产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
Purified anti-human Ki-67 (Maxpar® Ready)
目录 :
350523
规格 :
100微克
价格 :
171美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
Ki67抗原
反应物种 :
人类, 小鼠
应用 :
流式细胞仪
更多信息或购买 :
文章摘录数: 19
出版应用/物种/样本/稀释 | 参考文献 |
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| Huang Y, Huang J, Huang Y, Gan L, Long L, Pu A, et al. TFRC promotes epithelial ovarian cancer cell proliferation and metastasis via up-regulation of AXIN2 expression. Am J Cancer Res. 2020;10:131-147 pubmed
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| Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed 出版商
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产品信息
抗原 :
Ki67抗原
Apps. Abbrev. :
流式细胞仪细胞内染色, CyTOF®
目录 :
350523
克隆 :
Ki67抗原
产品 :
Purified anti-human Ki-67 (Maxpar® Ready)
抗体亚型 :
Mouse IgG1, κ
其他名称 :
抗原Ki67抗原
规格 :
100微克
价格(美元) :
171美元
反应物种 :
人类
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
PUREMAX
免疫原 :
Nuclei of the Hodgkin lymphoma cell line L428
应用注释 :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections 1 , Western blotting 3 , and immunofluorescence microscopy 4 . Ki-67 Staining Protocol: 1. Prepare 70% ethanol and chill at -20 C. 2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350x g for 5 minutes. 3. Discard supernatant and loosen the cell pellet by vortexing. 4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing. 5. Continue vortexing for 30 seconds and then incubate at -20 C for 1 hour. 6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10 6 /ml. 7. Mix 100 ul cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes. 8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。