产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
纯化抗人类Ki67抗原
目录 :
350501
规格 :
25微克
价格 :
55美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
Ki67抗原
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 19
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 1:20; 图 3a
Huang Y, Huang J, Huang Y, Gan L, Long L, Pu A, et al. TFRC promotes epithelial ovarian cancer cell proliferation and metastasis via up-regulation of AXIN2 expression. Am J Cancer Res. 2020;10:131-147 pubmed
  • 流式细胞仪; 人类; 图 1b
Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 3a
Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed 出版商
  • 流式细胞仪; 人类; 图 3a
Li M, Zhang W, Liu J, Li M, Zhang Y, Xiong Y, et al. Dynamic changes in the immunological characteristics of T lymphocytes in surviving patients with severe fever with thrombocytopenia syndrome (SFTS). Int J Infect Dis. 2018;70:72-80 pubmed 出版商
  • 流式细胞仪; 人类; 图 1b
Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed 出版商
  • 流式细胞仪; 人类; 图 2a
Vanegas N, Vernot J. Loss of quiescence and self-renewal capacity of hematopoietic stem cell in an in vitro leukemic niche. Exp Hematol Oncol. 2017;6:2 pubmed 出版商
  • 流式细胞仪; 人类; 图 3b
Ashizawa T, Iizuka A, Nonomura C, Kondou R, Maeda C, Miyata H, et al. Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse. Clin Cancer Res. 2017;23:149-158 pubmed 出版商
  • 流式细胞仪; 人类; 图 s2
Leeansyah E, Svärd J, Dias J, Buggert M, Nyström J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 4
Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed 出版商
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed 出版商
Wang Z, Zhou L, Xiong Y, Yu S, Li H, Fan J, et al. Salinomycin exerts anti-colorectal cancer activity by targeting the β-catenin/T-cell factor complex. Br J Pharmacol. 2019;176:3390-3406 pubmed 出版商
Xiong Y, Zhou L, Su Z, Song J, Sun Q, Liu S, et al. Longdaysin inhibits Wnt/β-catenin signaling and exhibits antitumor activity against breast cancer. Onco Targets Ther. 2019;12:993-1005 pubmed 出版商
Saraiva D, Jacinto A, Borralho P, Braga S, Cabral M. HLA-DR in Cytotoxic T Lymphocytes Predicts Breast Cancer Patients' Response to Neoadjuvant Chemotherapy. Front Immunol. 2018;9:2605 pubmed 出版商
Agrawal N, Streaţă I, Pei G, Weiner J, Kotze L, Bandermann S, et al. Human Monocytic Suppressive Cells Promote Replication of Mycobacterium tuberculosis and Alter Stability of in vitro Generated Granulomas. Front Immunol. 2018;9:2417 pubmed 出版商
Vining K, Scherba J, Bever A, Alexander M, Celiz A, Mooney D. Synthetic Light-Curable Polymeric Materials Provide a Supportive Niche for Dental Pulp Stem Cells. Adv Mater. 2017;: pubmed 出版商
Santos R, Bai L, Singh P, Murakami N, Fan H, Zhan W, et al. Structure of human immunoproteasome with a reversible and noncompetitive inhibitor that selectively inhibits activated lymphocytes. Nat Commun. 2017;8:1692 pubmed 出版商
Bruno T, Ebner P, Moore B, Squalls O, Waugh K, Eruslanov E, et al. Antigen-Presenting Intratumoral B Cells Affect CD4+ TIL Phenotypes in Non-Small Cell Lung Cancer Patients. Cancer Immunol Res. 2017;5:898-907 pubmed 出版商
Zhou H, Mak P, Mu H, Mak D, Zeng Z, Cortes J, et al. Combined inhibition of ?-catenin and Bcr-Abl synergistically targets tyrosine kinase inhibitor-resistant blast crisis chronic myeloid leukemia blasts and progenitors in vitro and in vivo. Leukemia. 2017;31:2065-2074 pubmed 出版商
Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed 出版商
产品信息
抗原 :
Ki67抗原
Apps. Abbrev. :
流式细胞仪细胞内染色, CyTOF®, 免疫细胞化学, 西方墨点法, 免疫组化-F
目录 :
350501
克隆 :
Ki67抗原
产品 :
纯化抗人类Ki67抗原
抗体亚型 :
Mouse IgG1, κ
其他名称 :
抗原Ki67抗原
规格 :
25微克
价格(美元) :
55美元
反应物种 :
Human, Cross-Reactivity: Bovine
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
PURE
免疫原 :
Nuclei of the Hodgkin lymphoma cell line L428
应用注释 :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections 1 , Western blotting 3 , and immunofluorescence microscopy 4 . Ki-67 Staining Protocol: 1. Prepare 70% ethanol and chill at -20°C. 2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350x g for 5 minutes. 3. Discard supernatant and loosen the cell pellet by vortexing. 4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing. 5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour. 6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10 6 /ml. 7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes. 8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。