产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
纯化抗人类HLA-A, B, C
目录 :
311402
规格 :
100微克
价格 :
89美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
W6/32
反应物种 :
人类, 小鼠, 猕猴
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 中和反应, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片, 抑制或激活实验, 其他, mass cytometry
更多信息或购买 :
文章摘录数: 71
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 图 s1
Poletto E, Colella P, Pimentel Vera L, Khan S, Tomatsu S, Baldo G, et al. Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. Mol Ther Methods Clin Dev. 2022;25:392-409 pubmed 出版商
  • mass cytometry; 人类; 图 5b
van der Heide V, Jangra S, Cohen P, Rathnasinghe R, Aslam S, Aydillo T, et al. Limited extent and consequences of pancreatic SARS-CoV-2 infection. Cell Rep. 2022;38:110508 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100; 图 6
Han E, Qian H, Jiang B, Figetakis M, Kosyakova N, Tellides G, et al. A therapeutic vascular conduit to support in vivo cell-secreted therapy. NPJ Regen Med. 2021;6:40 pubmed 出版商
  • 抑制或激活实验; 人类; 5 ug/ml; 图 2e
  • 流式细胞仪; 人类; 图 2b
Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed 出版商
  • 酶联免疫吸附测定; 人类
Hsiue E, Wright K, Douglass J, Hwang M, Mog B, Pearlman A, et al. Targeting a neoantigen derived from a common TP53 mutation. Science. 2021;371: pubmed 出版商
  • 流式细胞仪; 人类; 1:75; 图 2b
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed 出版商
  • 流式细胞仪; 人类
Merola J, Reschke M, Pierce R, Qin L, Spindler S, Baltazar T, et al. Progenitor-derived human endothelial cells evade alloimmunity by CRISPR/Cas9-mediated complete ablation of MHC expression. JCI Insight. 2019;4: pubmed 出版商
  • 流式细胞仪; 人类; 图 s8
Pavel Dinu M, Wiebking V, Dejene B, Srifa W, Mantri S, Nicolas C, et al. Gene correction for SCID-X1 in long-term hematopoietic stem cells. Nat Commun. 2019;10:1634 pubmed 出版商
  • 流式细胞仪; 人类; 图 s1
Liu R, Merola J, Manes T, Qin L, Tietjen G, Lopez Giraldez F, et al. Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1. JCI Insight. 2018;3: pubmed 出版商
  • 流式细胞仪; 人类; 1:50; 图 2a
Barkal A, Weiskopf K, Kao K, Gordon S, Rosental B, Yiu Y, et al. Engagement of MHC class I by the inhibitory receptor LILRB1 suppresses macrophages and is a target of cancer immunotherapy. Nat Immunol. 2018;19:76-84 pubmed 出版商
  • 流式细胞仪; 人类; 图 5b
Mwimanzi F, Toyoda M, Mahiti M, Mann J, Martin J, Bangsberg D, et al. Resistance of Major Histocompatibility Complex Class B (MHC-B) to Nef-Mediated Downregulation Relative to that of MHC-A Is Conserved among Primate Lentiviruses and Influences Antiviral T Cell Responses in HIV-1-Infected Individuals. J Virol. 2018;92: pubmed 出版商
  • 流式细胞仪; 人类; 图 s1b
Burr M, Sparbier C, Chan Y, Williamson J, Woods K, Beavis P, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017;549:101-105 pubmed 出版商
  • 抑制或激活实验; 人类
Zhang X, Lian X, Dai Z, Zheng H, Chen X, Zheng Y. ?3-Deletion Isoform of HLA-A11 Modulates Cytotoxicity of NK Cells: Correlations with HIV-1 Infection of Cells. J Immunol. 2017;199:2030-2042 pubmed 出版商
  • 免疫细胞化学; 人类; 1:500; 图 1a
Johnson D, Wayt J, Wilson J, Donaldson J. Arf6 and Rab22 mediate T cell conjugate formation by regulating clathrin-independent endosomal membrane trafficking. J Cell Sci. 2017;130:2405-2415 pubmed 出版商
  • 流式细胞仪; 人类; 图 e6a
Dever D, Bak R, Reinisch A, Camarena J, Washington G, Nicolas C, et al. CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cells. Nature. 2016;539:384-389 pubmed 出版商
  • 流式细胞仪; 人类; 图 3e, 3f
Albanese M, Tagawa T, Bouvet M, Maliqi L, Lutter D, Hoser J, et al. Epstein-Barr virus microRNAs reduce immune surveillance by virus-specific CD8+ T cells. Proc Natl Acad Sci U S A. 2016;113:E6467-E6475 pubmed
  • 流式细胞仪; 人类
Tagawa T, Albanese M, Bouvet M, Moosmann A, Mautner J, Heissmeyer V, et al. Epstein-Barr viral miRNAs inhibit antiviral CD4+ T cell responses targeting IL-12 and peptide processing. J Exp Med. 2016;213:2065-80 pubmed 出版商
  • 流式细胞仪; 人类; 图 1a
Sadallah S, Schmied L, Eken C, Charoudeh H, Amicarella F, Schifferli J. Platelet-Derived Ectosomes Reduce NK Cell Function. J Immunol. 2016;197:1663-71 pubmed 出版商
  • 流式细胞仪; 人类; 图 s2
Liu C, LeClair P, Monajemi M, Sly L, Reid G, Lim C. α-Integrin expression and function modulates presentation of cell surface calreticulin. Cell Death Dis. 2016;7:e2268 pubmed 出版商
  • 流式细胞仪; 人类; 图 3c
Reches A, Nachmani D, Berhani O, Duev Cohen A, Shreibman D, Ophir Y, et al. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins. J Immunol. 2016;196:4967-76 pubmed 出版商
  • 流式细胞仪; 人类; 表 1
Li H, Borrego F, Nagata S, Tolnay M. Fc Receptor-like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue-like Memory B Cells. J Immunol. 2016;196:4064-74 pubmed 出版商
  • 流式细胞仪; 人类; 图 s6b
Srivastava P, Paluch B, Matsuzaki J, James S, Collamat Lai G, Blagitko Dorfs N, et al. Induction of cancer testis antigen expression in circulating acute myeloid leukemia blasts following hypomethylating agent monotherapy. Oncotarget. 2016;7:12840-56 pubmed 出版商
  • 流式细胞仪; 人类; 1:100; 图 2
Johnson D, Estrada M, Salgado R, Sanchez V, Doxie D, Opalenik S, et al. Melanoma-specific MHC-II expression represents a tumour-autonomous phenotype and predicts response to anti-PD-1/PD-L1 therapy. Nat Commun. 2016;7:10582 pubmed 出版商
  • 免疫组化; 人类; 1:50
Yawata N, Selva K, Liu Y, Tan K, Lee A, Siak J, et al. Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection. Mucosal Immunol. 2016;9:159-70 pubmed 出版商
  • 其他; 人类; 图 2
Jørgensen M, Bæk R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles. 2015;4:26048 pubmed 出版商
  • 流式细胞仪; 人类; 表 1
Boerman G, van Ostaijen Ten Dam M, Kraal K, Santos S, Ball L, Lankester A, et al. Role of NKG2D, DNAM-1 and natural cytotoxicity receptors in cytotoxicity toward rhabdomyosarcoma cell lines mediated by resting and IL-15-activated human natural killer cells. Cancer Immunol Immunother. 2015;64:573-83 pubmed 出版商
  • 流式细胞仪; 人类; 图 1
  • 免疫细胞化学; 人类; 图 3
Bradley S, Chen Z, Melendez B, Talukder A, Khalili J, Rodríguez Cruz T, et al. BRAFV600E Co-opts a Conserved MHC Class I Internalization Pathway to Diminish Antigen Presentation and CD8+ T-cell Recognition of Melanoma. Cancer Immunol Res. 2015;3:602-9 pubmed 出版商
  • 酶联免疫吸附测定; 人类; 图 s1
Nambiar J, Clarke A, Shim D, Mabon D, Tian C, Windloch K, et al. Potent neutralizing anti-CD1d antibody reduces lung cytokine release in primate asthma model. MAbs. 2015;7:638-50 pubmed 出版商
  • 抑制或激活实验; 人类; 15 ug/ml; 图 4
Tomimaru Y, Mishra S, Safran H, Charpentier K, Martin W, De Groot A, et al. Aspartate-β-hydroxylase induces epitope-specific T cell responses in hepatocellular carcinoma. Vaccine. 2015;33:1256-66 pubmed 出版商
  • 流式细胞仪; 人类; 图 1
Boltjes A, van Montfoort N, Biesta P, Op den Brouw M, Kwekkeboom J, van der Laan L, et al. Kupffer cells interact with hepatitis B surface antigen in vivo and in vitro, leading to proinflammatory cytokine production and natural killer cell function. J Infect Dis. 2015;211:1268-78 pubmed 出版商
  • 免疫组化; 小鼠; 1:50; 图 3
Jung Y, Wang J, Lee E, McGee S, Berry J, Yumoto K, et al. Annexin 2-CXCL12 interactions regulate metastatic cell targeting and growth in the bone marrow. Mol Cancer Res. 2015;13:197-207 pubmed 出版商
  • 免疫印迹; 人类; 图 2
Kang W, Sung P, Park S, Yoon S, Chang D, Kim S, et al. Hepatitis C virus attenuates interferon-induced major histocompatibility complex class I expression and decreases CD8+ T cell effector functions. Gastroenterology. 2014;146:1351-60.e1-4 pubmed 出版商
  • 流式细胞仪; 人类; 图 2d
Svajger U, Obermajer N, Jeras M. IFN-?-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. J Leukoc Biol. 2014;95:33-46 pubmed 出版商
Ganan Gomez I, Yang H, Ma F, Montalban Bravo G, Thongon N, Marchica V, et al. Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy. Nat Med. 2022;28:557-567 pubmed 出版商
Pettmann J, Huhn A, Abu Shah E, Kutuzov M, Wilson D, Dustin M, et al. The discriminatory power of the T cell receptor. elife. 2021;10: pubmed 出版商
Willimsky G, Beier C, Immisch L, Papafotiou G, Scheuplein V, Goede A, et al. In vitro proteasome processing of neo-splicetopes does not predict their presentation in vivo. elife. 2021;10: pubmed 出版商
Ruiz Cuevas M, Hardy M, Hollý J, Bonneil E, Durette C, Courcelles M, et al. Most non-canonical proteins uniquely populate the proteome or immunopeptidome. Cell Rep. 2021;34:108815 pubmed 出版商
Tanaka Y, Wada H, Goto R, Osada T, Yamamura K, Fukaya S, et al. TAS0314, a novel multi-epitope long peptide vaccine, showed synergistic antitumor immunity with PD-1/PD-L1 blockade in HLA-A*2402 mice. Sci Rep. 2020;10:17284 pubmed 出版商
Pierini S, Tanyi J, Simpkins F, George E, Uribe Herranz M, Drapkin R, et al. Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target. JCI Insight. 2020;5: pubmed 出版商
Dinh H, Eggert T, Meyer M, Zhu Y, Olingy C, Llewellyn R, et al. Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone Marrow. Immunity. 2020;53:319-334.e6 pubmed 出版商
Rennier K, Shin W, Krug E, Virdi G, Pachynski R. Chemerin Reactivates PTEN and Suppresses PD-L1 in Tumor Cells via Modulation of a Novel CMKLR1-mediated Signaling Cascade. Clin Cancer Res. 2020;26:5019-5035 pubmed 出版商
Cohen M, Zhang S, Sengupta S, Ma H, Bell G, Horton B, et al. Formation of Human Neuroblastoma in Mouse-Human Neural Crest Chimeras. Cell Stem Cell. 2020;26:579-592.e6 pubmed 出版商
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed 出版商
Martin J, Chang C, Boschetti G, Ungaro R, Giri M, Grout J, et al. Single-Cell Analysis of Crohn's Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy. Cell. 2019;178:1493-1508.e20 pubmed 出版商
Makarkov A, Golizeh M, Ruiz Lancheros E, Gopal A, Costas Cancelas I, Chierzi S, et al. Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages. NPJ Vaccines. 2019;4:17 pubmed 出版商
Carlsten M, Namazi A, Reger R, Levy E, Berg M, St Hilaire C, et al. Bortezomib sensitizes multiple myeloma to NK cells via ER-stress-induced suppression of HLA-E and upregulation of DR5. Oncoimmunology. 2019;8:e1534664 pubmed 出版商
Abdul Salam V, Russomanno G, Chien Nien C, Mahomed A, Yates L, Wilkins M, et al. CLIC4/Arf6 Pathway. Circ Res. 2019;124:52-65 pubmed 出版商
Fu J, Zuber J, Martinez M, Shonts B, Obradovic A, Wang H, et al. Human Intestinal Allografts Contain Functional Hematopoietic Stem and Progenitor Cells that Are Maintained by a Circulating Pool. Cell Stem Cell. 2019;24:227-239.e8 pubmed 出版商
Wroblewska A, Dhainaut M, Ben Zvi B, Rose S, Park E, Amir E, et al. Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens. Cell. 2018;175:1141-1155.e16 pubmed 出版商
Garrido C, Clohosey M, Whitworth C, Hudgens M, Margolis D, Soriano Sarabia N. γδ T cells: an immunotherapeutic approach for HIV cure strategies. JCI Insight. 2018;3: pubmed 出版商
Fitzgerald W, Gomez Lopez N, Erez O, Romero R, Margolis L. Extracellular vesicles generated by placental tissues ex vivo: A transport system for immune mediators and growth factors. Am J Reprod Immunol. 2018;80:e12860 pubmed 出版商
Chevrier S, Crowell H, Zanotelli V, Engler S, Robinson M, Bodenmiller B. Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry. Cell Syst. 2018;6:612-620.e5 pubmed 出版商
Chen W, Wang C, Lu C, Chen C, Lee H, Hung S, et al. The Function of HLA-B*13:01 Involved in the Pathomechanism of Dapsone-Induced Severe Cutaneous Adverse Reactions. J Invest Dermatol. 2018;138:1546-1554 pubmed 出版商
Jasmer B, Muschol Steinmetz C, Kreis N, Friemel A, Kielland Kaisen U, Brüggmann D, et al. Involvement of the oncogene B-cell lymphoma 6 in the fusion and differentiation process of trophoblastic cells of the placenta. Oncotarget. 2017;8:108643-108654 pubmed 出版商
Andersen R, Westergaard M, Kjeldsen J, Muller A, Pedersen N, Hadrup S, et al. T-cell Responses in the Microenvironment of Primary Renal Cell Carcinoma-Implications for Adoptive Cell Therapy. Cancer Immunol Res. 2018;6:222-235 pubmed 出版商
Tsuchiya N, Hosono A, Yoshikawa T, Shoda K, Nosaka K, Shimomura M, et al. Phase I study of glypican-3-derived peptide vaccine therapy for patients with refractory pediatric solid tumors. Oncoimmunology. 2017;7:e1377872 pubmed 出版商
Blees A, Januliene D, Hofmann T, Koller N, Schmidt C, Trowitzsch S, et al. Structure of the human MHC-I peptide-loading complex. Nature. 2017;551:525-528 pubmed 出版商
Mirandola L, Pedretti E, Figueroa J, Chiaramonte R, Colombo M, Chapman C, et al. Cancer testis antigen Sperm Protein 17 as a new target for triple negative breast cancer immunotherapy. Oncotarget. 2017;8:74378-74390 pubmed 出版商
Mujib S, Saiyed A, Fadel S, Bozorgzad A, Aidarus N, Yue F, et al. Pharmacologic HIV-1 Nef blockade promotes CD8 T cell-mediated elimination of latently HIV-1-infected cells in vitro. JCI Insight. 2017;2: pubmed 出版商
Lavin Y, Kobayashi S, Leader A, Amir E, Elefant N, Bigenwald C, et al. Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses. Cell. 2017;169:750-765.e17 pubmed 出版商
Chen H, Joalland N, Bridgeman J, Alchami F, Jarry U, Khan M, et al. Synergistic targeting of breast cancer stem-like cells by human γδ T cells and CD8+ T cells. Immunol Cell Biol. 2017;95:620-629 pubmed 出版商
Nöll A, Thomas C, Herbring V, Zollmann T, Barth K, Mehdipour A, et al. Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP. Proc Natl Acad Sci U S A. 2017;114:E438-E447 pubmed 出版商
Liu Y, Burness M, Martin Trevino R, Guy J, Bai S, Harouaka R, et al. RAD51 Mediates Resistance of Cancer Stem Cells to PARP Inhibition in Triple-Negative Breast Cancer. Clin Cancer Res. 2017;23:514-522 pubmed 出版商
Yumoto K, Eber M, Wang J, Cackowski F, Decker A, Lee E, et al. Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow. Sci Rep. 2016;6:36520 pubmed 出版商
Ansari A, Lee Montiel F, Amos J, Imoukhuede P. Secondary anchor targeted cell release. Biotechnol Bioeng. 2015;112:2214-27 pubmed 出版商
Crivello P, Lauterbach N, Zito L, Sizzano F, Toffalori C, Marcon J, et al. Effects of transmembrane region variability on cell surface expression and allorecognition of HLA-DP3. Hum Immunol. 2013;74:970-7 pubmed 出版商
Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M. Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010;2:139-51 pubmed 出版商
Laing B, Ross D, Meyer S, Campbell P, Halpin A, West L, et al. Glutaraldehyde treatment of allograft tissue decreases allosensitization after the Norwood procedure. J Thorac Cardiovasc Surg. 2010;139:1402-8 pubmed 出版商
Lünemann A, Lunemann J, Roberts S, Messmer B, Barreira da Silva R, Raine C, et al. Human NK cells kill resting but not activated microglia via NKG2D- and NKp46-mediated recognition. J Immunol. 2008;181:6170-7 pubmed
McLoughlin R, Lee J, Kasper D, Tzianabos A. IFN-gamma regulated chemokine production determines the outcome of Staphylococcus aureus infection. J Immunol. 2008;181:1323-32 pubmed
Takahara M, Miyai M, Tomiyama M, Mutou M, Nicol A, Nieda M. Copulsing tumor antigen-pulsed dendritic cells with zoledronate efficiently enhance the expansion of tumor antigen-specific CD8+ T cells via Vgamma9gammadelta T cell activation. J Leukoc Biol. 2008;83:742-54 pubmed
产品信息
抗原 :
HLA-A
Apps. Abbrev. :
流式细胞仪, 活性的, 中和, 免疫组化-F, 免疫沉淀, 西方墨点法
目录 :
311402
克隆 :
W6/32
产品 :
纯化抗人类HLA-A, B, C
抗体亚型 :
Mouse IgG2a, κ
其他名称 :
Major Histocompatibility Class I, MHC class I
规格 :
100微克
价格(美元) :
89美元
反应物种 :
Human, Cynomolgus, Rhesus
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
PURE
应用注释 :
Clone W6/32 recognizes residues in the N terminus of the human 2-microglobulin molecule 21 . Additional reported applications (for the relevant formats) include: immunoprecipitaton 2 , Western blotting (non-reducing) 3 , immunohistochemical staining of acetone-fixed frozen tissue sections 4,5 , blocking 6,7 , inhibition of NK cell-mediated lysis 10 , and activation 8,9 . Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections 17 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。