产品简要
公司名称 :
BioLegend
其他品牌 :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
产品类型 :
抗体
产品名称 :
纯化抗RNA聚合酶II RPB1
目录 :
920204
规格 :
100微克
价格 :
253美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
H5
反应物种 :
人类, 小鼠, 大鼠, brewer's yeast, fruit fly , roundworm
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, ChIP-Seq
更多信息或购买 :
BioLegend 产品网页
文章摘录数: 20
出版应用/物种/样本/稀释参考文献
  • 免疫组化; fruit fly ; 1:20; 图 2c
Kuhn T, Pascual García P, Gozalo A, Little S, Capelson M. Chromatin targeting of nuclear pore proteins induces chromatin decondensation. J Cell Biol. 2019;218:2945-2961 pubmed 出版商
  • 免疫组化; 小鼠; 1:50; 图 2g
Nagaraj R, Sharpley M, Chi F, Braas D, Zhou Y, Kim R, et al. Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation. Cell. 2017;168:210-223.e11 pubmed 出版商
  • 免疫组化; roundworm ; 1:500; 图 1a
  • 免疫印迹; roundworm ; 1:500; 图 3
Ahn J, Rechsteiner A, Strome S, Kelly W. A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans. PLoS Genet. 2016;12:e1006227 pubmed 出版商
  • 免疫印迹; brewer's yeast; 图 3C
Sathianathan A, Ravichandran P, Lippi J, Cohen L, Messina A, Shaju S, et al. The Eaf3/5/7 Subcomplex Stimulates NuA4 Interaction with Methylated Histone H3 Lys-36 and RNA Polymerase II. J Biol Chem. 2016;291:21195-21207 pubmed
  • ChIP-Seq; 小鼠; 图 s2
Masuda A, Takeda J, Okuno T, Okamoto T, Ohkawara B, Ito M, et al. Position-specific binding of FUS to nascent RNA regulates mRNA length. Genes Dev. 2015;29:1045-57 pubmed 出版商
  • 免疫印迹; 人类; 图 2
Liang K, Gao X, Gilmore J, Florens L, Washburn M, Smith E, et al. Characterization of human cyclin-dependent kinase 12 (CDK12) and CDK13 complexes in C-terminal domain phosphorylation, gene transcription, and RNA processing. Mol Cell Biol. 2015;35:928-38 pubmed 出版商
Chappidi N, Nascakova Z, Boleslavska B, Zellweger R, Isik E, Andrs M, et al. Fork Cleavage-Religation Cycle and Active Transcription Mediate Replication Restart after Fork Stalling at Co-transcriptional R-Loops. Mol Cell. 2019;: pubmed 出版商
Zhou K, Shi H, Lyu R, Wylder A, Matuszek Ż, Pan J, et al. Regulation of Co-transcriptional Pre-mRNA Splicing by m6A through the Low-Complexity Protein hnRNPG. Mol Cell. 2019;76:70-81.e9 pubmed 出版商
Ryu H, Su D, Wilson Eisele N, Zhao D, Lopez Giraldez F, Hochstrasser M. The Ulp2 SUMO protease promotes transcription elongation through regulation of histone sumoylation. EMBO J. 2019;38:e102003 pubmed 出版商
Tolza C, Bejjani F, Evanno E, Mahfoud S, Moquet Torcy G, Gostan T, et al. AP-1 Signaling by Fra-1 Directly Regulates HMGA1 Oncogene Transcription in Triple-Negative Breast Cancers. Mol Cancer Res. 2019;17:1999-2014 pubmed 出版商
Baluapuri A, Hofstetter J, Dudvarski Stankovic N, Endres T, Bhandare P, Vos S, et al. MYC Recruits SPT5 to RNA Polymerase II to Promote Processive Transcription Elongation. Mol Cell. 2019;74:674-687.e11 pubmed 出版商
Zhang H, Pandey S, Travers M, Sun H, Morton G, Madzo J, et al. Targeting CDK9 Reactivates Epigenetically Silenced Genes in Cancer. Cell. 2018;175:1244-1258.e26 pubmed 出版商
Wong M, Belew M, Kwieraga A, Nhan J, Michael W. Programmed DNA Breaks Activate the Germline Genome in Caenorhabditis elegans. Dev Cell. 2018;46:302-315.e5 pubmed 出版商
Róna G, Roberti D, Yin Y, Pagan J, Homer H, Sassani E, et al. PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading. elife. 2018;7: pubmed 出版商
Fitzwalter B, Towers C, Sullivan K, Andrysik Z, Hoh M, Ludwig M, et al. Autophagy Inhibition Mediates Apoptosis Sensitization in Cancer Therapy by Relieving FOXO3a Turnover. Dev Cell. 2018;44:555-565.e3 pubmed 出版商
Yurko N, Liu X, Yamazaki T, Hoque M, Tian B, Manley J. MPK1/SLT2 Links Multiple Stress Responses with Gene Expression in Budding Yeast by Phosphorylating Tyr1 of the RNAP II CTD. Mol Cell. 2017;68:913-925.e3 pubmed 出版商
Butler A, Owens D, Wang L, King M. A novel role for sox7 in Xenopus early primordial germ cell development: mining the PGC transcriptome. Development. 2018;145: pubmed 出版商
Li Y, Wang C, Cai W, Sengupta S, Zavortink M, Deng H, et al. H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation. Development. 2017;144:3232-3240 pubmed 出版商
Yamashita T, Aizawa H, Teramoto S, Akamatsu M, Kwak S. Calpain-dependent disruption of nucleo-cytoplasmic transport in ALS motor neurons. Sci Rep. 2017;7:39994 pubmed 出版商
Oqani R, Lin T, Lee J, Choi K, Shin H, Jin D. P-TEFb Kinase Activity Is Essential for Global Transcription, Resumption of Meiosis and Embryonic Genome Activation in Pig. PLoS ONE. 2016;11:e0152254 pubmed 出版商
产品信息
抗原 :
RNA聚合酶II
Apps. Abbrev. :
西方墨点法, 免疫细胞化学, 免疫沉淀
目录 :
920204
克隆 :
H5
产品 :
纯化抗RNA聚合酶II RPB1
抗体亚型 :
Mouse IgM, κ
其他名称 :
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit
规格 :
100微克
价格(美元) :
253美元
反应物种 :
人类, 大鼠
克隆性 :
单克隆
宿主 :
小鼠
共轭标签/标签/标记 :
PURE
免疫原 :
The antibody H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line.
应用注释 :
Additional reported applications (for the relevant formats) include: immunoprecipitation 2,3 . The clone H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites: ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. Clone H5 recognizes the phosphoserine 2 (ser-2) of Pol II. Clone H5 is useful for immunofluorescence and Western blotting. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). This product weakly reacts with Mouse.
更多信息或购买 :
BioLegend 产品网页
公司信息
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
1-858-455-9588
公司总部: 美国
Biolegend公司为生物医学研究提供顶级的抗体及试剂产品，旨在加速生命科学领域的研发工作。