Babco, Signet, Sternberger Monoclonals, Senetek, Covance

抗体

纯化抗人类cdc2磷酸化(Thr14)

947402

100微升

329美元

单克隆

小鼠

未共轭

A20004B

免疫印迹, 免疫细胞化学

西方墨点法, 免疫细胞化学, 流式细胞仪细胞内染色

947402

A20004B

纯化抗人类cdc2磷酸化(Thr14)

Mouse IgG2b, κ

细胞周期蛋白依赖性激酶 1, CDC2, CDC28A, P34CDC2, P34蛋白激酶

100微升

329美元

人类

单克隆

小鼠

PURE

Synthetic peptide corresponding to human cdc2 phosphorylated at threonine 14

This clone is predicted to react with mouse cdc2 when phosphorylated at threonine 14 due to complete sequence homology between immunizing peptide and the cdc2 mouse orthologue. This clone was tested for ICC using lambda protein phosphatase analysis and HeLa cells fixed with methanol or 4% PFA followed by permeabilization with either methanol or Triton X-100. Only PFA fixation followed by methanol permeabilization produced phospho-specific staining of cdc2.