产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
Alexa Fluor系列荧光染料®488抗H2A.X磷酸化(Ser139)
目录 :
613406
规格 :
100试验
价格 :
370美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
AF488
抗原修饰 :
磷酸化
克隆名称 :
2F3
反应物种 :
人类, 小鼠
应用 :
流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 37
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类
Warnes G. Flow cytometric detection of hyper-polarized mitochondria in regulated and accidental cell death processes. Apoptosis. 2020;25:548-557 pubmed 出版商
  • 免疫组化-石蜡切片; 小鼠; 1:1000; 图 4b
Okawa A, Morioka T, Imaoka T, Kakinuma S, Matsumoto Y. Differential expression of DNA-dependent protein kinase catalytic subunit in the brain of neonatal mice and young adult mice. Proc Jpn Acad Ser B Phys Biol Sci. 2020;96:171-179 pubmed 出版商
  • 流式细胞仪; 人类; 图 4
Dorn P, Tièche C, Peng R, Froment L, Schmid R, Marti T. Schedule-dependent increased efficiency of pemetrexed-ionizing radiation combination therapy elicits a differential DNA damage response in lung cancer cells. Cancer Cell Int. 2016;16:66 pubmed 出版商
Fabián Morales E, Fernández Cáceres C, Gudiño A, Andonegui Elguera M, Torres Arciga K, Escobar Arrazola M, et al. Genotoxicity of Marijuana in Mono-Users. Front Psychiatry. 2021;12:753562 pubmed 出版商
Jiao S, Subudhi S, Aparicio A, Ge Z, Guan B, Miura Y, et al. Differences in Tumor Microenvironment Dictate T Helper Lineage Polarization and Response to Immune Checkpoint Therapy. Cell. 2019;179:1177-1190.e13 pubmed 出版商
Pond K, de Renty C, Yagle M, Ellis N. Rescue of collapsed replication forks is dependent on NSMCE2 to prevent mitotic DNA damage. PLoS Genet. 2019;15:e1007942 pubmed 出版商
Fichtman B, Zagairy F, Biran N, Barsheshet Y, Chervinsky E, Ben Neriah Z, et al. Combined loss of LAP1B and LAP1C results in an early onset multisystemic nuclear envelopathy. Nat Commun. 2019;10:605 pubmed 出版商
Picard E, Godet Y, Laheurte C, Dosset M, Galaine J, Beziaud L, et al. Circulating NKp46+ Natural Killer cells have a potential regulatory property and predict distinct survival in Non-Small Cell Lung Cancer. Oncoimmunology. 2019;8:e1527498 pubmed 出版商
Prasad C, Prasad S, Yadav S, Pandey L, Singh S, Pradhan S, et al. Olaparib modulates DNA repair efficiency, sensitizes cervical cancer cells to cisplatin and exhibits anti-metastatic property. Sci Rep. 2017;7:12876 pubmed 出版商
Castro M, Carson G, McConnell M, Herst P. High Dose Ascorbate Causes Both Genotoxic and Metabolic Stress in Glioma Cells. Antioxidants (Basel). 2017;6: pubmed 出版商
Kopova I, Lavrentiev V, Vacik J, Bacakova L. Growth and potential damage of human bone-derived cells cultured on fresh and aged C60/Ti films. PLoS ONE. 2015;10:e0123680 pubmed 出版商
Maya Mendoza A, Ostrakova J, Kosar M, Hall A, Duskova P, Mistrik M, et al. Myc and Ras oncogenes engage different energy metabolism programs and evoke distinct patterns of oxidative and DNA replication stress. Mol Oncol. 2015;9:601-16 pubmed 出版商
Horrell S, Chalker D. LIA4 encodes a chromoshadow domain protein required for genomewide DNA rearrangements in Tetrahymena thermophila. Eukaryot Cell. 2014;13:1300-11 pubmed 出版商
Zhdanova N, Draskovic I, Minina J, Karamysheva T, Novo C, Liu W, et al. Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells. Mol Cell Biol. 2014;34:2786-99 pubmed 出版商
Becker M, Schmezer P, Breuer R, Haas S, Essers M, Krammer P, et al. The traditional Chinese medical compound Rocaglamide protects nonmalignant primary cells from DNA damage-induced toxicity by inhibition of p53 expression. Cell Death Dis. 2014;5:e1000 pubmed 出版商
Crown K, Savytskyy O, Malik S, LOGSDON J, Williams R, Tainer J, et al. A mutation in the FHA domain of Coprinus cinereus Nbs1 Leads to Spo11-independent meiotic recombination and chromosome segregation. G3 (Bethesda). 2013;3:1927-43 pubmed 出版商
Furia L, Pelicci P, Faretta M. A computational platform for robotized fluorescence microscopy (II): DNA damage, replication, checkpoint activation, and cell cycle progression by high-content high-resolution multiparameter image-cytometry. Cytometry A. 2013;83:344-55 pubmed 出版商
Jha H, Upadhyay S, A J Prasad M, Lu J, Cai Q, Saha A, et al. H2AX phosphorylation is important for LANA-mediated Kaposi's sarcoma-associated herpesvirus episome persistence. J Virol. 2013;87:5255-69 pubmed 出版商
Titus S, Li F, Stobezki R, Akula K, Unsal E, Jeong K, et al. Impairment of BRCA1-related DNA double-strand break repair leads to ovarian aging in mice and humans. Sci Transl Med. 2013;5:172ra21 pubmed 出版商
Heo J, Cho J, Kim J. HJURP regulates cellular senescence in human fibroblasts and endothelial cells via a p53-dependent pathway. J Gerontol A Biol Sci Med Sci. 2013;68:914-25 pubmed 出版商
Schenkwein D, Turkki V, Ahlroth M, Timonen O, Airenne K, Yla Herttuala S. rDNA-directed integration by an HIV-1 integrase--I-PpoI fusion protein. Nucleic Acids Res. 2013;41:e61 pubmed 出版商
Kuefner M, Brand M, Ehrlich J, Braga L, Uder M, Semelka R. Effect of antioxidants on X-ray-induced ?-H2AX foci in human blood lymphocytes: preliminary observations. Radiology. 2012;264:59-67 pubmed 出版商
Yoshihara Y, Wu D, Kubo N, Sang M, Nakagawara A, Ozaki T. Inhibitory role of E2F-1 in the regulation of tumor suppressor p53 during DNA damage response. Biochem Biophys Res Commun. 2012;421:57-63 pubmed 出版商
Zhang M, Kleber S, Röhrich M, Timke C, Han N, Tuettenberg J, et al. Blockade of TGF-? signaling by the TGF?R-I kinase inhibitor LY2109761 enhances radiation response and prolongs survival in glioblastoma. Cancer Res. 2011;71:7155-67 pubmed 出版商
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Bu Y, Suenaga Y, Okoshi R, Sang M, Kubo N, Song F, et al. NFBD1/MDC1 participates in the regulation of G2/M transition in mammalian cells. Biochem Biophys Res Commun. 2010;397:157-62 pubmed 出版商
Lukaszewicz A, Howard Till R, Novatchkova M, Mochizuki K, Loidl J. MRE11 and COM1/SAE2 are required for double-strand break repair and efficient chromosome pairing during meiosis of the protist Tetrahymena. Chromosoma. 2010;119:505-18 pubmed 出版商
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Leone S, Cornetta T, Basso E, Cozzi R. Resveratrol induces DNA double-strand breaks through human topoisomerase II interaction. Cancer Lett. 2010;295:167-72 pubmed 出版商
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Akbay E, Contreras C, Perera S, Sullivan J, Broaddus R, Schorge J, et al. Differential roles of telomere attrition in type I and II endometrial carcinogenesis. Am J Pathol. 2008;173:536-44 pubmed 出版商
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产品信息
抗原 :
H2A.X磷酸化Ser139
Apps. Abbrev. :
流式细胞仪细胞内染色
目录 :
613406
克隆 :
2F3
产品 :
Alexa Fluor系列荧光染料®488抗H2A.X磷酸化(Ser139)
抗体亚型 :
Mouse IgG1, κ
其他名称 :
H2A.x, H2a/x, 组蛋白2A, 组蛋白2A.X, 丙H2A组蛋白家族成员X
规格 :
100试验
价格(美元) :
370美元
反应物种 :
人类, 小鼠
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
A488
免疫原 :
Modified peptide
应用注释 :
Additional reported applications (for the relevant formats of this clone) include: immunohistochemistry on paraffin embedded sections 2 , immunofluorescence microscopy 3-9 , Western blotting 10-12 , and flow cytometry 1,13 . Clone 2F3 cross-reacts with mouse 4 . Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry 1. Prepare 70% absolute ethanol. Chill solution by storing at -20 C. 2. Prepare cells of interest. 3. Wash 1X with PBS, centrifuge at 350g for 5 min. 4. Discard the supernatant and vortex to loosen cell pellet. 5. Add pre-cooled 70% ethanol drop by drop, while vortexing. 6. Incubate at -20 C for 60 minutes. 7. Wash 3X with BioLegend Cell Staining Buffer and resuspend the cells at 0.5-1 X 10 7 cells/ml in the cell staining buffer. 8. Perform immunofluorescent staining for flow cytometry.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。