产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
亮紫色650™抗小鼠TNF-α
目录 :
506333
规格 :
50微克
价格 :
273美元
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
BV650
克隆名称 :
MP6-XT22
反应物种 :
小鼠
应用 :
流式细胞仪
更多信息或购买 :
文章摘录数: 14
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 小鼠
Hering L, Katkeviciute E, Schwarzfischer M, Niechcial A, Riggs J, Wawrzyniak M, et al. Macrophages Compensate for Loss of Protein Tyrosine Phosphatase N2 in Dendritic Cells to Protect from Elevated Colitis. Int J Mol Sci. 2021;22: pubmed 出版商
  • 流式细胞仪; 小鼠; 1:100; 图 4
Shi L, Fu T, Guan B, Chen J, Blando J, Allison J, et al. Interdependent IL-7 and IFN-? signalling in T-cell controls tumour eradication by combined ?-CTLA-4+?-PD-1 therapy. Nat Commun. 2016;7:12335 pubmed 出版商
Palathingal Bava E, George J, Tarique M, Iyer S, Sahay P, Gomez Aguilar B, et al. Pirfenidone increases IL-10 and improves acute pancreatitis in multiple clinically relevant murine models. JCI Insight. 2022;7: pubmed 出版商
MARCHELLETTA R, Krishnan M, Spalinger M, Placone T, Alvarez R, Sayoc Becerra A, et al. T cell protein tyrosine phosphatase protects intestinal barrier function by restricting epithelial tight junction remodeling. J Clin Invest. 2021;131: pubmed 出版商
Rodriguez Garcia A, Lynn R, Poussin M, Eiva M, Shaw L, O Connor R, et al. CAR-T cell-mediated depletion of immunosuppressive tumor-associated macrophages promotes endogenous antitumor immunity and augments adoptive immunotherapy. Nat Commun. 2021;12:877 pubmed 出版商
Hering L, Katkeviciute E, Schwarzfischer M, Busenhart P, Gottier C, Mrdjen D, et al. Protein Tyrosine Phosphatase Non-Receptor Type 2 Function in Dendritic Cells Is Crucial to Maintain Tissue Tolerance. Front Immunol. 2020;11:1856 pubmed 出版商
Shakya S, Asosingh K, Mack J, Maytin E. Optimized protocol for the preparation of single cells from cutaneous wounds for flow cytometric cell sorting and analysis of macrophages. MethodsX. 2020;7:101027 pubmed 出版商
Kumar R, Bunn P, Singh S, Ng S, Montes De Oca M, de Labastida Rivera F, et al. Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis. Cell Rep. 2020;30:2512-2525.e9 pubmed 出版商
Rosenbaum S, Knecht M, Mollaee M, Zhong Z, Erkes D, McCue P, et al. FOXD3 Regulates VISTA Expression in Melanoma. Cell Rep. 2020;30:510-524.e6 pubmed 出版商
Sharma N, Gupta V, Garrido V, Hadad R, Durden B, Kesh K, et al. Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy. J Clin Invest. 2020;130:451-465 pubmed 出版商
Jablonski K, Amici S, Webb L, Ruiz Rosado J, Popovich P, Partida Sanchez S, et al. Novel Markers to Delineate Murine M1 and M2 Macrophages. PLoS ONE. 2015;10:e0145342 pubmed 出版商
Carlson M, West M, COGHILL J, Panoskaltsis Mortari A, Blazar B, Serody J. In vitro-differentiated TH17 cells mediate lethal acute graft-versus-host disease with severe cutaneous and pulmonary pathologic manifestations. Blood. 2009;113:1365-74 pubmed 出版商
Wu S, Ren J. Benfotiamine alleviates diabetes-induced cerebral oxidative damage independent of advanced glycation end-product, tissue factor and TNF-alpha. Neurosci Lett. 2006;394:158-62 pubmed
Patole P, Zecher D, Pawar R, Grone H, Schlondorff D, Anders H. G-rich DNA suppresses systemic lupus. J Am Soc Nephrol. 2005;16:3273-80 pubmed
产品信息
抗原 :
肿瘤坏死因子甲
Apps. Abbrev. :
流式细胞仪细胞内染色
目录 :
506333
克隆 :
MP6-XT22
产品 :
亮紫色650™抗小鼠TNF-α
抗体亚型 :
Rat IgG1, κ
其他名称 :
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha
规格 :
50微克
价格(美元) :
273美元
反应物种 :
小鼠
克隆性 :
单克隆
宿主 :
大鼠
共轭标签/标签/标记 :
BV650
免疫原 :
E. coli -expressed, recombinant mouse TNF-α
应用注释 :
ELISA or ELISPOT Detection : The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 510804) as the capture antibody. ELISA Capture : The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF- (Cat. No. 575209) as the standard. Flow Cytometry 6,11,12 :The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations. Neutralization 1,5,10,16,17 :The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF- . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse TNF- bioactivity in vivo and in vitro (Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include : Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 7-9 in vivo detection 5 , immunofluorescence, and immunocytochemistry. Note : For testing mouse TNF- in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430901) are specially developed and recommended.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。