产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
Ultra-LEAF™ Purified anti-human CD3
目录 :
317325
规格 :
100微克
价格 :
99美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
鼠单克隆抗体-CD3
反应物种 :
人类, 小鼠
应用 :
流式细胞仪, 免疫组化-石蜡切片, 抑制或激活实验, 其他
更多信息或购买 :
文章摘录数: 65
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 图 2b
Hickman T, Choi E, Whiteman K, Muralidharan S, Pai T, Johnson T, et al. BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity. PLoS ONE. 2022;17:e0266980 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 s1a
Wang Z, He L, Li W, Xu C, Zhang J, Wang D, et al. GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma. J Immunother Cancer. 2021;9: pubmed 出版商
  • 流式细胞仪; 人类
Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed 出版商
  • 流式细胞仪; 人类; 图 5a, 5c
Harro C, Perez Sanz J, Costich T, Payne K, Anadon C, Chaurio R, et al. Methyltransferase inhibitors restore SATB1 protective activity against cutaneous T cell lymphoma in mice. J Clin Invest. 2021;131: pubmed 出版商
  • 其他; 小鼠
Tseng H, Xiong W, Badeti S, Yang Y, Ma M, Liu T, et al. Efficacy of anti-CD147 chimeric antigen receptors targeting hepatocellular carcinoma. Nat Commun. 2020;11:4810 pubmed 出版商
  • 流式细胞仪; 人类; 图 s3a
Fischer M, Ruhnau J, Schulze J, Obst D, Floel A, Vogelgesang A. Spermine and spermidine modulate T-cell function in older adults with and without cognitive decline ex vivo. Aging (Albany NY). 2020;12:13716-13739 pubmed 出版商
  • 流式细胞仪; 人类; 图 1a
Pecher A, Kettemann F, Asteriti E, Schmid H, Duerr Stoerzer S, Keppeler H, et al. Invariant natural killer T cells are functionally impaired in patients with systemic sclerosis. Arthritis Res Ther. 2019;21:212 pubmed 出版商
  • 流式细胞仪; 人类; 图 1a
Lim S, Kim J, Jeon S, Shin M, Kwon J, Kim T, et al. Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients. Front Immunol. 2019;10:496 pubmed 出版商
  • 抑制或激活实验; 人类; 5 ug/ml; 图 s10e
Lodygin D, Hermann M, Schweingruber N, Flügel Koch C, Watanabe T, Schlosser C, et al. β-Synuclein-reactive T cells induce autoimmune CNS grey matter degeneration. Nature. 2019;566:503-508 pubmed 出版商
  • 流式细胞仪; 人类; 图 s1a
Muller Durovic B, Grählert J, Devine O, Akbar A, Hess C. CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age. Aging (Albany NY). 2019;11:724-740 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 s14a
Tan H, Jegaskanda S, Juno J, Esterbauer R, Wong J, Kelly H, et al. Subdominance and poor intrinsic immunogenicity limit humoral immunity targeting influenza HA stem. J Clin Invest. 2019;129:850-862 pubmed 出版商
  • 流式细胞仪; 人类; 图 s3c
Wiedemann G, Aithal C, Kraechan A, Heise C, Cadilha B, Zhang J, et al. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma. Transl Oncol. 2019;12:350-360 pubmed 出版商
  • 抑制或激活实验; 人类; 1 ug/ml; 图 8f
Klement J, Paschall A, Redd P, Ibrahim M, Lu C, Yang D, et al. An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion. J Clin Invest. 2018;128:5549-5560 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 图 6d
Jung I, Kim Y, Yu H, Lee M, Kim S, Lee J. CRISPR/Cas9-Mediated Knockout of DGK Improves Antitumor Activities of Human T Cells. Cancer Res. 2018;78:4692-4703 pubmed 出版商
  • 抑制或激活实验; 人类; 图 6a
Yang C, Chiu L, Chang C, Chuang H, Tan T. Induction of DUSP14 ubiquitination by PRMT5-mediated arginine methylation. FASEB J. 2018;:fj201800244RR pubmed 出版商
  • 流式细胞仪; 人类; 图 s4a
Yeo L, Woodwyk A, Sood S, Lorenc A, Eichmann M, Pujol Autonell I, et al. Autoreactive T effector memory differentiation mirrors β cell function in type 1 diabetes. J Clin Invest. 2018;128:3460-3474 pubmed 出版商
  • 流式细胞仪; 人类; 图 2a
Kiani Z, Dupuy F, Bruneau J, Lebouché B, Zhang C, Jackson E, et al. HLA-F on HLA-Null 721.221 Cells Activates Primary NK Cells Expressing the Activating Killer Ig-like Receptor KIR3DS1. J Immunol. 2018;201:113-123 pubmed 出版商
  • 流式细胞仪; 人类; 图 1a
Provine N, Binder B, FitzPatrick M, Schuch A, Garner L, Williamson K, et al. Unique and Common Features of Innate-Like Human Vδ2+ γδT Cells and Mucosal-Associated Invariant T Cells. Front Immunol. 2018;9:756 pubmed 出版商
  • 流式细胞仪; 人类; 图 4a
Jackson E, Zhang C, Kiani Z, Lisovsky I, Tallon B, Del Corpo A, et al. HIV exposed seronegative (HESN) compared to HIV infected individuals have higher frequencies of telomeric Killer Immunoglobulin-like Receptor (KIR) B motifs; Contribution of KIR B motif encoded genes to NK cell responsiveness. PLoS ONE. 2017;12:e0185160 pubmed 出版商
  • 流式细胞仪; 人类
Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, et al. IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition. Cancer Res. 2017;77:3268-3279 pubmed 出版商
  • 流式细胞仪; 人类; 图 3d
Jeffery H, Jeffery L, Lutz P, Corrigan M, Webb G, Hirschfield G, et al. Low-dose interleukin-2 promotes STAT-5 phosphorylation, Treg survival and CTLA-4-dependent function in autoimmune liver diseases. Clin Exp Immunol. 2017;188:394-411 pubmed 出版商
  • 流式细胞仪; 人类; 图 s1a
Martin Gayo E, Cronin J, Hickman T, Ouyang Z, Lindqvist M, Kolb K, et al. Circulating CXCR5+CXCR3+PD-1lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth. JCI Insight. 2017;2:e89574 pubmed 出版商
  • 流式细胞仪; 人类; 图 s3a
Aagaard K, Lahon A, Suter M, Arya R, Seferovic M, Vogt M, et al. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication. Sci Rep. 2017;7:41389 pubmed 出版商
  • 抑制或激活实验; 人类; 图 2a
  • 流式细胞仪; 人类; 图 2a
Ribeiro S, Tesio M, Ribot J, Macintyre E, Barata J, Silva Santos B. Casein kinase 2 controls the survival of normal thymic and leukemic ?? T cells via promotion of AKT signaling. Leukemia. 2017;31:1603-1610 pubmed 出版商
  • 流式细胞仪; 人类; 图 4b
Ye C, Wang W, Cheng L, Li G, Wen M, Wang Q, et al. Glycosylphosphatidylinositol-Anchored Anti-HIV scFv Efficiently Protects CD4 T Cells from HIV-1 Infection and Deletion in hu-PBL Mice. J Virol. 2017;91: pubmed 出版商
  • 抑制或激活实验; 人类; 图 s4a
Serr I, Fürst R, Ott V, Scherm M, Nikolaev A, Gökmen F, et al. miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6659-E6668 pubmed
  • 流式细胞仪; 人类; 图 s1
Isitman G, Tremblay McLean A, Lisovsky I, Bruneau J, Lebouché B, Routy J, et al. NK Cells Expressing the Inhibitory Killer Immunoglobulin-Like Receptors (iKIR) KIR2DL1, KIR2DL3 and KIR3DL1 Are Less Likely to Be CD16+ than Their iKIR Negative Counterparts. PLoS ONE. 2016;11:e0164517 pubmed 出版商
  • 抑制或激活实验; 人类
Jung Y, Riven I, Feigelson S, Kartvelishvily E, Tohya K, Miyasaka M, et al. Three-dimensional localization of T-cell receptors in relation to microvilli using a combination of superresolution microscopies. Proc Natl Acad Sci U S A. 2016;113:E5916-E5924 pubmed
  • 流式细胞仪; 人类; 图 5a
Wahid R, Fresnay S, Levine M, Sztein M. Cross-reactive multifunctional CD4+ T cell responses against Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B in humans following immunization with live oral typhoid vaccine Ty21a. Clin Immunol. 2016;173:87-95 pubmed 出版商
  • 流式细胞仪; 人类; 图 s6a
Landtwing V, Raykova A, Pezzino G, Beziat V, Marcenaro E, Graf C, et al. Cognate HLA absence in trans diminishes human NK cell education. J Clin Invest. 2016;126:3772-3782 pubmed 出版商
  • 流式细胞仪; 人类; 图 2
Theorell J, Bryceson Y. Analysis of Intracellular Ca(2+) Mobilization in Human NK Cell Subsets by Flow Cytometry. Methods Mol Biol. 2016;1441:117-30 pubmed 出版商
  • 流式细胞仪; 人类; 图 1a
Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed 出版商
  • 流式细胞仪; 小鼠
Akhmetzyanova I, Drabczyk M, Neff C, Gibbert K, Dietze K, Werner T, et al. PD-L1 Expression on Retrovirus-Infected Cells Mediates Immune Escape from CD8+ T Cell Killing. PLoS Pathog. 2015;11:e1005224 pubmed 出版商
  • 流式细胞仪; 人类; 图 4
Cichocki F, Cooley S, Davis Z, DeFor T, Schlums H, Zhang B, et al. CD56dimCD57+NKG2C+ NK cell expansion is associated with reduced leukemia relapse after reduced intensity HCT. Leukemia. 2016;30:456-63 pubmed 出版商
  • 流式细胞仪; 人类; 图 2
Leeansyah E, Svärd J, Dias J, Buggert M, Nyström J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed 出版商
  • 流式细胞仪; 人类; 1:200; 图 s3
Adoro S, Cubillos Ruiz J, Chen X, Deruaz M, Vrbanac V, Song M, et al. IL-21 induces antiviral microRNA-29 in CD4 T cells to limit HIV-1 infection. Nat Commun. 2015;6:7562 pubmed 出版商
  • 流式细胞仪; 人类; 1:200
Lee J, Breton G, Aljoufi A, Zhou Y, PUHR S, Nussenzweig M, et al. Clonal analysis of human dendritic cell progenitor using a stromal cell culture. J Immunol Methods. 2015;425:21-6 pubmed 出版商
  • 抑制或激活实验; 人类; 图 s7
Campia I, Buondonno I, Castella B, Rolando B, Kopecka J, Gazzano E, et al. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells. PLoS ONE. 2015;10:e0126159 pubmed 出版商
  • 流式细胞仪; 人类; 图 4
Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed 出版商
  • 流式细胞仪; 人类
Willmann K, Klaver S, DoÄŸu F, Santos Valente E, Garncarz W, Bilic I, et al. Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity. Nat Commun. 2014;5:5360 pubmed 出版商
  • 抑制或激活实验; 人类
Boyd Tressler A, Penuela S, Laird D, Dubyak G. Chemotherapeutic drugs induce ATP release via caspase-gated pannexin-1 channels and a caspase/pannexin-1-independent mechanism. J Biol Chem. 2014;289:27246-63 pubmed 出版商
  • 流式细胞仪; 人类
Prinz P, Mendler A, Brech D, Masouris I, Oberneder R, Noessner E. NK-cell dysfunction in human renal carcinoma reveals diacylglycerol kinase as key regulator and target for therapeutic intervention. Int J Cancer. 2014;135:1832-41 pubmed 出版商
Babin L, Darchen A, Robert E, Aid Z, Borry R, Soudais C, et al. De novo generation of the NPM-ALK fusion recapitulates the pleiotropic phenotypes of ALK+ ALCL pathogenesis and reveals the ROR2 receptor as target for tumor cells. Mol Cancer. 2022;21:65 pubmed 出版商
Glassman C, Mathiharan Y, Jude K, Su L, Panova O, Lupardus P, et al. Structural basis for IL-12 and IL-23 receptor sharing reveals a gateway for shaping actions on T versus NK cells. Cell. 2021;184:983-999.e24 pubmed 出版商
Clayton K, Mylvaganam G, Villasmil Ocando A, Stuart H, Maus M, Rashidian M, et al. HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell Host Microbe. 2021;29:435-447.e9 pubmed 出版商
Saragovi A, Abramovich I, Omar I, Arbib E, Toker O, Gottlieb E, et al. Systemic hypoxia inhibits T cell response by limiting mitobiogenesis via matrix substrate-level phosphorylation arrest. elife. 2020;9: pubmed 出版商
Hu B, Tang T, Jia J, Xie B, Wu T, Sheng Y, et al. CD137 agonist induces gastric cancer cell apoptosis by enhancing the functions of CD8+ T cells via NF-κB signaling. Cancer Cell Int. 2020;20:513 pubmed 出版商
Sekine T, Perez Potti A, Rivera Ballesteros O, Stralin K, Gorin J, Olsson A, et al. Robust T Cell Immunity in Convalescent Individuals with Asymptomatic or Mild COVID-19. Cell. 2020;183:158-168.e14 pubmed 出版商
Campos Carrascosa L, van Beek A, de Ruiter V, Doukas M, Wei J, Fisher T, et al. FcγRIIB engagement drives agonistic activity of Fc-engineered αOX40 antibody to stimulate human tumor-infiltrating T cells. J Immunother Cancer. 2020;8: pubmed 出版商
Pan W, Sharabi A, Ferretti A, Zhang Y, Burbano C, Yoshida N, et al. PPP2R2D suppresses IL-2 production and Treg function. JCI Insight. 2020;5: pubmed 出版商
Si J, Shi X, Sun S, Zou B, Li Y, An D, et al. Hematopoietic Progenitor Kinase1 (HPK1) Mediates T Cell Dysfunction and Is a Druggable Target for T Cell-Based Immunotherapies. Cancer Cell. 2020;38:551-566.e11 pubmed 出版商
Evgin L, Huff A, Wongthida P, Thompson J, Kottke T, Tonne J, et al. Oncolytic virus-derived type I interferon restricts CAR T cell therapy. Nat Commun. 2020;11:3187 pubmed 出版商
Correnti C, Hallinan J, Doyle L, Ruff R, Jaeger Ruckstuhl C, Xu Y, et al. Engineering and functionalization of large circular tandem repeat protein nanoparticles. Nat Struct Mol Biol. 2020;27:342-350 pubmed 出版商
Mancuso R, Casper J, Schmidt A, Krahenbuhl S, Weitz Schmidt G. Anti-αLβ2 antibodies reveal novel endocytotic cross-modulatory functionality. Br J Pharmacol. 2020;177:2696-2711 pubmed 出版商
Grandclaudon M, Perrot Dockès M, Trichot C, Karpf L, Abouzid O, Chauvin C, et al. A Quantitative Multivariate Model of Human Dendritic Cell-T Helper Cell Communication. Cell. 2019;179:432-447.e21 pubmed 出版商
Sun T, Ju M, Dai X, Dong H, Gu W, Gao Y, et al. Multilevel defects in the hematopoietic niche in essential thrombocythemia. Haematologica. 2020;105:661-673 pubmed 出版商
Wang J, RIEDER S, Wu J, Hayes S, Halpin R, de los Reyes M, et al. Evaluation of ultra-low input RNA sequencing for the study of human T cell transcriptome. Sci Rep. 2019;9:8445 pubmed 出版商
Garris C, Arlauckas S, Kohler R, Trefny M, Garren S, Piot C, et al. Successful Anti-PD-1 Cancer Immunotherapy Requires T Cell-Dendritic Cell Crosstalk Involving the Cytokines IFN-γ and IL-12. Immunity. 2018;49:1148-1161.e7 pubmed 出版商
Bak R, Dever D, Porteus M. CRISPR/Cas9 genome editing in human hematopoietic stem cells. Nat Protoc. 2018;13:358-376 pubmed 出版商
Hu Y, Kim J, He K, Wan Q, Kim J, Flach M, et al. Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion. J Exp Med. 2016;213:2759-2772 pubmed
Biggs M, Milone M, Santos L, Gondarenko A, Wind S. High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates. J R Soc Interface. 2011;8:1462-71 pubmed 出版商
Pinto J, Dias V, Zoller H, Porto G, Carmo H, Carvalho F, et al. Hepcidin messenger RNA expression in human lymphocytes. Immunology. 2010;130:217-30 pubmed 出版商
Manevich Mendelson E, Feigelson S, Pasvolsky R, Aker M, Grabovsky V, Shulman Z, et al. Loss of Kindlin-3 in LAD-III eliminates LFA-1 but not VLA-4 adhesiveness developed under shear flow conditions. Blood. 2009;114:2344-53 pubmed 出版商
Alter G, Rihn S, Streeck H, Teigen N, Piechocka Trocha A, Moss K, et al. Ligand-independent exhaustion of killer immunoglobulin-like receptor-positive CD8+ T cells in human immunodeficiency virus type 1 infection. J Virol. 2008;82:9668-77 pubmed 出版商
Jeong H, Lee Y, Seo S, Lee S, Park S, Lee J, et al. Blocking of monocyte-associated B7-H1 (CD274) enhances HCV-specific T cell immunity in chronic hepatitis C infection. J Leukoc Biol. 2008;83:755-64 pubmed
产品信息
抗原 :
CD3
Apps. Abbrev. :
流式细胞仪
目录 :
317325
克隆 :
鼠单克隆抗体-CD3
产品 :
Ultra-LEAF™ Purified anti-human CD3
抗体亚型 :
Mouse IgG2a, κ
其他名称 :
T3, CD3ε
规格 :
100微克
价格(美元) :
99美元
反应物种 :
人类
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
ULEAF
应用注释 :
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. Clone OKT3 can block the binding of clones SK7 and UCHT1. 4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。