产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
APC抗人类Ki67抗原
目录 :
350514
规格 :
100试验
价格 :
326美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
APC
克隆名称 :
Ki67抗原
反应物种 :
人类
应用 :
流式细胞仪
更多信息或购买 :
BioLegend 产品网页
文章摘录数: 13
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 图 2a
Vanegas N, Vernot J. Loss of quiescence and self-renewal capacity of hematopoietic stem cell in an in vitro leukemic niche. Exp Hematol Oncol. 2017;6:2 pubmed 出版商
Dubuisson A, Fahrner J, Goubet A, Terrisse S, Voisin N, Bayard C, et al. Immunodynamics of explanted human tumors for immuno-oncology. EMBO Mol Med. 2021;13:e12850 pubmed 出版商
Jung Y, Cackowski F, Yumoto K, Decker A, Wang Y, Hotchkin M, et al. Abscisic acid regulates dormancy of prostate cancer disseminated tumor cells in the bone marrow. Neoplasia. 2021;23:102-111 pubmed 出版商
Zhou W, Wang H, Zhang J, Dai H, Yao Z, Zheng Z, et al. NEAT1/miR-200b-3p/SMAD2 axis promotes progression of melanoma. Aging (Albany NY). 2020;12:22759-22775 pubmed 出版商
Evans R, Antonelou M, Sathiananthamoorthy S, Rega M, Henderson S, Ceron Gutierrez L, et al. Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nat Commun. 2020;11:4368 pubmed 出版商
Landgraf K, Williams S, Steiger D, Gebhart D, Lok S, Martin D, et al. convertibleCARs: A chimeric antigen receptor system for flexible control of activity and antigen targeting. Commun Biol. 2020;3:296 pubmed 出版商
Wang D, Liu N, Xie Y, Song B, Kong S, Sun X. Different culture method changing CD105 expression in amniotic fluid MSCs without affecting differentiation ability or immune function. J Cell Mol Med. 2020;24:4212-4222 pubmed 出版商
Sontheimer Phelps A, Chou D, Tovaglieri A, Ferrante T, Duckworth T, Fadel C, et al. Human Colon-on-a-Chip Enables Continuous In Vitro Analysis of Colon Mucus Layer Accumulation and Physiology. Cell Mol Gastroenterol Hepatol. 2020;9:507-526 pubmed 出版商
Wu K, Mu X, Jiang J, Tan M, Wang R, Zhou W, et al. miRNA‑26a‑5p and miR‑26b‑5p inhibit the proliferation of bladder cancer cells by regulating PDCD10. Oncol Rep. 2018;40:3523-3532 pubmed 出版商
Yumoto K, Eber M, Wang J, Cackowski F, Decker A, Lee E, et al. Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow. Sci Rep. 2016;6:36520 pubmed 出版商
Nodomi S, Umeda K, Saida S, Kinehara T, Hamabata T, Daifu T, et al. CD146 is a novel marker for highly tumorigenic cells and a potential therapeutic target in malignant rhabdoid tumor. Oncogene. 2016;35:5317-5327 pubmed 出版商
Maine C, Marquardt K, Cheung J, Sherman L. PTPN22 controls the germinal center by influencing the numbers and activity of T follicular helper cells. J Immunol. 2014;192:1415-24 pubmed 出版商
Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed 出版商
产品信息
抗原 :
Ki67抗原
Apps. Abbrev. :
流式细胞仪细胞内染色
目录 :
350514
克隆 :
Ki67抗原
产品 :
APC抗人类Ki67抗原
抗体亚型 :
Mouse IgG1, κ
其他名称 :
抗原Ki67抗原
规格 :
100试验
价格(美元) :
326美元
反应物种 :
人类
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
APC
免疫原 :
Nuclei of the Hodgkin lymphoma cell line L428
应用注释 :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections 1 , Western blotting 3 , and immunofluorescence microscopy 4 . Ki-67 Staining Protocol: 1. Prepare 70% ethanol and chill at -20 C. 2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350x g for 5 minutes. 3. Discard supernatant and loosen the cell pellet by vortexing. 4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing. 5. Continue vortexing for 30 seconds and then incubate at -20 C for 1 hour. 6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10 6 /ml. 7. Mix 100 ul cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes. 8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
更多信息或购买 :
BioLegend 产品网页
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品，旨在加速生命科学领域的研发工作。