产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
Alexa Fluor系列荧光染料®700抗人类TNF-α
目录 :
502928
规格 :
100试验
价格 :
288美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
AF700
克隆名称 :
MAb11
反应物种 :
人类
应用 :
流式细胞仪, mass cytometry
更多信息或购买 :
文章摘录数: 57
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 图 4i
Hickman T, Choi E, Whiteman K, Muralidharan S, Pai T, Johnson T, et al. BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity. PLoS ONE. 2022;17:e0266980 pubmed 出版商
  • mass cytometry; 人类; 图 4e
  • 流式细胞仪; 人类; 图 2d
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed 出版商
  • 流式细胞仪; 人类; 3:50; 图 4a
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed 出版商
  • 流式细胞仪; 人类; 图 4b
Marotte L, Simon S, Vignard V, Dupré E, Gantier M, Cruard J, et al. Increased antitumor efficacy of PD-1-deficient melanoma-specific human lymphocytes. J Immunother Cancer. 2020;8: pubmed 出版商
  • 流式细胞仪; 人类; 图 6d
Lynn R, Weber E, Sotillo E, Gennert D, Xu P, Good Z, et al. c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature. 2019;576:293-300 pubmed 出版商
  • 流式细胞仪; 人类; 图 1b
Meckiff B, Ladell K, McLaren J, Ryan G, Leese A, James E, et al. Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells. J Immunol. 2019;203:1276-1287 pubmed 出版商
  • 流式细胞仪; 人类; 图 3b
van de Garde M, van Westen E, Poelen M, Rots N, van Els C. Prediction and Validation of Immunogenic Domains of Pneumococcal Proteins Recognized by Human CD4+ T Cells. Infect Immun. 2019;87: pubmed 出版商
  • 流式细胞仪; 人类; 图 6a
Banki Z, Krabbendam L, Klaver D, Leng T, Kruis S, Mehta H, et al. Antibody opsonization enhances MAIT cell responsiveness to bacteria via a TNF-dependent mechanism. Immunol Cell Biol. 2019;97:538-551 pubmed 出版商
  • 流式细胞仪; 人类; 图 3f
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed 出版商
  • 流式细胞仪; 人类; 图 8d
Aulicino A, Rue Albrecht K, Preciado Llanes L, Napolitani G, Ashley N, Cribbs A, et al. Invasive Salmonella exploits divergent immune evasion strategies in infected and bystander dendritic cell subsets. Nat Commun. 2018;9:4883 pubmed 出版商
  • 流式细胞仪; 人类; 1:50; 图 1e
Wagner D, Amini L, Wendering D, Burkhardt L, Akyüz L, Reinke P, et al. High prevalence of Streptococcus pyogenes Cas9-reactive T cells within the adult human population. Nat Med. 2019;25:242-248 pubmed 出版商
  • 流式细胞仪; 人类; 图 3b
Li N, van Unen V, Höllt T, Thompson A, van Bergen J, Pezzotti N, et al. Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine. J Exp Med. 2018;215:1383-1396 pubmed 出版商
  • 流式细胞仪; 人类; 图 2h
Pizzolla A, Nguyen T, Sant S, Jaffar J, Loudovaris T, Mannering S, et al. Influenza-specific lung-resident memory T cells are proliferative and polyfunctional and maintain diverse TCR profiles. J Clin Invest. 2018;128:721-733 pubmed 出版商
  • 流式细胞仪; 人类; 图 s1e
Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed 出版商
  • 流式细胞仪; 人类; 图 6a
Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed 出版商
  • 流式细胞仪; 人类; 图 1
Boyle M, Jagannathan P, Bowen K, McIntyre T, Vance H, Farrington L, et al. Effector Phenotype of Plasmodium falciparum-Specific CD4+ T Cells Is Influenced by Both Age and Transmission Intensity in Naturally Exposed Populations. J Infect Dis. 2015;212:416-25 pubmed 出版商
Zhang P, Narayanan E, Liu Q, Tsybovsky Y, Boswell K, Ding S, et al. A multiclade env-gag VLP mRNA vaccine elicits tier-2 HIV-1-neutralizing antibodies and reduces the risk of heterologous SHIV infection in macaques. Nat Med. 2021;27:2234-2245 pubmed 出版商
Pan Y, Aiamkitsumrit B, Bartolo L, Wang Y, Lavery C, Marc A, et al. Vaccination reshapes the virus-specific T cell repertoire in unexposed adults. Immunity. 2021;54:1245-1256.e5 pubmed 出版商
Stephenson E, Reynolds G, Botting R, Calero Nieto F, Morgan M, Tuong Z, et al. Single-cell multi-omics analysis of the immune response in COVID-19. Nat Med. 2021;27:904-916 pubmed 出版商
Gruber C, Calis J, Buta S, Evrony G, Martin J, Uhl S, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity. 2020;53:672-684.e11 pubmed 出版商
Luo X, Cui H, Cai L, Zhu W, Yang W, PATRICK M, et al. Selection of a Clinical Lead TCR Targeting Alpha-Fetoprotein-Positive Liver Cancer Based on a Balance of Risk and Benefit. Front Immunol. 2020;11:623 pubmed 出版商
Ali A, Chiuppesi F, Nguyen M, Hausner M, Nguyen J, Kha M, et al. Chimeric Antigen Receptors Targeting Human Cytomegalovirus. J Infect Dis. 2020;222:853-862 pubmed 出版商
Rodriguez Garcia A, Sharma P, Poussin M, Boesteanu A, Minutolo N, Gitto S, et al. CAR T Cells Targeting MISIIR for the Treatment of Ovarian Cancer and Other Gynecologic Malignancies. Mol Ther. 2020;28:548-560 pubmed 出版商
Cogswell A, Maski K, Scammell T, Tucker D, Orban Z, Koralnik I. Children with Narcolepsy type 1 have increased T-cell responses to orexins. Ann Clin Transl Neurol. 2019;6:2566-2572 pubmed 出版商
Schlenner S, Pasciuto E, Lagou V, Burton O, Prezzemolo T, Junius S, et al. NFIL3 mutations alter immune homeostasis and sensitise for arthritis pathology. Ann Rheum Dis. 2019;78:342-349 pubmed 出版商
Kim C, Hu B, Jadhav R, Jin J, Zhang H, Cavanagh M, et al. Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells. Cell Rep. 2018;25:2148-2162.e5 pubmed 出版商
Chen H, van der Touw W, Wang Y, Kang K, Mai S, Zhang J, et al. Blocking immunoinhibitory receptor LILRB2 reprograms tumor-associated myeloid cells and promotes antitumor immunity. J Clin Invest. 2018;128:5647-5662 pubmed 出版商
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed 出版商
Kauffman K, Sallin M, Hoft S, Sakai S, Moore R, Wilder Kofie T, et al. Limited Pulmonary Mucosal-Associated Invariant T Cell Accumulation and Activation during Mycobacterium tuberculosis Infection in Rhesus Macaques. Infect Immun. 2018;86: pubmed 出版商
Petrelli A, Mijnheer G, Hoytema van Konijnenburg D, van der Wal M, Giovannone B, Mocholí E, et al. PD-1+CD8+ T cells are clonally expanding effectors in human chronic inflammation. J Clin Invest. 2018;128:4669-4681 pubmed 出版商
Wang H, D Souza C, Lim X, Kostenko L, Pediongco T, Eckle S, et al. MAIT cells protect against pulmonary Legionella longbeachae infection. Nat Commun. 2018;9:3350 pubmed 出版商
Cichocki F, Wu C, Zhang B, Felices M, Tesi B, Tuininga K, et al. ARID5B regulates metabolic programming in human adaptive NK cells. J Exp Med. 2018;215:2379-2395 pubmed 出版商
Levin M, Kroehl M, Johnson M, Hammes A, Reinhold D, Lang N, et al. Th1 memory differentiates recombinant from live herpes zoster vaccines. J Clin Invest. 2018;128:4429-4440 pubmed 出版商
Kirkling M, Cytlak U, Lau C, Lewis K, Resteu A, Khodadadi Jamayran A, et al. Notch Signaling Facilitates In Vitro Generation of Cross-Presenting Classical Dendritic Cells. Cell Rep. 2018;23:3658-3672.e6 pubmed 出版商
Ramsingh A, Gray S, Reilly A, Koday M, Bratt D, Koday M, et al. Sustained AAV9-mediated expression of a non-self protein in the CNS of non-human primates after immunomodulation. PLoS ONE. 2018;13:e0198154 pubmed 出版商
Kim M, Yu K, Kenderian S, Ruella M, Chen S, Shin T, et al. Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia. Cell. 2018;173:1439-1453.e19 pubmed 出版商
Holzer B, Morgan S, Matsuoka Y, Edmans M, Salguero F, Everett H, et al. Comparison of Heterosubtypic Protection in Ferrets and Pigs Induced by a Single-Cycle Influenza Vaccine. J Immunol. 2018;200:4068-4077 pubmed 出版商
Wing A, Fajardo C, Posey A, Shaw C, Da T, Young R, et al. Improving CART-Cell Therapy of Solid Tumors with Oncolytic Virus-Driven Production of a Bispecific T-cell Engager. Cancer Immunol Res. 2018;6:605-616 pubmed 出版商
Miles J, Tan M, Dolton G, Edwards E, Galloway S, Laugel B, et al. Peptide mimic for influenza vaccination using nonnatural combinatorial chemistry. J Clin Invest. 2018;128:1569-1580 pubmed 出版商
Chen D, Xie J, Fiskesund R, Dong W, Liang X, Lv J, et al. Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype. Nat Commun. 2018;9:873 pubmed 出版商
Chang Z, Lorenzini M, Chen X, Tran U, Bangayan N, Chen Y. Rewiring T-cell responses to soluble factors with chimeric antigen receptors. Nat Chem Biol. 2018;14:317-324 pubmed 出版商
Wu H, Wiseman R, Hughes C, Webb G, Abdulhaqq S, Bimber B, et al. The Role of MHC-E in T Cell Immunity Is Conserved among Humans, Rhesus Macaques, and Cynomolgus Macaques. J Immunol. 2018;200:49-60 pubmed 出版商
Prezzemolo T, van Meijgaarden K, Franken K, Caccamo N, Dieli F, Ottenhoff T, et al. Detailed characterization of human Mycobacterium tuberculosis specific HLA-E restricted CD8+ T-cells. Eur J Immunol. 2017;: pubmed 出版商
Hydes T, Noll A, Salinas Riester G, Abuhilal M, Armstrong T, Hamady Z, et al. IL-12 and IL-15 induce the expression of CXCR6 and CD49a on peripheral natural killer cells. Immun Inflamm Dis. 2018;6:34-46 pubmed 出版商
Teigler J, Zelinskyy G, Eller M, Bolton D, Marovich M, Gordon A, et al. Differential Inhibitory Receptor Expression on T Cells Delineates Functional Capacities in Chronic Viral Infection. J Virol. 2017;91: pubmed 出版商
Cichocki F, Valamehr B, Bjordahl R, Zhang B, Rezner B, Rogers P, et al. GSK3 Inhibition Drives Maturation of NK Cells and Enhances Their Antitumor Activity. Cancer Res. 2017;77:5664-5675 pubmed 出版商
Schwerd T, Twigg S, Aschenbrenner D, Manrique S, Miller K, Taylor I, et al. A biallelic mutation in IL6ST encoding the GP130 co-receptor causes immunodeficiency and craniosynostosis. J Exp Med. 2017;214:2547-2562 pubmed 出版商
Almeida V, Avelar Freitas B, Santos M, Costa L, Silva T, Pereira W, et al. Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-? and TNF-? production in vitro. Braz J Med Biol Res. 2017;50:e5163 pubmed 出版商
Ott P, Hu Z, Keskin D, Shukla S, Sun J, Bozym D, et al. An immunogenic personal neoantigen vaccine for patients with melanoma. Nature. 2017;547:217-221 pubmed 出版商
Cao D, Cao Q, Subramaniam S, Yugo D, Heffron C, Rogers A, et al. Pig model mimicking chronic hepatitis E virus infection in immunocompromised patients to assess immune correlates during chronicity. Proc Natl Acad Sci U S A. 2017;114:6914-6923 pubmed 出版商
Qaqish A, Huang D, Chen C, Zhang Z, Wang R, Li S, et al. Adoptive Transfer of Phosphoantigen-Specific ?? T Cell Subset Attenuates Mycobacterium tuberculosis Infection in Nonhuman Primates. J Immunol. 2017;198:4753-4763 pubmed 出版商
Longbrake E, Cantoni C, Chahin S, Cignarella F, Cross A, Piccio L. Dimethyl fumarate induces changes in B- and T-lymphocyte function independent of the effects on absolute lymphocyte count. Mult Scler. 2018;24:728-738 pubmed 出版商
Huang A, Postow M, Orlowski R, Mick R, Bengsch B, Manne S, et al. T-cell invigoration to tumour burden ratio associated with anti-PD-1 response. Nature. 2017;545:60-65 pubmed 出版商
Izawa K, Martin E, Soudais C, Bruneau J, Boutboul D, Rodriguez R, et al. Inherited CD70 deficiency in humans reveals a critical role for the CD70-CD27 pathway in immunity to Epstein-Barr virus infection. J Exp Med. 2017;214:73-89 pubmed 出版商
Sekine T, Marin D, Cao K, Li L, Mehta P, Shaim H, et al. Specific combinations of donor and recipient KIR-HLA genotypes predict for large differences in outcome after cord blood transplantation. Blood. 2016;128:297-312 pubmed 出版商
Martin E, Palmic N, Sanquer S, Lenoir C, Hauck F, Mongellaz C, et al. CTP synthase 1 deficiency in humans reveals its central role in lymphocyte proliferation. Nature. 2014;510:288-92 pubmed 出版商
Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed
产品信息
抗原 :
肿瘤坏死因子甲
Apps. Abbrev. :
流式细胞仪细胞内染色, 流式细胞仪
目录 :
502928
克隆 :
MAb11
产品 :
Alexa Fluor系列荧光染料®700抗人类TNF-α
抗体亚型 :
Mouse IgG1, κ
其他名称 :
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
规格 :
100试验
价格(美元) :
288美元
反应物种 :
人类
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
A700
免疫原 :
E. coli -expressed, recombinant human TNF-α
应用注释 :
ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody. Flow Cytometry 3,5,6,10 : The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations. Additional reported applications (for the relevant formats) include: neutralization 1,2 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 4 and acetone-fixed frozen tissue sections 8 , immunocytochemistry 7 , and immunofluorescence 9 . The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a. Note: For testing human TNF-a in serum or plasma, BioLegend s ELISA Max Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922). The Purified MAb1 antibody is useful in neutralization 2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody. Clone MAb11 cross-reacts to Cat 11
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。