Babco, Signet, Sternberger Monoclonals, Senetek, Covance

抗体

生物素抗人类TNF-α

502904

500微克

273美元

单克隆

小鼠

生物素

MAb11

酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

ELISA Detection, ICC, ELISPOT Detection, Intracellular Staining for Flow Cytometry, Flow Cytometry

502904

MAb11

生物素抗人类TNF-α

Mouse IgG1, κ

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2

500微克

273美元

人类

单克隆

小鼠

生物素

E. coli -expressed, recombinant human TNF-α

ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody. Flow Cytometry 3,5,6,10 : The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations. Additional reported applications (for the relevant formats) include: neutralization 1,2 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 4 and acetone-fixed frozen tissue sections 8 , immunocytochemistry 7 , and immunofluorescence 9 . The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a. Note: For testing human TNF-a in serum or plasma, BioLegend s ELISA Max Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922). The Purified MAb1 antibody is useful in neutralization 2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody. Clone MAb11 cross-reacts to Cat 11