Babco, Signet, Sternberger Monoclonals, Senetek, Covance

ELISA/assay

Veri-Cells™ PBMC

425004

50试验

275美元

425004

Veri-Cells™ PBMC

Control cells

50试验

275美元

套

The Veri-Cells PBMC Kit can be used as positive controls for surface immunofluorescent staining and multi-color flow cytometry assays using the BioLegend surface staining protocol. It can also be used for the detection of intracellular molecules such as Granzyme B, Perforin, Foxp3 and Helios using BioLegend s Fixation Buffer and Intracellular Staining Permeabilization Wash Buffer (10X) or the True Nuclear Transcription Factor Buffer Set. For best results, Cell Staining Buffer is recommended for any washing steps. Product performance specifications are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance. Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells PBMC. The antibodies used for testing were the following: CD56 PE (clone 5.1H11), CD16 PE (clone 3G8), CD19 APC (clone HIB19), CD3 FITC (clone UCHT1), CD4 PE/Cy7 (clone RPA-T4), CD8 APC/Cy7 (clone RPA-T8). Percent positive is based on gates set on unstained cells. The Certificate of Analysis specific values are provided only as a reference. Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.