产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
PE/Cyanine7抗人类Ki67抗原
目录 :
350525
规格 :
25试验
价格 :
129美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
PE/Cy7
克隆名称 :
Ki67抗原
反应物种 :
人类
应用 :
流式细胞仪
更多信息或购买 :
文章摘录数: 7
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 图 3b
Ashizawa T, Iizuka A, Nonomura C, Kondou R, Maeda C, Miyata H, et al. Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse. Clin Cancer Res. 2017;23:149-158 pubmed 出版商
Wong S, Oshansky C, Guo X, Ralston J, Wood T, Reynolds G, et al. Activated CD4+ T cells and CD14hiCD16+ monocytes correlate with antibody response following influenza virus infection in humans. Cell Rep Med. 2021;2:100237 pubmed 出版商
Rodriguez A, Zhang K, Färkkilä A, Filiatrault J, Yang C, Velázquez M, et al. MYC Promotes Bone Marrow Stem Cell Dysfunction in Fanconi Anemia. Cell Stem Cell. 2021;28:33-47.e8 pubmed 出版商
Wang J, Xu Y, Chen Z, Liang J, Lin Z, Liang H, et al. Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia. Cell. 2020;183:1867-1883.e26 pubmed 出版商
Satoh Takayama N, Kato T, Motomura Y, Kageyama T, Taguchi Atarashi N, Kinoshita Daitoku R, et al. Bacteria-Induced Group 2 Innate Lymphoid Cells in the Stomach Provide Immune Protection through Induction of IgA. Immunity. 2020;52:635-649.e4 pubmed 出版商
Zhong W, Xu M, Li C, Zhu B, Cao X, Li D, et al. ORP4L Extracts and Presents PIP2 from Plasma Membrane for PLCβ3 Catalysis: Targeting It Eradicates Leukemia Stem Cells. Cell Rep. 2019;26:2166-2177.e9 pubmed 出版商
Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed 出版商
产品信息
抗原 :
Ki67抗原
Apps. Abbrev. :
流式细胞仪细胞内染色
目录 :
350525
克隆 :
Ki67抗原
产品 :
PE/Cyanine7抗人类Ki67抗原
抗体亚型 :
Mouse IgG1, κ
其他名称 :
抗原Ki67抗原
规格 :
25试验
价格(美元) :
129美元
反应物种 :
人类
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
PE_CYANINE7
免疫原 :
Nuclei of the Hodgkin lymphoma cell line L428
应用注释 :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections 1 , Western blotting 3 , and immunofluorescence microscopy 4 . Ki-67 Staining Protocol: 1. Prepare 70% ethanol and chill at -20 C. 2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350x g for 5 minutes. 3. Discard supernatant and loosen the cell pellet by vortexing. 4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing. 5. Continue vortexing for 30 seconds and then incubate at -20 C for 1 hour. 6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10 6 /ml. 7. Mix 100 ul cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes. 8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。