产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
Alexa Fluor系列荧光染料®488抗人类Ki67抗原
目录 :
350507
规格 :
25试验
价格 :
115美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
AF488
克隆名称 :
Ki67抗原
反应物种 :
人类, 小鼠
应用 :
免疫细胞化学, 流式细胞仪
更多信息或购买 :
文章摘录数: 14
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 3:50; 图 3b
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed 出版商
  • 流式细胞仪; 人类; 1:20; 图 3a
Huang Y, Huang J, Huang Y, Gan L, Long L, Pu A, et al. TFRC promotes epithelial ovarian cancer cell proliferation and metastasis via up-regulation of AXIN2 expression. Am J Cancer Res. 2020;10:131-147 pubmed
  • 流式细胞仪; 人类; 图 1b
Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed 出版商
  • 流式细胞仪; 人类; 图 s6a
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 3a
Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed 出版商
  • 流式细胞仪; 人类; 图 3a
Li M, Zhang W, Liu J, Li M, Zhang Y, Xiong Y, et al. Dynamic changes in the immunological characteristics of T lymphocytes in surviving patients with severe fever with thrombocytopenia syndrome (SFTS). Int J Infect Dis. 2018;70:72-80 pubmed 出版商
  • 流式细胞仪; 人类; 图 1b
Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed 出版商
Maleki K, Tauriainen J, García M, Kerkman P, Christ W, Dias J, et al. MAIT cell activation is associated with disease severity markers in acute hantavirus infection. Cell Rep Med. 2021;2:100220 pubmed 出版商
Luoma A, Suo S, Williams H, Sharova T, Sullivan K, Manos M, et al. Molecular Pathways of Colon Inflammation Induced by Cancer Immunotherapy. Cell. 2020;: pubmed 出版商
Teijeira A, Garasa S, Gato M, Alfaro C, Miguéliz I, Cirella A, et al. CXCR1 and CXCR2 Chemokine Receptor Agonists Produced by Tumors Induce Neutrophil Extracellular Traps that Interfere with Immune Cytotoxicity. Immunity. 2020;52:856-871.e8 pubmed 出版商
Valpione S, Galvani E, Tweedy J, Mundra P, Banyard A, Middlehurst P, et al. Immune-awakening revealed by peripheral T cell dynamics after one cycle of immunotherapy. Nat Cancer. 2020;1:210-221 pubmed 出版商
Di Blasi D, Boldanova T, Mori L, Terracciano L, Heim M, De Libero G. Unique T-Cell Populations Define Immune-Inflamed Hepatocellular Carcinoma. Cell Mol Gastroenterol Hepatol. 2020;9:195-218 pubmed 出版商
Remes Lenicov F, Paletta A, Gonzalez Prinz M, Varese A, Pavillet C, Lopez Malizia Á, et al. Prostaglandin E2 Antagonizes TGF-β Actions During the Differentiation of Monocytes Into Dendritic Cells. Front Immunol. 2018;9:1441 pubmed 出版商
Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed 出版商
产品信息
抗原 :
Ki67抗原
Apps. Abbrev. :
流式细胞仪细胞内染色, 免疫细胞化学
目录 :
350507
克隆 :
Ki67抗原
产品 :
Alexa Fluor系列荧光染料®488抗人类Ki67抗原
抗体亚型 :
Mouse IgG1, κ
其他名称 :
抗原Ki67抗原
规格 :
25试验
价格(美元) :
115美元
反应物种 :
Human, Cross-Reactivity: Bovine
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
A488
免疫原 :
Nuclei of the Hodgkin lymphoma cell line L428
应用注释 :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections 1 , Western blotting 3 , and immunofluorescence microscopy 4 . Ki-67 Staining Protocol: 1. Prepare 70% ethanol and chill at -20°C. 2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350x g for 5 minutes. 3. Discard supernatant and loosen the cell pellet by vortexing. 4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing. 5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour. 6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10 6 /ml. 7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes. 8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。