产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
异硫氰酸荧光素抗H2A.X磷酸化(Ser139)
目录 :
613403
规格 :
25试验
价格 :
165美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
异硫氰酸荧光素
抗原修饰 :
磷酸化
克隆名称 :
2F3
反应物种 :
人类, 小鼠
应用 :
流式细胞仪
更多信息或购买 :
文章摘录数: 36
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 小鼠; 图 4d
Mitroulis I, Ruppova K, Wang B, Chen L, Grzybek M, Grinenko T, et al. Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity. Cell. 2018;172:147-161.e12 pubmed 出版商
Chang Y, Ignatova D, Hoetzenecker W, Pascolo S, Fassnacht C, Guenova E. Increased Chlormethine-Induced DNA Double-Stranded Breaks in Malignant T Cells from Mycosis Fungoides Skin Lesions. JID Innov. 2022;2:100069 pubmed 出版商
Young K, Eudy E, Bell R, Loberg M, Stearns T, Sharma D, et al. Decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging. Cell Stem Cell. 2021;28:1473-1482.e7 pubmed 出版商
Sarkar A, Gandhi V. Activation of ATM kinase by ROS generated during ionophore-induced mitophagy in human T and B cell malignancies. Mol Cell Biochem. 2021;476:417-423 pubmed 出版商
Chen Z, Chen C, Zhou T, Duan C, Wang Q, Zhou X, et al. A high-throughput drug combination screen identifies an anti-glioma synergism between TH588 and PI3K inhibitors. Cancer Cell Int. 2020;20:337 pubmed 出版商
Jiao S, Subudhi S, Aparicio A, Ge Z, Guan B, Miura Y, et al. Differences in Tumor Microenvironment Dictate T Helper Lineage Polarization and Response to Immune Checkpoint Therapy. Cell. 2019;179:1177-1190.e13 pubmed 出版商
Shibamoto M, Higo T, Naito A, Nakagawa A, Sumida T, Okada K, et al. Activation of DNA Damage Response and Cellular Senescence in Cardiac Fibroblasts Limit Cardiac Fibrosis After Myocardial Infarction. Int Heart J. 2019;60:944-957 pubmed 出版商
Zuazo M, Arasanz H, Fernández Hinojal G, García Granda M, Gato M, Bocanegra A, et al. Functional systemic CD4 immunity is required for clinical responses to PD-L1/PD-1 blockade therapy. EMBO Mol Med. 2019;: pubmed 出版商
Han Y, Chen M, Wang H, Hsu J, Li C, Chu Y, et al. Synergism of PARP inhibitor fluzoparib (HS10160) and MET inhibitor HS10241 in breast and ovarian cancer cells. Am J Cancer Res. 2019;9:608-618 pubmed
Chen J, Li N, Yin Y, Zheng N, Min M, Lin B, et al. Methyltransferase Nsd2 Ensures Germinal Center Selection by Promoting Adhesive Interactions between B Cells and Follicular Dendritic Cells. Cell Rep. 2018;25:3393-3404.e6 pubmed 出版商
Caruso L, Martin K, Lauretti E, Hulse M, Siciliano M, Lupey Green L, et al. Poly(ADP-ribose) Polymerase 1, PARP1, modifies EZH2 and inhibits EZH2 histone methyltransferase activity after DNA damage. Oncotarget. 2018;9:10585-10605 pubmed 出版商
Scheffel M, Scurti G, Simms P, Garrett Mayer E, Mehrotra S, Nishimura M, et al. Efficacy of Adoptive T-cell Therapy Is Improved by Treatment with the Antioxidant N-Acetyl Cysteine, Which Limits Activation-Induced T-cell Death. Cancer Res. 2016;76:6006-6016 pubmed
Namas R, Renauer P, Ognenovski M, Tsou P, Sawalha A. Histone H2AX phosphorylation as a measure of DNA double-strand breaks and a marker of environmental stress and disease activity in lupus. Lupus Sci Med. 2016;3:e000148 pubmed 出版商
Maya Mendoza A, Ostrakova J, Kosar M, Hall A, Duskova P, Mistrik M, et al. Myc and Ras oncogenes engage different energy metabolism programs and evoke distinct patterns of oxidative and DNA replication stress. Mol Oncol. 2015;9:601-16 pubmed 出版商
Horrell S, Chalker D. LIA4 encodes a chromoshadow domain protein required for genomewide DNA rearrangements in Tetrahymena thermophila. Eukaryot Cell. 2014;13:1300-11 pubmed 出版商
Zhdanova N, Draskovic I, Minina J, Karamysheva T, Novo C, Liu W, et al. Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells. Mol Cell Biol. 2014;34:2786-99 pubmed 出版商
Crown K, Savytskyy O, Malik S, LOGSDON J, Williams R, Tainer J, et al. A mutation in the FHA domain of Coprinus cinereus Nbs1 Leads to Spo11-independent meiotic recombination and chromosome segregation. G3 (Bethesda). 2013;3:1927-43 pubmed 出版商
Jha H, Upadhyay S, A J Prasad M, Lu J, Cai Q, Saha A, et al. H2AX phosphorylation is important for LANA-mediated Kaposi's sarcoma-associated herpesvirus episome persistence. J Virol. 2013;87:5255-69 pubmed 出版商
Titus S, Li F, Stobezki R, Akula K, Unsal E, Jeong K, et al. Impairment of BRCA1-related DNA double-strand break repair leads to ovarian aging in mice and humans. Sci Transl Med. 2013;5:172ra21 pubmed 出版商
Schenkwein D, Turkki V, Ahlroth M, Timonen O, Airenne K, Yla Herttuala S. rDNA-directed integration by an HIV-1 integrase--I-PpoI fusion protein. Nucleic Acids Res. 2013;41:e61 pubmed 出版商
Kuefner M, Brand M, Ehrlich J, Braga L, Uder M, Semelka R. Effect of antioxidants on X-ray-induced ?-H2AX foci in human blood lymphocytes: preliminary observations. Radiology. 2012;264:59-67 pubmed 出版商
Yoshihara Y, Wu D, Kubo N, Sang M, Nakagawara A, Ozaki T. Inhibitory role of E2F-1 in the regulation of tumor suppressor p53 during DNA damage response. Biochem Biophys Res Commun. 2012;421:57-63 pubmed 出版商
Smart D, Lynch A. Evaluating the genotoxicity of topoisomerase-targeted antibiotics. Mutagenesis. 2012;27:359-65 pubmed 出版商
Zhang M, Kleber S, Röhrich M, Timke C, Han N, Tuettenberg J, et al. Blockade of TGF-? signaling by the TGF?R-I kinase inhibitor LY2109761 enhances radiation response and prolongs survival in glioblastoma. Cancer Res. 2011;71:7155-67 pubmed 出版商
Smart D, Ahmedi K, Harvey J, Lynch A. Genotoxicity screening via the ?H2AX by flow assay. Mutat Res. 2011;715:25-31 pubmed 出版商
Bu Y, Suenaga Y, Okoshi R, Sang M, Kubo N, Song F, et al. NFBD1/MDC1 participates in the regulation of G2/M transition in mammalian cells. Biochem Biophys Res Commun. 2010;397:157-62 pubmed 出版商
Lukaszewicz A, Howard Till R, Novatchkova M, Mochizuki K, Loidl J. MRE11 and COM1/SAE2 are required for double-strand break repair and efficient chromosome pairing during meiosis of the protist Tetrahymena. Chromosoma. 2010;119:505-18 pubmed 出版商
Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S. A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks. Nucleic Acids Res. 2010;38:e129 pubmed 出版商
Yamada C, Ozaki T, Ando K, Suenaga Y, Inoue K, Ito Y, et al. RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at Ser-15 and acts as a co-activator for p53. J Biol Chem. 2010;285:16693-703 pubmed 出版商
Massignan T, Stewart R, Biasini E, Solomon I, Bonetto V, Chiesa R, et al. A novel, drug-based, cellular assay for the activity of neurotoxic mutants of the prion protein. J Biol Chem. 2010;285:7752-65 pubmed 出版商
Beels L, Bacher K, De Wolf D, Werbrouck J, Thierens H. gamma-H2AX foci as a biomarker for patient X-ray exposure in pediatric cardiac catheterization: are we underestimating radiation risks?. Circulation. 2009;120:1903-9 pubmed 出版商
Loidl J, Mochizuki K. Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage. Mol Biol Cell. 2009;20:2428-37 pubmed 出版商
Akbay E, Contreras C, Perera S, Sullivan J, Broaddus R, Schorge J, et al. Differential roles of telomere attrition in type I and II endometrial carcinogenesis. Am J Pathol. 2008;173:536-44 pubmed 出版商
Mochizuki K, Novatchkova M, Loidl J. DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena. J Cell Sci. 2008;121:2148-58 pubmed 出版商
Rossi D, Bryder D, Seita J, Nussenzweig A, Hoeijmakers J, Weissman I. Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Nature. 2007;447:725-9 pubmed
Xiao R, Sun Y, Ding J, Lin S, Rose D, Rosenfeld M, et al. Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Mol Cell Biol. 2007;27:5393-402 pubmed
产品信息
抗原 :
H2A.X磷酸化Ser139
Apps. Abbrev. :
流式细胞仪细胞内染色
目录 :
613403
克隆 :
2F3
产品 :
异硫氰酸荧光素抗H2A.X磷酸化(Ser139)
抗体亚型 :
Mouse IgG1, κ
其他名称 :
H2A.x, H2a/x, 组蛋白2A, 组蛋白2A.X, 丙H2A组蛋白家族成员X
规格 :
25试验
价格(美元) :
165美元
反应物种 :
人类, 小鼠
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
异硫氰酸荧光素
免疫原 :
Modified peptide
应用注释 :
Additional reported applications (for the relevant formats of this clone) include: immunohistochemistry on paraffin embedded sections 2 , immunofluorescence microscopy 3-9 , Western blotting 10-12 , and flow cytometry 1,13 . Clone 2F3 cross-reacts with mouse 4 . Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry 1. Prepare 70% absolute ethanol. Chill solution by storing at -20 C. 2. Prepare cells of interest. 3. Wash 1X with PBS, centrifuge at 350g for 5 min. 4. Discard the supernatant and vortex to loosen cell pellet. 5. Add pre-cooled 70% ethanol drop by drop, while vortexing. 6. Incubate at -20 C for 60 minutes. 7. Wash 3X with BioLegend Cell Staining Buffer and resuspend the cells at 0.5-1 X 10 7 cells/ml in the cell staining buffer. 8. Perform immunofluorescent staining for flow cytometry.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。