产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
Alexa Fluor系列荧光染料®488抗NFATc1
目录 :
649603
规格 :
25微克
价格 :
147美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
AF488
克隆名称 :
7A6
反应物种 :
人类, 小鼠, 大鼠
应用 :
流式细胞仪
更多信息或购买 :
BioLegend 产品网页
文章摘录数: 9
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 小鼠; 1:100; 图 4c
Leclerc M, Voilin E, Gros G, Corgnac S, de Montpreville V, Validire P, et al. Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1. Nat Commun. 2019;10:3345 pubmed 出版商
Trefzer A, Kadam P, Wang S, Pennavaria S, Lober B, Akçabozan B, et al. Dynamic adoption of anergy by antigen-exhausted CD4+ T cells. Cell Rep. 2021;34:108748 pubmed 出版商
Morelli A, Sumpter T, Rojas Canales D, Bandyopadhyay M, Chen Z, Tkacheva O, et al. Neurokinin-1 Receptor Signaling Is Required for Efficient Ca2+ Flux in T-Cell-Receptor-Activated T Cells. Cell Rep. 2020;30:3448-3465.e8 pubmed 出版商
Kannan S, Aitken M, Herbrich S, Golfman L, Hall M, Mak D, et al. Anti-Leukemia Effects of Notch-Mediated Inhibition of Oncogenic PLK1 in B-Cell Acute Lymphoblastic Leukemia. Mol Cancer Ther. 2019;: pubmed 出版商
Nonomura K, Lukacs V, Sweet D, Goddard L, Kanie A, Whitwam T, et al. Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation. Proc Natl Acad Sci U S A. 2018;115:12817-12822 pubmed 出版商
Cuadrado E, van den Biggelaar M, de Kivit S, Chen Y, Slot M, Doubal I, et al. Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity. Immunity. 2018;48:1046-1059.e6 pubmed 出版商
Guichard V, Bonilla N, Durand A, Audemard Verger A, Guilbert T, Martin B, et al. Calcium-mediated shaping of naive CD4 T-cell phenotype and function. elife. 2017;6: pubmed 出版商
Hoffmann U, Neudörfl C, Daemen K, Keil J, Stevanovic Meyer M, Lehner F, et al. NK Cells of Kidney Transplant Recipients Display an Activated Phenotype that Is Influenced by Immunosuppression and Pathological Staging. PLoS ONE. 2015;10:e0132484 pubmed 出版商
Fan W, Liang D, Tang Y, Qu B, Cui H, Luo X, et al. Identification of microRNA-31 as a novel regulator contributing to impaired interleukin-2 production in T cells from patients with systemic lupus erythematosus. Arthritis Rheum. 2012;64:3715-25 pubmed 出版商
产品信息
抗原 :
NFATc1
Apps. Abbrev. :
流式细胞仪细胞内染色
目录 :
649603
克隆 :
7A6
产品 :
Alexa Fluor系列荧光染料®488抗NFATc1
抗体亚型 :
Mouse IgG1, κ
其他名称 :
Nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, NFAT2, NFATc
规格 :
25微克
价格(美元) :
147美元
反应物种 :
人类, 小鼠, 大鼠
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
A488
免疫原 :
重组蛋白人的NFATc1氨基酸197-304
应用注释 :
7A6 antibody detects endogenous human NFATC1 in Western blot with an approximate molecular weight of 100 - 120 kD (different isoforms) (Cat. No. 649602). The optimal concentration should be determined by titration for each individual assay of interest. . . Clone 7A6 Intracellular Staining Procedure: Prepare the fix/perm solution, consisting of 1% paraformaldehyde in 70% ethanol. Chill for at least one hour at -20 C. Suspend cells in cell staining buffer and perform surface stain if necessary. Centrifuge for 5 minutes at 1500 RPM and discard supernatant. Resuspend cells in 500 uL of fix/perm solution for up to 2 x 10^6 cells and gently vortex. Incubate cells on ice for 30-60 minutes in the dark. Add 2 mL PBS and centrifuge cells for 1500 RPM for 5 minutes. Wash cells 1X with 2 mL cell staining buffer, centrifuge at 1500 RPM for 5 minutes, discard supernatant. Adjust cell concentration to about 1 x 10^7 cells/mL, add 100 uL (about 1 x 10^6 cells) to a FACS tube that already contains the staining antibody and mix gently. Incubate 15 minutes at room temperature. Wash 2X with cell staining buffer as in step 6. Resuspend cells in cell staining buffer and analyze.
更多信息或购买 :
BioLegend 产品网页
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品，旨在加速生命科学领域的研发工作。