产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
True-Nuclear™ Mouse Treg Flow Kit
目录 :
320029
规格 :
25试验
价格 :
528美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
AF488
克隆名称 :
150D
反应物种 :
人类, 小鼠, 大鼠, 猕猴
应用 :
免疫印迹, 流式细胞仪, 染色质免疫沉淀
更多信息或购买 :
BioLegend 产品网页
文章摘录数: 28
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 大鼠; 图 10a
Bath N, Verhoven B, Wilson N, Zeng W, Zhong W, Coons L, et al. APRIL/BLyS deficient rats prevent donor specific antibody (DSA) production and cell proliferation in rodent kidney transplant model. PLoS ONE. 2022;17:e0275564 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 s5e
Lee A, Pingali S, Pinilla Ibarz J, Atchison M, Koumenis C, Argon Y, et al. Loss of AID exacerbates the malignant progression of CLL. Leukemia. 2022;36:2430-2442 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 2f
Wu B, Song M, Dong Q, Xiang G, Li J, Ma X, et al. UBR5 promotes tumor immune evasion through enhancing IFN-γ-induced PDL1 transcription in triple negative breast cancer. Theranostics. 2022;12:5086-5102 pubmed 出版商
  • 流式细胞仪; 人类; 图 1c
Pan C, Wu Q, Wang S, Mei Z, Zhang L, Gao X, et al. Combination with Toll-like receptor 4 (TLR4) agonist reverses GITR agonism mediated M2 polarization of macrophage in Hepatocellular carcinoma. Oncoimmunology. 2022;11:2073010 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 5g
Horiuchi S, Wu H, Liu W, Schmitt N, Provot J, Liu Y, et al. Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells. Sci Adv. 2021;7:eabj1249 pubmed 出版商
  • 流式细胞仪; 小鼠; 1:100; 图 1a
Amoozgar Z, Kloepper J, Ren J, Tay R, Kazer S, Kiner E, et al. Targeting Treg cells with GITR activation alleviates resistance to immunotherapy in murine glioblastomas. Nat Commun. 2021;12:2582 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 3b
Cornelissen L, Blanas A, van der Horst J, Kruijssen L, Zaal A, O Toole T, et al. Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis. Int J Cancer. 2019;144:2290-2302 pubmed 出版商
  • 流式细胞仪; 猕猴; 图 2g
Hoang T, Harper J, Pino M, Wang H, Micci L, King C, et al. Bone Marrow-Derived CD4+ T Cells Are Depleted in Simian Immunodeficiency Virus-Infected Macaques and Contribute to the Size of the Replication-Competent Reservoir. J Virol. 2019;93: pubmed 出版商
  • 流式细胞仪; 小鼠; 图 s2a
Varelias A, Bunting M, Ormerod K, Koyama M, Olver S, Straube J, et al. Recipient mucosal-associated invariant T cells control GVHD within the colon. J Clin Invest. 2018;128:1919-1936 pubmed 出版商
  • 流式细胞仪; 小鼠; 1:50; 图 5b
Hasan Z, Koizumi S, Sasaki D, Yamada H, Arakaki N, Fujihara Y, et al. JunB is essential for IL-23-dependent pathogenicity of Th17 cells. Nat Commun. 2017;8:15628 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 4c
Yanagita T, Murata Y, Tanaka D, Motegi S, Arai E, Daniwijaya E, et al. Anti-SIRPα antibodies as a potential new tool for cancer immunotherapy. JCI Insight. 2017;2:e89140 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠; 图 4
Apostolidis S, Rodríguez Rodríguez N, Suárez Fueyo A, Dioufa N, Ozcan E, Crispín J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 5d
Seifert L, Werba G, Tiwari S, Giao Ly N, Nguy S, Alothman S, et al. Radiation Therapy Induces Macrophages to Suppress T-Cell Responses Against Pancreatic Tumors in Mice. Gastroenterology. 2016;150:1659-1672.e5 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 6
Tagliamonte M, Petrizzo A, Napolitano M, Luciano A, Rea D, Barbieri A, et al. A novel multi-drug metronomic chemotherapy significantly delays tumor growth in mice. J Transl Med. 2016;14:58 pubmed 出版商
  • 免疫印迹; 小鼠; 1:500; 图 4
Castiglioni A, Corna G, Rigamonti E, Basso V, Vezzoli M, Monno A, et al. FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration. PLoS ONE. 2015;10:e0128094 pubmed 出版商
  • 流式细胞仪; 人类; 图 5
Romani R, Pirisinu I, Calvitti M, Pallotta M, Gargaro M, Bistoni G, et al. Stem cells from human amniotic fluid exert immunoregulatory function via secreted indoleamine 2,3-dioxygenase1. J Cell Mol Med. 2015;19:1593-605 pubmed 出版商
  • 流式细胞仪; 大鼠; 图 7
Xie L, Choudhury G, Winters A, Yang S, Jin K. Cerebral regulatory T cells restrain microglia/macrophage-mediated inflammatory responses via IL-10. Eur J Immunol. 2015;45:180-91 pubmed 出版商
Du Y, Peng Q, Cheng D, Pan T, Sun W, Wang H, et al. Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells. Nat Commun. 2022;13:231 pubmed 出版商
Wragg K, Tan H, Kristensen A, Nguyen Robertson C, Kelleher A, Parsons M, et al. High CD26 and Low CD94 Expression Identifies an IL-23 Responsive Vδ2+ T Cell Subset with a MAIT Cell-like Transcriptional Profile. Cell Rep. 2020;31:107773 pubmed 出版商
Kataru R, Ly C, Shin J, Park H, Baik J, Rehal S, et al. Tumor lymphatic function regulates tumor inflammatory and immunosuppressive microenvironments. Cancer Immunol Res. 2019;: pubmed 出版商
Liu Y, Xing X, Wang J, Xing L, Su Y, Yao Z, et al. Sterigmatocystin alters the number of FoxP3+ regulatory T cells and plasmacytoid dendritic cells in BALB/c mice. Food Chem Toxicol. 2012;50:1920-6 pubmed 出版商
Haque A, Stanley A, Amante F, Rivera F, Zhou Y, Kuns R, et al. Therapeutic glucocorticoid-induced TNF receptor-mediated amplification of CD4+ T cell responses enhances antiparasitic immunity. J Immunol. 2010;184:2583-92 pubmed 出版商
Klunker S, Chong M, Mantel P, Palomares O, Bassin C, Ziegler M, et al. Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells. J Exp Med. 2009;206:2701-15 pubmed 出版商
Fallarino F, Luca G, Calvitti M, Mancuso F, Nastruzzi C, Fioretti M, et al. Therapy of experimental type 1 diabetes by isolated Sertoli cell xenografts alone. J Exp Med. 2009;206:2511-26 pubmed 出版商
Golovina T, Mikheeva T, Suhoski M, Aqui N, Tai V, Shan X, et al. CD28 costimulation is essential for human T regulatory expansion and function. J Immunol. 2008;181:2855-68 pubmed
Jordan J, Woods M, Olano J, Walker D. The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses. Infect Immun. 2008;76:3717-24 pubmed 出版商
Valencia X, Yarboro C, Illei G, Lipsky P. Deficient CD4+CD25high T regulatory cell function in patients with active systemic lupus erythematosus. J Immunol. 2007;178:2579-88 pubmed
Mayack S, Berg L. Cutting edge: an alternative pathway of CD4+ T cell differentiation is induced following activation in the absence of gamma-chain-dependent cytokine signals. J Immunol. 2006;176:2059-63 pubmed
产品信息
抗原 :
FOXP3
Apps. Abbrev. :
流式细胞仪细胞内染色
目录 :
320029
克隆 :
150D
产品 :
True-Nuclear™ Mouse Treg Flow Kit
其他名称 :
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
规格 :
25试验
价格(美元) :
528美元
反应物种 :
小鼠
克隆性 :
单克隆
共轭标签/标签/标记 :
套
应用注释 :
Materials Provided: 1. Alexa Fluor 488 anti-mouse FOXP3 - 25 tests 2. Alexa Fluor 488 Mouse IgG1, ? isotype control - 25 tests 3. True-Nuclear Transcription Factor Buffer Set - 120 tests (Cat. No. 424401 ) 4. anti-mouse CD4 APC/CD25 PE Cocktail - 50 tests Materials Not Included: 1. Cell Staining Buffer (Cat. No. 420201) 2. Single color compensation controls . Immunofluorescence Staining Procedures: 1. Perform cell surface staining as described in BioLegend s Cell Surface Immunofluorescence Staining Protocol . Add 20 uL of the anti-mouse CD4 APC/CD25 PE cocktail to each tube and incubate in the dark for 20 minutes. 2. Add 2 mL of the cell staining buffer, centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 3. Repeat Step 2, for a total of two washes. 4. Add 1 mL of the Transcription Factor 1X Fix solution to each tube, vortex, and incubate at room temperature in the dark for 45-60 minutes. 5. Without washing, add 2 mL of the Transcription Factor 1X Perm Buffer to each tube. 6. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 7. Add 2 mL of the Transcription Factor 1X Perm Buffer to each tube. 8. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 9. Resuspend the cell pellet in 100 ul of the Transcription Factor 1XPerm Buffer. 10. Add 5 uL of Alexa Fluor 488 anti-mouse FOXP3 antibody or 5 uL of Alexa Fluor 488 mouse IgG1, ? isotype control into the appropriate tubes. Incubate in the dark at room temperature for at least 30 minutes. 11. Add 2 mL of the Transcription Factor 1X Perm Buffer to each tube. 12. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 13. Add 2 mL of the cell staining buffer. 14. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 15. Resuspend in 0.5 mL cell staining buffer and then acquire tubes on a flow cytometer. Caution : The True-Nuclear Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. NOTE: For flow cytometric staining with this clone, True-Nuclear Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3/Perm Buffer Set (Cat. No. 421403).
更多信息或购买 :
BioLegend 产品网页
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品，旨在加速生命科学领域的研发工作。