 3C9 as a capture reagent and biotinylated Mouse anti Human ERK1 antibody, clone 3D6 ( MCA4808Z ) as a detection reagent with purified human ERK1 as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader ( Bio-Rad Catalog 168-1135 ). A neat serum sample (green) is indicated
规格: 0.1毫克
价格: 547美元
至产商
小鼠 单克隆
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
domestic rabbit 多克隆
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹
小鼠 单克隆 (F04/4G10)
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
Western Blot analysis of whole cell lysates probed with (A) ERK1/2 antibody ( VMA00485 ) or (B) ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody ( VMA00713 ) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P ). Membranes were treated with (+) and without (-) Lambda Protein Phosphatase as indicated and visualized on the ChemiDoc MP with (A) 40 second or (B)15 second exposure. Arrow points to ERK1 (pThr202)/ERK2 (pTyr204) (molecular weight 42 & 44 kDa).
规格: 100微升
价格: 381美元
至产商