产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
纯化抗人类CD16
目录 :
302002
规格 :
100微克
价格 :
110美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
3G8
反应物种 :
人类, 小鼠, 猕猴
应用 :
免疫组化, 中和反应, 免疫沉淀, 流式细胞仪, 免疫组化-冰冻切片, 抑制或激活实验, mass cytometry
更多信息或购买 :
文章摘录数: 57
出版应用/物种/样本/稀释参考文献
  • 抑制或激活实验; 人类; 5 ug/ml; 图 s4
Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed 出版商
  • 流式细胞仪; 人类; 图 4b
Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster C, et al. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease. Sci Rep. 2019;9:16941 pubmed 出版商
  • mass cytometry; 人类; 图 2j
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed 出版商
  • 流式细胞仪; 人类; 图 1a
Olin A, Henckel E, Chen Y, Lakshmikanth T, Pou C, Mikes J, et al. Stereotypic Immune System Development in Newborn Children. Cell. 2018;174:1277-1292.e14 pubmed 出版商
  • 流式细胞仪; 猕猴; 图 7a
Kasturi S, Kozlowski P, Nakaya H, Burger M, Russo P, Pham M, et al. Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques. J Virol. 2017;91: pubmed 出版商
  • 流式细胞仪; 人类
Demers K, Makedonas G, Buggert M, Eller M, Ratcliffe S, Goonetilleke N, et al. Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection. PLoS Pathog. 2016;12:e1005805 pubmed 出版商
  • 流式细胞仪; 小鼠; 1:200
Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed 出版商
  • 流式细胞仪; 人类; 图 5
Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby Teglund A, Smeds E, et al. Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo. PLoS Pathog. 2015;11:e1005319 pubmed 出版商
  • 流式细胞仪; 人类; 图 7
Venkatasubramanian S, Tripathi D, Tucker T, Paidipally P, Cheekatla S, Welch E, et al. Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection. Eur J Immunol. 2016;46:464-79 pubmed 出版商
  • 流式细胞仪; 人类; 图 s1b
Schulz A, Mälzer J, Domingo C, Jürchott K, Grützkau A, Babel N, et al. Low Thymic Activity and Dendritic Cell Numbers Are Associated with the Immune Response to Primary Viral Infection in Elderly Humans. J Immunol. 2015;195:4699-711 pubmed 出版商
  • 流式细胞仪; 小鼠
Amos J, Himes J, Armand L, Gurley T, Martinez D, Colvin L, et al. Rapid Development of gp120-Focused Neutralizing B Cell Responses during Acute Simian Immunodeficiency Virus Infection of African Green Monkeys. J Virol. 2015;89:9485-98 pubmed 出版商
  • 流式细胞仪; 人类; 图 s3
Mende N, Kuchen E, Lesche M, Grinenko T, Kokkaliaris K, Hanenberg H, et al. CCND1-CDK4-mediated cell cycle progression provides a competitive advantage for human hematopoietic stem cells in vivo. J Exp Med. 2015;212:1171-83 pubmed 出版商
  • 流式细胞仪; 人类; 图 1
O Brien E, Abdulahad W, Rutgers A, Huitema M, O Reilly V, Coughlan A, et al. Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies. Sci Rep. 2015;5:11888 pubmed 出版商
  • 流式细胞仪; 人类; 图 4
Weldon A, Moldovan I, Cabling M, Hernandez E, Hsu S, Gonzalez J, et al. Surface APRIL Is Elevated on Myeloid Cells and Is Associated with Disease Activity in Patients with Rheumatoid Arthritis. J Rheumatol. 2015;42:749-59 pubmed 出版商
  • 流式细胞仪; 人类; 图 1
Hünniger K, Bieber K, Martin R, Lehnert T, Figge M, Löffler J, et al. A second stimulus required for enhanced antifungal activity of human neutrophils in blood is provided by anaphylatoxin C5a. J Immunol. 2015;194:1199-210 pubmed 出版商
  • 流式细胞仪; 人类; 图 7
Hartung E, Becker M, Bachem A, Reeg N, Jäkel A, Hutloff A, et al. Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. J Immunol. 2015;194:1069-79 pubmed 出版商
  • 流式细胞仪; 人类
Presnell S, Al Attar A, Cichocki F, Miller J, Lutz C. Human natural killer cell microRNA: differential expression of MIR181A1B1 and MIR181A2B2 genes encoding identical mature microRNAs. Genes Immun. 2015;16:89-98 pubmed 出版商
  • 流式细胞仪; 人类
Mandl M, Schmitz S, Weber C, Hristov M. Characterization of the CD14++CD16+ monocyte population in human bone marrow. PLoS ONE. 2014;9:e112140 pubmed 出版商
  • 流式细胞仪; 猕猴
  • 免疫组化; 猕猴
Holder G, McGary C, Johnson E, Zheng R, John V, Sugimoto C, et al. Expression of the mannose receptor CD206 in HIV and SIV encephalitis: a phenotypic switch of brain perivascular macrophages with virus infection. J Neuroimmune Pharmacol. 2014;9:716-26 pubmed 出版商
  • 流式细胞仪; 人类
Wiernik A, Foley B, Zhang B, Verneris M, Warlick E, Gleason M, et al. Targeting natural killer cells to acute myeloid leukemia in vitro with a CD16 x 33 bispecific killer cell engager and ADAM17 inhibition. Clin Cancer Res. 2013;19:3844-55 pubmed 出版商
  • 流式细胞仪; 人类
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed 出版商
Okubo K, Brenner M, Cullere X, Saggu G, Patchen M, Bose N, et al. Inhibitory affinity modulation of FcγRIIA ligand binding by glycosphingolipids by inside-out signaling. Cell Rep. 2021;35:109142 pubmed 出版商
Chevrier S, Zurbuchen Y, Cervia C, Adamo S, Raeber M, de Souza N, et al. A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Rep Med. 2021;2:100166 pubmed 出版商
Korn M, Schmitt H, Angermüller S, Chambers D, Seeling M, Lux U, et al. Siglec-15 on Osteoclasts Is Crucial for Bone Erosion in Serum-Transfer Arthritis. J Immunol. 2020;205:2595-2605 pubmed 出版商
Park J, Kim S, Keam B, Park H, Kim S, Kim M, et al. Anti-tumor effects of NK cells and anti-PD-L1 antibody with antibody-dependent cellular cytotoxicity in PD-L1-positive cancer cell lines. J Immunother Cancer. 2020;8: pubmed 出版商
Lakshmikanth T, Muhammad S, Olin A, Chen Y, Mikes J, Fagerberg L, et al. Human Immune System Variation during 1 Year. Cell Rep. 2020;32:107923 pubmed 出版商
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed 出版商
Steffen U, Koeleman C, Sokolova M, Bang H, Kleyer A, Rech J, et al. IgA subclasses have different effector functions associated with distinct glycosylation profiles. Nat Commun. 2020;11:120 pubmed 出版商
Auladell M, Nguyen T, Garcillán B, Mackay F, Kedzierska K, Fox A. Distinguishing naive- from memory-derived human B cells during acute responses. Clin Transl Immunology. 2019;8:e01090 pubmed 出版商
Dutertre C, Becht E, Irac S, Khalilnezhad A, Narang V, Khalilnezhad S, et al. Single-Cell Analysis of Human Mononuclear Phagocytes Reveals Subset-Defining Markers and Identifies Circulating Inflammatory Dendritic Cells. Immunity. 2019;51:573-589.e8 pubmed 出版商
Ardain A, Domingo Gonzalez R, Das S, Kazer S, Howard N, Singh A, et al. Group 3 innate lymphoid cells mediate early protective immunity against tuberculosis. Nature. 2019;: pubmed 出版商
ELDREDGE L, Creasy R, Presnell S, Debley J, Juul S, Mayock D, et al. Infants with evolving bronchopulmonary dysplasia demonstrate monocyte-specific expression of IL-1 in tracheal aspirates. Am J Physiol Lung Cell Mol Physiol. 2019;317:L49-L56 pubmed 出版商
Li L, Wang Y, Torkelson J, Shankar G, Pattison J, Zhen H, et al. TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment. Cell Stem Cell. 2019;24:271-284.e8 pubmed 出版商
Pohl K, Nichols D, Taylor Cousar J, Saavedra M, Strand M, Nick J, et al. Corticosteroid use and increased CXCR2 levels on leukocytes are associated with lumacaftor/ivacaftor discontinuation in cystic fibrosis patients homozygous for the F508del CFTR mutation. PLoS ONE. 2018;13:e0209026 pubmed 出版商
Jog N, Chakravarty E, Guthridge J, James J. Epstein Barr Virus Interleukin 10 Suppresses Anti-inflammatory Phenotype in Human Monocytes. Front Immunol. 2018;9:2198 pubmed 出版商
Ravindran A, Rönnberg E, Dahlin J, Mazzurana L, Säfholm J, Orre A, et al. An Optimized Protocol for the Isolation and Functional Analysis of Human Lung Mast Cells. Front Immunol. 2018;9:2193 pubmed 出版商
Sung J, Patel S, Clohosey M, Roesch L, Tripic T, Kuruc J, et al. HIV-Specific, Ex Vivo Expanded T Cell Therapy: Feasibility, Safety, and Efficacy in ART-Suppressed HIV-Infected Individuals. Mol Ther. 2018;26:2496-2506 pubmed 出版商
Sibener L, Fernandes R, Kolawole E, Carbone C, Liu F, McAffee D, et al. Isolation of a Structural Mechanism for Uncoupling T Cell Receptor Signaling from Peptide-MHC Binding. Cell. 2018;174:672-687.e27 pubmed 出版商
Boutboul D, Kuehn H, Van de Wyngaert Z, Niemela J, Callebaut I, Stoddard J, et al. Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency. J Clin Invest. 2018;128:3071-3087 pubmed 出版商
Frobel J, Rahmig S, Franzen J, Waskow C, Wagner W. Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice. Clin Epigenetics. 2018;10:67 pubmed 出版商
Zhong Q, Gong F, Gong Z, Hua S, Zeng K, Gao X. IgG Immunocomplexes Sensitize Human Monocytes for Inflammatory Hyperactivity via Transcriptomic and Epigenetic Reprogramming in Rheumatoid Arthritis. J Immunol. 2018;200:3913-3925 pubmed 出版商
Chevrier S, Crowell H, Zanotelli V, Engler S, Robinson M, Bodenmiller B. Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry. Cell Syst. 2018;6:612-620.e5 pubmed 出版商
Cao Q, Wang Y, Wang C, Wang X, Lee V, Zheng G, et al. Therapeutic potential of regulatory macrophages generated from peritoneal dialysate in adriamycin nephropathy. Am J Physiol Renal Physiol. 2018;314:F561-F571 pubmed 出版商
Miyamoto D, Lee R, Kalinich M, LiCausi J, Zheng Y, Chen T, et al. An RNA-Based Digital Circulating Tumor Cell Signature Is Predictive of Drug Response and Early Dissemination in Prostate Cancer. Cancer Discov. 2018;8:288-303 pubmed 出版商
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Winkler M, Rissiek A, Priefler M, Schwedhelm E, Robbe L, Bauer A, et al. Human leucocyte antigen (HLA-DR) gene expression is reduced in sepsis and correlates with impaired TNF? response: A diagnostic tool for immunosuppression?. PLoS ONE. 2017;12:e0182427 pubmed 出版商
Prodjinotho U, von Horn C, Debrah A, Batsa Debrah L, Albers A, Layland L, et al. Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes. PLoS Negl Trop Dis. 2017;11:e0005777 pubmed 出版商
Fasbender F, Claus M, Wingert S, Sandusky M, Watzl C. Differential Requirements for Src-Family Kinases in SYK or ZAP70-Mediated SLP-76 Phosphorylation in Lymphocytes. Front Immunol. 2017;8:789 pubmed 出版商
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产品信息
抗原 :
CD16
Apps. Abbrev. :
Flow Cytometry, CyTOF®, Block, IHC-F, IP, Stim
目录 :
302002
克隆 :
3G8
产品 :
纯化抗人类CD16
抗体亚型 :
Mouse IgG1, κ
其他名称 :
FcγRIII, Fc丙受体, Fc丙受体3
规格 :
100微克
价格(美元) :
110美元
反应物种 :
Human, Cynomolgus, Rhesus
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
PURE
免疫原 :
人类PMN细胞
应用注释 :
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。