Babco, Signet, Sternberger Monoclonals, Senetek, Covance

抗体

纯化抗哺乳动物雷帕霉素靶蛋白磷酸化(Ser2448)

610301

25微克

112美元

单克隆

小鼠

未共轭

A17024A

人类, 小鼠

免疫印迹, 免疫细胞化学

西方墨点法, 免疫细胞化学

610301

A17024A

纯化抗哺乳动物雷帕霉素靶蛋白磷酸化(Ser2448)

Mouse IgG1, κ

Mammalian target of rapamycin, FRAP, RAFT

25微克

112美元

人类, 小鼠

单克隆

小鼠

PURE

Based off complete sequence homology of the immunizing peptide, this clone is predicted to recognize both mouse and rat mTOR phosphorylated at serine 2448. NIH/3T3 cells (murine origin) used in this experiment contain less mTOR than the human-derived HeLa cells (see pan mTOR blot). Thus, the reduced signal observed in these lysates is due to less total mTOR and not a decreased affinity of this clone for mouse mTOR phosphor (Ser2448).