Babco, Signet, Sternberger Monoclonals, Senetek, Covance

抗体

Ultra-LEAF™ Purified anti-human TNF-α

502952

1毫克

615美元

单克隆

小鼠

未共轭

MAb11

人类, 猕猴

酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 中和反应, 流式细胞仪

ELISA, CyTOF®, Flow Cytometry, Intracellular Staining for Flow Cytometry, ICC, Immunofluorescence Microscopy, IHC, Neut

502952

MAb11

Ultra-LEAF™ Purified anti-human TNF-α

Mouse IgG1, κ

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2

1毫克

615美元

Human, Cat (Feline), Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Swine (Pig, Porcine), Pigtailed Macaque, Sooty Mangabey

单克隆

小鼠

ULEAF

E. coli -expressed, recombinant human TNF-α

ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody. Flow Cytometry 3,5,6,10 : The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-α-producing cells within mixed cell populations. Additional reported applications (for the relevant formats) include: neutralization 1,2 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 4 and acetone-fixed frozen tissue sections 8 , immunocytochemistry 7 , and immunofluorescence 9 . The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-α. Note: For testing human TNF-α in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human TNF-α bioactivity (Cat. No. 502951 502952). The Purified MAb1 antibody is useful in neutralization 2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. Nos. 502904 502914) as the detecting antibody.