产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
其他
产品名称 :
4',6-二脒基-2-苯基吲哚
目录 :
422801
规格 :
10毫克
价格 :
85美元
更多信息或购买 :
文章摘录数: 51
参考文献
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Pagan J, Kitaoka M, Anthony R. Engineered Sialylation of Pathogenic Antibodies In Vivo Attenuates Autoimmune Disease. Cell. 2018;172:564-577.e13 pubmed 出版商
Yanting C, Yang Q, Ma G, Du M, Harrison J, Block E. Dose- and type-dependent effects of long-chain fatty acids on adipogenesis and lipogenesis of bovine adipocytes. J Dairy Sci. 2018;101:1601-1615 pubmed 出版商
Schmidt A, Steinritz D, Rothmiller S, Thiermann H, Scherer A. Effects of sulfur mustard on mesenchymal stem cells. Toxicol Lett. 2018;293:98-104 pubmed 出版商
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Chmielewski M, Abken H. CAR T Cells Releasing IL-18 Convert to T-Bethigh FoxO1low Effectors that Exhibit Augmented Activity against Advanced Solid Tumors. Cell Rep. 2017;21:3205-3219 pubmed 出版商
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Doorley L, LeMessurier K, Iverson A, Palipane M, Samarasinghe A. Humoral immune responses during asthma and influenza co-morbidity in mice. Immunobiology. 2017;222:1064-1073 pubmed 出版商
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产品信息
抗原 :
4',6-二脒基-2-苯基吲哚
Apps. Abbrev. :
流式细胞仪细胞内染色, 免疫细胞化学
目录 :
422801
产品 :
4',6-二脒基-2-苯基吲哚
其他名称 :
4',6-Diamidino-2-Phenylindole, Dilactate
规格 :
10毫克
价格(美元) :
85美元
共轭标签/标签/标记 :
NONAB_CHMPRB
应用注释 :
Protocols for Counterstaining with DAPI: Immunofluorescence Staining Note: Cells may be fixed by method of choice, such as with 4% paraformaldehyde. DAPI staining is done after staining for other markers. Fixation and permeabilization of cells is not necessary to counterstain with DAPI. 1. Fix cells using method of choice. 2. Incubate the cells with phosphate buffered saline (PBS) for 15 minutes. 3. Dilute the DAPI solution (we recommend to 300 nM) with PBS. Remove excess PBS from the slide and cover with DAPI solution, making sure the cells are completely covered. 4. Incubate for up to 5 minutes. Rinse the slide several times to remove all free DAPI. It is recommended to use a mounting medium with antifade reagent to reduce fluorescence quenching. The slide is ready to view under the microscope with appropriate filters. Nuclear Staining for Flow Cytometry Note: Cells may be fixed with 4% paraformadehyde or absolute ethanol. 1. Fix cells with absolute ethanol or 4% paraformaldehyde. 2. Centrifuge, discard the supernatant, and add 5 ml of PBS, allowing cells to rehydrate for 15 minutes. 3. Dilute the DAPI to 3 uM (recommended) in staining buffer (such as BioLegend s FOXP3 Perm Buffer, Cat. No. 421402). 4. Centrifuge cell suspension from step 3 above, discard supernatant, and add 1 ml of DAPI solution; then incubate at room temperature for 15 minutes. 5. Analyze by flow cytometry using an instrument with appropriate lasers.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。