产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
亮紫色421™抗人类Ki67抗原
目录 :
350505
规格 :
25试验
价格 :
190美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
BV421
克隆名称 :
Ki67抗原
反应物种 :
人类
应用 :
免疫细胞化学, 流式细胞仪
更多信息或购买 :
文章摘录数: 9
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 3:50; 图 3b
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed 出版商
  • 流式细胞仪; 人类; 图 s2
Leeansyah E, Svärd J, Dias J, Buggert M, Nyström J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed 出版商
Taylor H, Calantone N, Lichon D, Hudson H, Clerc I, Campbell E, et al. mTOR Overcomes Multiple Metabolic Restrictions to Enable HIV-1 Reverse Transcription and Intracellular Transport. Cell Rep. 2020;31:107810 pubmed 出版商
Singh M, Jackson K, Wang J, Schofield P, Field M, Koppstein D, et al. Lymphoma Driver Mutations in the Pathogenic Evolution of an Iconic Human Autoantibody. Cell. 2020;180:878-894.e19 pubmed 出版商
Sprouse M, Welte T, Boral D, Liu H, Yin W, Vishnoi M, et al. PMN-MDSCs Enhance CTC Metastatic Properties through Reciprocal Interactions via ROS/Notch/Nodal Signaling. Int J Mol Sci. 2019;20: pubmed 出版商
Vishnoi M, Boral D, Liu H, Sprouse M, Yin W, Goswami Sewell D, et al. Targeting USP7 Identifies a Metastasis-Competent State within Bone Marrow-Resident Melanoma CTCs. Cancer Res. 2018;78:5349-5362 pubmed 出版商
Siriwon N, Kim Y, Siegler E, Chen X, Rohrs J, Liu Y, et al. CAR-T Cells Surface-Engineered with Drug-Encapsulated Nanoparticles Can Ameliorate Intratumoral T-cell Hypofunction. Cancer Immunol Res. 2018;6:812-824 pubmed 出版商
Chierico L, Rizzello L, Guan L, Joseph A, Lewis A, Battaglia G. The role of the two splice variants and extranuclear pathway on Ki-67 regulation in non-cancer and cancer cells. PLoS ONE. 2017;12:e0171815 pubmed 出版商
Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed 出版商
产品信息
抗原 :
Ki67抗原
Apps. Abbrev. :
流式细胞仪细胞内染色, 免疫细胞化学
目录 :
350505
克隆 :
Ki67抗原
产品 :
亮紫色421™抗人类Ki67抗原
抗体亚型 :
Mouse IgG1, κ
其他名称 :
抗原Ki67抗原
规格 :
25试验
价格(美元) :
190美元
反应物种 :
Human, Cross-Reactivity: Bovine
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
BV421
免疫原 :
Nuclei of the Hodgkin lymphoma cell line L428
应用注释 :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections 1 , Western blotting 3 , and immunofluorescence microscopy 4 . Ki-67 Staining Protocol: 1. Prepare 70% ethanol and chill at -20°C. 2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350x g for 5 minutes. 3. Discard supernatant and loosen the cell pellet by vortexing. 4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing. 5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour. 6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10 6 /ml. 7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes. 8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。