产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
Alexa Fluor系列荧光染料®647抗H2A.X磷酸化(Ser139)
目录 :
613407
规格 :
25试验
价格 :
188美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
AF647
抗原修饰 :
磷酸化
克隆名称 :
2F3
反应物种 :
人类, 小鼠
应用 :
免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 37
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类
Warnes G. Flow cytometric detection of hyper-polarized mitochondria in regulated and accidental cell death processes. Apoptosis. 2020;25:548-557 pubmed 出版商
  • 免疫组化-石蜡切片; 小鼠; 1:1000; 图 4b
Okawa A, Morioka T, Imaoka T, Kakinuma S, Matsumoto Y. Differential expression of DNA-dependent protein kinase catalytic subunit in the brain of neonatal mice and young adult mice. Proc Jpn Acad Ser B Phys Biol Sci. 2020;96:171-179 pubmed 出版商
  • 免疫组化-石蜡切片; 人类
  • 免疫组化; 人类
Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed 出版商
  • 免疫细胞化学; 小鼠; 图 3a
Yoon Y, Storm K, Kamimae Lanning A, Goloviznina N, Kurre P. Endogenous DNA Damage Leads to p53-Independent Deficits in Replicative Fitness in Fetal Murine Fancd2-/- Hematopoietic Stem and Progenitor Cells. Stem Cell Reports. 2016;7:840-853 pubmed 出版商
Rivas M, Durmaz C, Kloetgen A, Chin C, Chen Z, Bhinder B, et al. Cohesin Core Complex Gene Dosage Contributes to Germinal Center Derived Lymphoma Phenotypes and Outcomes. Front Immunol. 2021;12:688493 pubmed 出版商
Chen R, Okeyo Owuor T, Patel R, Casey E, Cluster A, Yang W, et al. Kmt2c mutations enhance HSC self-renewal capacity and convey a selective advantage after chemotherapy. Cell Rep. 2021;34:108751 pubmed 出版商
Tamayo Orrego L, Gallo D, Racicot F, Bemmo A, Mohan S, Ho B, et al. Sonic hedgehog accelerates DNA replication to cause replication stress promoting cancer initiation in medulloblastoma. Nat Cancer. 2020;1:840-854 pubmed 出版商
Atashpaz S, Samadi Shams S, Gonzalez J, Sebestyén E, Arghavanifard N, Gnocchi A, et al. ATR expands embryonic stem cell fate potential in response to replication stress. elife. 2020;9: pubmed 出版商
de Jong M, Langendonk M, Reitsma B, Herbers P, Lodewijk M, Nijland M, et al. WEE1 inhibition synergizes with CHOP chemotherapy and radiation therapy through induction of premature mitotic entry and DNA damage in diffuse large B-cell lymphoma. Ther Adv Hematol. 2020;11:2040620719898373 pubmed 出版商
de Jong M, Langendonk M, Reitsma B, Herbers P, Nijland M, Huls G, et al. WEE1 Inhibition Enhances Anti-Apoptotic Dependency as a Result of Premature Mitotic Entry and DNA Damage. Cancers (Basel). 2019;11: pubmed 出版商
Wang T, Fan C, Yao A, Xu X, Zheng G, You Y, et al. The Adaptor Protein CARD9 Protects against Colon Cancer by Restricting Mycobiota-Mediated Expansion of Myeloid-Derived Suppressor Cells. Immunity. 2018;49:504-514.e4 pubmed 出版商
Lin J, Fallahi Sichani M, Sorger P. Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method. Nat Commun. 2015;6:8390 pubmed 出版商
Kang J, Lienhard M, Pastor W, Chawla A, Novotny M, Tsagaratou A, et al. Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis. Proc Natl Acad Sci U S A. 2015;112:E4236-45 pubmed 出版商
Tierny D, Serres F, Segaoula Z, Bemelmans I, Bouchaert E, Pétain A, et al. Phase I Clinical Pharmacology Study of F14512, a New Polyamine-Vectorized Anticancer Drug, in Naturally Occurring Canine Lymphoma. Clin Cancer Res. 2015;21:5314-23 pubmed 出版商
Maya Mendoza A, Ostrakova J, Kosar M, Hall A, Duskova P, Mistrik M, et al. Myc and Ras oncogenes engage different energy metabolism programs and evoke distinct patterns of oxidative and DNA replication stress. Mol Oncol. 2015;9:601-16 pubmed 出版商
Horrell S, Chalker D. LIA4 encodes a chromoshadow domain protein required for genomewide DNA rearrangements in Tetrahymena thermophila. Eukaryot Cell. 2014;13:1300-11 pubmed 出版商
Zhdanova N, Draskovic I, Minina J, Karamysheva T, Novo C, Liu W, et al. Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells. Mol Cell Biol. 2014;34:2786-99 pubmed 出版商
Crown K, Savytskyy O, Malik S, LOGSDON J, Williams R, Tainer J, et al. A mutation in the FHA domain of Coprinus cinereus Nbs1 Leads to Spo11-independent meiotic recombination and chromosome segregation. G3 (Bethesda). 2013;3:1927-43 pubmed 出版商
Jha H, Upadhyay S, A J Prasad M, Lu J, Cai Q, Saha A, et al. H2AX phosphorylation is important for LANA-mediated Kaposi's sarcoma-associated herpesvirus episome persistence. J Virol. 2013;87:5255-69 pubmed 出版商
Titus S, Li F, Stobezki R, Akula K, Unsal E, Jeong K, et al. Impairment of BRCA1-related DNA double-strand break repair leads to ovarian aging in mice and humans. Sci Transl Med. 2013;5:172ra21 pubmed 出版商
Schenkwein D, Turkki V, Ahlroth M, Timonen O, Airenne K, Yla Herttuala S. rDNA-directed integration by an HIV-1 integrase--I-PpoI fusion protein. Nucleic Acids Res. 2013;41:e61 pubmed 出版商
Kuefner M, Brand M, Ehrlich J, Braga L, Uder M, Semelka R. Effect of antioxidants on X-ray-induced ?-H2AX foci in human blood lymphocytes: preliminary observations. Radiology. 2012;264:59-67 pubmed 出版商
Yoshihara Y, Wu D, Kubo N, Sang M, Nakagawara A, Ozaki T. Inhibitory role of E2F-1 in the regulation of tumor suppressor p53 during DNA damage response. Biochem Biophys Res Commun. 2012;421:57-63 pubmed 出版商
Zhang M, Kleber S, Röhrich M, Timke C, Han N, Tuettenberg J, et al. Blockade of TGF-? signaling by the TGF?R-I kinase inhibitor LY2109761 enhances radiation response and prolongs survival in glioblastoma. Cancer Res. 2011;71:7155-67 pubmed 出版商
Brel V, Annereau J, Vispé S, Kruczynski A, Bailly C, Guilbaud N. Cytotoxicity and cell death mechanisms induced by the polyamine-vectorized anti-cancer drug F14512 targeting topoisomerase II. Biochem Pharmacol. 2011;82:1843-52 pubmed 出版商
Bu Y, Suenaga Y, Okoshi R, Sang M, Kubo N, Song F, et al. NFBD1/MDC1 participates in the regulation of G2/M transition in mammalian cells. Biochem Biophys Res Commun. 2010;397:157-62 pubmed 出版商
Lukaszewicz A, Howard Till R, Novatchkova M, Mochizuki K, Loidl J. MRE11 and COM1/SAE2 are required for double-strand break repair and efficient chromosome pairing during meiosis of the protist Tetrahymena. Chromosoma. 2010;119:505-18 pubmed 出版商
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Ouyang K, Woo L, Zhu J, Huo D, Matunis M, Ellis N. SUMO modification regulates BLM and RAD51 interaction at damaged replication forks. PLoS Biol. 2009;7:e1000252 pubmed 出版商
Massignan T, Stewart R, Biasini E, Solomon I, Bonetto V, Chiesa R, et al. A novel, drug-based, cellular assay for the activity of neurotoxic mutants of the prion protein. J Biol Chem. 2010;285:7752-65 pubmed 出版商
Beels L, Bacher K, De Wolf D, Werbrouck J, Thierens H. gamma-H2AX foci as a biomarker for patient X-ray exposure in pediatric cardiac catheterization: are we underestimating radiation risks?. Circulation. 2009;120:1903-9 pubmed 出版商
Loidl J, Mochizuki K. Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage. Mol Biol Cell. 2009;20:2428-37 pubmed 出版商
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产品信息
抗原 :
H2A.X磷酸化Ser139
Apps. Abbrev. :
流式细胞仪细胞内染色
目录 :
613407
克隆 :
2F3
产品 :
Alexa Fluor系列荧光染料®647抗H2A.X磷酸化(Ser139)
抗体亚型 :
Mouse IgG1, κ
其他名称 :
H2A.x, H2a/x, 组蛋白2A, 组蛋白2A.X, 丙H2A组蛋白家族成员X
规格 :
25试验
价格(美元) :
188美元
反应物种 :
人类, 小鼠
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
A647
免疫原 :
Modified peptide
应用注释 :
Additional reported applications (for the relevant formats of this clone) include: immunohistochemistry on paraffin embedded sections 2 , immunofluorescence microscopy 3-9 , Western blotting 10-12 , and flow cytometry 1,13 . Clone 2F3 cross-reacts with mouse 4 . Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry 1. Prepare 70% absolute ethanol. Chill solution by storing at -20 C. 2. Prepare cells of interest. 3. Wash 1X with PBS, centrifuge at 350g for 5 min. 4. Discard the supernatant and vortex to loosen cell pellet. 5. Add pre-cooled 70% ethanol drop by drop, while vortexing. 6. Incubate at -20 C for 60 minutes. 7. Wash 3X with BioLegend Cell Staining Buffer and resuspend the cells at 0.5-1 X 10 7 cells/ml in the cell staining buffer. 8. Perform immunofluorescent staining for flow cytometry.
更多信息或购买 :
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品,旨在加速生命科学领域的研发工作。