Published customer image: . Mouse anti Human CD82 antibody, clone B-L2 used for the identification of CDclusters in cell membrane sheets by immunofluorescence. Image caption: . Physical characteristics of tetraspanin clusters. A: JY B cell membrane sheets were stained for CD37, CD81 or CD82 and imaged by STED microscopy. Right images: magnification of the area indicated in left images. Scale bars represent 5 um in whole sheet images and 0.5 um in zoomed images. B: The diameter of tetraspanin clusters measured from intensity profiles by the full-width at half-maximal intensity (FWHM). At least 120 random clusters were measured derived from at least 3 sheets per condition. Kruskal-Wallis ***. Significance by post hoc analysis is shown (Dunn’s multiple comparison tests between CD53 and other tetraspanins). C: Masks were made for individual CD37 clusters by applying a local threshold on STED images, and of each cluster the circularity was plotted against the area. 405 clusters were analyzed from 3 sheets. Left: dot plot of individual clusters, right: boxplot, Kruskal-Wallis ***, significance by post hoc analysis (Dunn) is shown. D: Density of tetraspanin clusters. For every sheet the analyzed surface area and the number of annotated clusters were determined and the number of clusters per μm2 calculated. At least 7 sheets were analyzed per condition. Kruskal-Wallis ***, significance by post hoc analysis (Dunn) is shown. E: Distances from the center of annotated clusters to the center of their nearest neighbor were determined. The red dashed curves show fits of the nearest neighbor curves with random cluster distributions and the average densities from panel D. Clusters of at least 7 sheets were analyzed per condition. From: Zuidscherwoude M, G ttfert F, Dunlock VM, Figdor CG, van den Bogaart G, van Spriel AB. The tetraspanin web revisited by super-resolution microscopy. Sci Rep. 2015 Jul 17;5:12201.