Published customer image: . Mouse anti Human CD140b antibody, clone PR7212 ( 7460-3104 ) used to evaluate PDGFR expression on human muscle-derived stem cells in vitro by flow cytometry. Image caption: . Characterization and phenotype of human mdcs. a. Cultured human mdcs consisted of small, triangular adherent cells and small, round floating cells. Scale bar = 50 um. b. Proliferation curve of one normal human mdc preparation (pN1) and one DMD mdc preparation (pD1). The average mean population doubling time for these cells is 33.79 1.24 hrs. c, d, e, f. Representative images showing the expression of NG2 (c, green), CD49b (d, green), CD29 (e, green) and CD44 (f, green) by pN1 human mdcs. Nuclei were counterstained with 10 ug/ml DAPI (blue). Scale bar = 50 um. g. Representative FACS images showing the expression of PDGFR , ALP, CD49b, CD29, CD71, CD90, Stro-1, CD56 and CD34 by human mdcs. Isotype control is shown in red in each image, and positive signal is shown in green. Note that most of these markers, apart from PDGFR and CD56, were expressed at similar levels among cell preparations. From: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE (2011). Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice. PLoS ONE 6(3): e17454 . This image is from an open access article distributed under the terms of the Creative Commons Attribution License .