产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
亮紫色711™抗人类Ki67抗原
目录 :
350516
规格 :
100试验
价格 :
405美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
BV711
克隆名称 :
Ki67抗原
反应物种 :
人类, 小鼠
应用 :
流式细胞仪
更多信息或购买 :
文章摘录数: 8
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 图 4s1
Pallikkuth S, Chaudhury S, Lu P, Pan L, Jongert E, Wille Reece U, et al. A delayed fractionated dose RTS,S AS01 vaccine regimen mediates protection via improved T follicular helper and B cell responses. elife. 2020;9: pubmed 出版商
  • 流式细胞仪; 人类; 图 1b
Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 4
Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed 出版商
Giron L, Colomb F, Papasavvas E, Azzoni L, Yin X, Fair M, et al. Interferon-α alters host glycosylation machinery during treated HIV infection. EBioMedicine. 2020;59:102945 pubmed 出版商
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed 出版商
Jimeno R, Leceta J, Garín M, Ortiz A, Mellado M, Rodríguez Frade J, et al. Th17 polarization of memory Th cells in early arthritis: the vasoactive intestinal peptide effect. J Leukoc Biol. 2015;98:257-69 pubmed 出版商
van Aalderen M, Remmerswaal E, Verstegen N, Hombrink P, ten Brinke A, Pircher H, et al. Infection history determines the differentiation state of human CD8+ T cells. J Virol. 2015;89:5110-23 pubmed 出版商
Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed 出版商
产品信息
抗原 :
Ki67抗原
Apps. Abbrev. :
流式细胞仪细胞内染色
目录 :
350516
克隆 :
Ki67抗原
产品 :
亮紫色711™抗人类Ki67抗原
抗体亚型 :
Mouse IgG1, κ
其他名称 :
抗原Ki67抗原
规格 :
100试验
价格(美元) :
405美元
反应物种 :
Human, Cross-Reactivity: Bovine
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
BV711
免疫原 :
Nuclei of the Hodgkin lymphoma cell line L428
应用注释 :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections 1 , Western blotting 3 , and immunofluorescence microscopy 4 . Ki-67 Staining Protocol: 1. Prepare 70% ethanol and chill at -20 C. 2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350x g for 5 minutes. 3. Discard supernatant and loosen the cell pellet by vortexing. 4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing. 5. Continue vortexing for 30 seconds and then incubate at -20 C for 1 hour. 6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10 6 /ml. 7. Mix 100 ul cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes. 8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。