True-Nuclear™ Human Treg Flow™ Kit | BioLegend 320403 产品信息

产品简要

公司名称 :

BioLegend

其他品牌 :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

产品类型 :

抗体

产品名称 :

True-Nuclear™ Human Treg Flow™ Kit

320403

规格 :

25试验

价格 :

528美元

克隆性 :

单克隆

宿主 :

小鼠

共轭标签 :

AF488

克隆名称 :

259D

反应物种 :

人类

应用 :

流式细胞仪

更多信息或购买 :

BioLegend 产品网页

文章摘录数: 15

出版应用/物种/样本/稀释	参考文献
流式细胞仪; 人类; 图 s8c	Wen Z, Jin K, Shen Y, Yang Z, Li Y, Wu B, et al. N-myristoyltransferase deficiency impairs activation of kinase AMPK and promotes synovial tissue inflammation. Nat Immunol. 2019;20:313-325 pubmed 出版商
流式细胞仪; 人类; 图 5a	Richardson J, Armbruster N, Günter M, Henes J, Autenrieth S. Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells. Front Immunol. 2018;9:2603 pubmed 出版商
	Hassan A, Kafai N, Dmitriev I, Fox J, Smith B, Harvey I, et al. A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2. Cell. 2020;183:169-184.e13 pubmed 出版商
	Ge C, Monk I, Pizzolla A, Wang N, Bedford J, Stinear T, et al. Bystander Activation of Pulmonary Trm Cells Attenuates the Severity of Bacterial Pneumonia by Enhancing Neutrophil Recruitment. Cell Rep. 2019;29:4236-4244.e3 pubmed 出版商
	Dupont G, Demaret J, Venet F, Malergue F, Malcus C, Poitevin Later F, et al. Comparative dose-responses of recombinant human IL-2 and IL-7 on STAT5 phosphorylation in CD4+FOXP3- cells versus regulatory T cells: a whole blood perspective. Cytokine. 2014;69:146-9 pubmed 出版商
	Vudattu N, Waldron Lynch F, Truman L, Deng S, Preston Hurlburt P, Torres R, et al. Humanized mice as a model for aberrant responses in human T cell immunotherapy. J Immunol. 2014;193:587-96 pubmed 出版商
	Ferraro A, D Alise A, Raj T, Asinovski N, Phillips R, Ergun A, et al. Interindividual variation in human T regulatory cells. Proc Natl Acad Sci U S A. 2014;111:E1111-20 pubmed 出版商
	Long S, Cerosaletti K, Bollyky P, Tatum M, Shilling H, Zhang S, et al. Defects in IL-2R signaling contribute to diminished maintenance of FOXP3 expression in CD4(+)CD25(+) regulatory T-cells of type 1 diabetic subjects. Diabetes. 2010;59:407-15 pubmed 出版商
	Long S, Walker M, Rieck M, James E, Kwok W, Sanda S, et al. Functional islet-specific Treg can be generated from CD4+CD25- T cells of healthy and type 1 diabetic subjects. Eur J Immunol. 2009;39:612-20 pubmed 出版商
	Gong G, Shao L, Wang Y, Chen C, Huang D, Yao S, et al. Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection. Blood. 2009;113:837-45 pubmed 出版商
	Long S, Buckner J. Combination of rapamycin and IL-2 increases de novo induction of human CD4(+)CD25(+)FOXP3(+) T cells. J Autoimmun. 2008;30:293-302 pubmed 出版商
	Tran D, Ramsey H, Shevach E. Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype. Blood. 2007;110:2983-90 pubmed
	Groh V, Smythe K, Dai Z, Spies T. Fas-ligand-mediated paracrine T cell regulation by the receptor NKG2D in tumor immunity. Nat Immunol. 2006;7:755-62 pubmed
	Gavin M, Torgerson T, Houston E, deRoos P, Ho W, Stray Pedersen A, et al. Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006;103:6659-64 pubmed
	Yang Z, Novak A, Stenson M, Witzig T, Ansell S. Intratumoral CD4+CD25+ regulatory T-cell-mediated suppression of infiltrating CD4+ T cells in B-cell non-Hodgkin lymphoma. Blood. 2006;107:3639-46 pubmed

产品信息

抗原 :

FOXP3

Apps. Abbrev. :

流式细胞仪细胞内染色

320403

克隆 :

259D

产品 :

True-Nuclear™ Human Treg Flow™ Kit

其他名称 :

Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2

规格 :

25试验

价格(美元) :

528美元

反应物种 :

人类

克隆性 :

单克隆

共轭标签/标签/标记 :

套

免疫原 :

全长FOXP3蛋白

应用注释 :

Materials Provided: 1. Alexa Fluor 488 anti-human FOXP3 - 25 tests 2. Alexa Fluor 488 Mouse IgG1, isotype control - 25 tests 3. True-Nuclear Transcription Factor Buffer Set - 120 tests (Cat. No. 424401 ) 4. anti-human CD4 PE-Cy5/CD25 PE Cocktail - 50 tests Materials Not Included: 1. Cell Staining Buffer (Cat. No. 420201 ) 2. Single color compensation controls Immunofluorescence Staining Procedures: 1. Perform cell surface staining as described in BioLegend s Cell Surface Immunofluorescence Staining Protocol . Add 20 uL of the anti-human CD4 PE-Cy5/CD25 PE cocktail to each tube and incubate in the dark for 20 minutes. 2. Add 2 mL of the cell staining buffer, centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 3. Repeat Step 2, for a total of two washes. 4. Add 1 mL of the Transcription Factor 1X Fix solution to each tube, vortex, and incubate at room temperature in the dark for 45-60 minutes. 5. Without washing, add 2 mL of the Transcription Factor 1X Perm Buffer to each tube. 6. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 7. Add 2 mL of the Transcription Factor 1X Perm Buffer to each tube. 8. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 9. Resuspend the cell pellet in 100 uL of the Transcription Factor 1XPerm Buffer. 10. Add 5 uL of Alexa Fluor 488 anti-human FOXP3 antibody or 5 uL of Alexa Fluor 488 mouse IgG1, isotype control into the appropriate tubes. Incubate in the dark at room temperature for at least 30 minutes. 11. Add 2 mL of the Transcription Factor 1X Perm Buffer to each tube. 12. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 13. Add 2 mL of the cell staining buffer. 14. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 15. Resuspend in 0.5 mL cell staining buffer and then acquire tubes on a flow cytometer. Caution : The True-Nuclear Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. NOTE: For flow cytometric staining with this clone, True-Nuclear Transcription Factor Buffer Set (Cat. No. 424401 ) offers improved staining and is highly recommended.

更多信息或购买 :

BioLegend 产品网页

公司信息

BioLegend

8999 BioLegend Way
San Diego, CA 92121

customerserv@biolegend.com

https://www.biolegend.com

1-858-455-9588

公司总部: 美国

Biolegend公司为生物医学研究提供顶级的抗体及试剂产品，旨在加速生命科学领域的研发工作。