产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
LEAF™纯化抗人类TNF-α
目录 :
502922
规格 :
500微克
价格 :
250美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
MAb11
反应物种 :
人类, 猕猴
应用 :
酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 中和反应, 流式细胞仪
更多信息或购买 :
文章摘录数: 39
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 图 1b
Meckiff B, Ladell K, McLaren J, Ryan G, Leese A, James E, et al. Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells. J Immunol. 2019;203:1276-1287 pubmed 出版商
  • 流式细胞仪; 人类; 图 3b
van de Garde M, van Westen E, Poelen M, Rots N, van Els C. Prediction and Validation of Immunogenic Domains of Pneumococcal Proteins Recognized by Human CD4+ T Cells. Infect Immun. 2019;87: pubmed 出版商
  • 流式细胞仪; 人类; 图 8d
Aulicino A, Rue Albrecht K, Preciado Llanes L, Napolitani G, Ashley N, Cribbs A, et al. Invasive Salmonella exploits divergent immune evasion strategies in infected and bystander dendritic cell subsets. Nat Commun. 2018;9:4883 pubmed 出版商
  • 流式细胞仪; 人类; 图 3b
Li N, van Unen V, Höllt T, Thompson A, van Bergen J, Pezzotti N, et al. Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine. J Exp Med. 2018;215:1383-1396 pubmed 出版商
  • 流式细胞仪; 人类; 图 2h
Pizzolla A, Nguyen T, Sant S, Jaffar J, Loudovaris T, Mannering S, et al. Influenza-specific lung-resident memory T cells are proliferative and polyfunctional and maintain diverse TCR profiles. J Clin Invest. 2018;128:721-733 pubmed 出版商
  • 流式细胞仪; 人类; 图 s1e
Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed 出版商
  • 流式细胞仪; 人类; 图 6a
Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed 出版商
  • 流式细胞仪; 人类; 图 3a
Duggal N, Beswetherick A, Upton J, Hampson P, Phillips A, Lord J. Depressive symptoms in hip fracture patients are associated with reduced monocyte superoxide production. Exp Gerontol. 2014;54:27-34 pubmed 出版商
Cai D, Li J, Liu D, Hong S, Qiao Q, Sun Q, et al. Tumor-expressed B7-H3 mediates the inhibition of antitumor T-cell functions in ovarian cancer insensitive to PD-1 blockade therapy. Cell Mol Immunol. 2019;: pubmed 出版商
Pallikkuth S, de Armas L, Rinaldi S, George V, Pan L, Arheart K, et al. Dysfunctional peripheral T follicular helper cells dominate in people with impaired influenza vaccine responses: Results from the FLORAH study. PLoS Biol. 2019;17:e3000257 pubmed 出版商
Schlenner S, Pasciuto E, Lagou V, Burton O, Prezzemolo T, Junius S, et al. NFIL3 mutations alter immune homeostasis and sensitise for arthritis pathology. Ann Rheum Dis. 2019;78:342-349 pubmed 出版商
Kim C, Hu B, Jadhav R, Jin J, Zhang H, Cavanagh M, et al. Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells. Cell Rep. 2018;25:2148-2162.e5 pubmed 出版商
Chen H, van der Touw W, Wang Y, Kang K, Mai S, Zhang J, et al. Blocking immunoinhibitory receptor LILRB2 reprograms tumor-associated myeloid cells and promotes antitumor immunity. J Clin Invest. 2018;128:5647-5662 pubmed 出版商
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed 出版商
Kauffman K, Sallin M, Hoft S, Sakai S, Moore R, Wilder Kofie T, et al. Limited Pulmonary Mucosal-Associated Invariant T Cell Accumulation and Activation during Mycobacterium tuberculosis Infection in Rhesus Macaques. Infect Immun. 2018;86: pubmed 出版商
Petrelli A, Mijnheer G, Hoytema van Konijnenburg D, van der Wal M, Giovannone B, Mocholí E, et al. PD-1+CD8+ T cells are clonally expanding effectors in human chronic inflammation. J Clin Invest. 2018;128:4669-4681 pubmed 出版商
Wang H, D Souza C, Lim X, Kostenko L, Pediongco T, Eckle S, et al. MAIT cells protect against pulmonary Legionella longbeachae infection. Nat Commun. 2018;9:3350 pubmed 出版商
Cichocki F, Wu C, Zhang B, Felices M, Tesi B, Tuininga K, et al. ARID5B regulates metabolic programming in human adaptive NK cells. J Exp Med. 2018;215:2379-2395 pubmed 出版商
Levin M, Kroehl M, Johnson M, Hammes A, Reinhold D, Lang N, et al. Th1 memory differentiates recombinant from live herpes zoster vaccines. J Clin Invest. 2018;128:4429-4440 pubmed 出版商
Kirkling M, Cytlak U, Lau C, Lewis K, Resteu A, Khodadadi Jamayran A, et al. Notch Signaling Facilitates In Vitro Generation of Cross-Presenting Classical Dendritic Cells. Cell Rep. 2018;23:3658-3672.e6 pubmed 出版商
Ramsingh A, Gray S, Reilly A, Koday M, Bratt D, Koday M, et al. Sustained AAV9-mediated expression of a non-self protein in the CNS of non-human primates after immunomodulation. PLoS ONE. 2018;13:e0198154 pubmed 出版商
Holzer B, Morgan S, Matsuoka Y, Edmans M, Salguero F, Everett H, et al. Comparison of Heterosubtypic Protection in Ferrets and Pigs Induced by a Single-Cycle Influenza Vaccine. J Immunol. 2018;200:4068-4077 pubmed 出版商
Miles J, Tan M, Dolton G, Edwards E, Galloway S, Laugel B, et al. Peptide mimic for influenza vaccination using nonnatural combinatorial chemistry. J Clin Invest. 2018;128:1569-1580 pubmed 出版商
Chen D, Xie J, Fiskesund R, Dong W, Liang X, Lv J, et al. Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype. Nat Commun. 2018;9:873 pubmed 出版商
Chang Z, Lorenzini M, Chen X, Tran U, Bangayan N, Chen Y. Rewiring T-cell responses to soluble factors with chimeric antigen receptors. Nat Chem Biol. 2018;14:317-324 pubmed 出版商
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产品信息
抗原 :
肿瘤坏死因子甲
Apps. Abbrev. :
ELISA, CyTOF®, Flow Cytometry, Intracellular Staining for Flow Cytometry, ICC, Immunofluorescence Microscopy, IHC, Neut
目录 :
502922
克隆 :
MAb11
产品 :
LEAF™纯化抗人类TNF-α
抗体亚型 :
Mouse IgG1, κ
其他名称 :
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
规格 :
500微克
价格(美元) :
250美元
反应物种 :
Human, Cat (Feline), Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Swine (Pig, Porcine), Pigtailed Macaque, Sooty Mangabey
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
LEAF
免疫原 :
E. coli -expressed, recombinant human TNF-α
应用注释 :
ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody. Flow Cytometry 3,5,6,10 : The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-α-producing cells within mixed cell populations. Additional reported applications (for the relevant formats) include: neutralization 1,2 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 4 and acetone-fixed frozen tissue sections 8 , immunocytochemistry 7 , and immunofluorescence 9 . The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-α. Note: For testing human TNF-α in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human TNF-α bioactivity (Cat. No. 502922). The Purified MAb1 antibody is useful in neutralization 2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。