Babco, Signet, Sternberger Monoclonals, Senetek, Covance

抗体

亮紫色510™抗人类Ki67抗原

350517

25试验

215美元

单克隆

小鼠

BV510

Ki67抗原

流式细胞仪细胞内染色, 免疫细胞化学

350517

Ki67抗原

亮紫色510™抗人类Ki67抗原

Mouse IgG1, κ

抗原Ki67抗原

25试验

215美元

人类

单克隆

小鼠

BV510

Nuclei of the Hodgkin lymphoma cell line L428

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections 1 , Western blotting 3 , and immunofluorescence microscopy 4 . Ki-67 Staining Protocol: 1. Prepare 70% ethanol and chill at -20 C. 2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350x g for 5 minutes. 3. Discard supernatant and loosen the cell pellet by vortexing. 4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing. 5. Continue vortexing for 30 seconds and then incubate at -20 C for 1 hour. 6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10 6 /ml. 7. Mix 100 ul cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes. 8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.