Babco, Signet, Sternberger Monoclonals, Senetek, Covance

抗体

LEAF™纯化抗人类IFN-γ

507513

500微克

320美元

单克隆

小鼠

未共轭

MD-1

人类, 猕猴

免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片, 免疫组化-冰冻切片

ELISA捕获, 流式细胞仪细胞内染色, 免疫组化-F, 西方墨点法

507513

MD-1

LEAF™纯化抗人类IFN-γ

Mouse IgG1, κ

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma

500微克

320美元

Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus

单克隆

小鼠

LEAF

ELISA 1,2,5 and ELISPOT 3,4 Capture: The Purified MD-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4S.B3 (Cat. No. 502504/502514) antibody as the detecting antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. The LEAF™ Purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5-2 µg/ml is recommended. Neutralization 1 : The LEAF™ Purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IFN-γ bioactivity (Cat. No. 507513). Additional reported applications (for the relevant formats) include: Western blotting 1,2 , intracellular staining for flow cytometry 6 , and immunohistochemical staining 7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections. Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.