产品简要
公司名称 :
Biosensis
产品类型 :
抗体
产品名称 :
兔抗体乙NGF:IgG
目录 :
R-093-500
规格 :
500微克
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化
更多信息或购买 :
Biosensis 产品网页
文章摘录数: 2
已发表的文献中的应用方法／种属／稀释度参考文献
Lee H, Kim S, Shim N, Bae S, Jung I, Kwak J, et al. Expression of nerve growth factor is upregulated in the rat thymic epithelial cells during thymus regeneration following acute thymic involution. Regul Pept. 2007;141:86-95 pubmed
Laurina Z, Pilmane M, Care R. Growth factors/cytokines/defensins and apoptosis in periodontal pathologies. Stomatologija. 2009;11:48-54 pubmed
产品信息
目录代码 :
R-093-500
产品名称 :
兔抗体乙NGF:IgG
宿主物种 :
兔
克隆性 :
多克隆
产品类型 :
纯化
规格 :
500微克
Pubmed号列表 :
19773628, 17316840
产品描述 :
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
交叉活性 :
This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
别名 :
β神经生长因子
免疫原 :
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
特异性 :
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
包装 :
Small box at room temperature
应用摘要 :
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/ml is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/ml (ED50). This antibody was tested on cultured sensory neurons supported by 100ng/ml of purified mouse beta NGF. Be advised that 2ug/ml will neutralize 100ng/ml of mouse NGF. The higher 10ug/ml is only recommended if the concentration of NGF being used is higher than 100ng/ml such as the 200 or 500ng/ml that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
储存 :
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
产品引用 :
1. Laurina Z. et al (2009) Growth factors/cytokines/defensins and apoptosis in periodontal pathologies. Stomatologija. 2009;11(2):48-54. . 2. Lee H.W. et al (2007) Expression of nerve growth factor is upregulated in the rat thymic epithelial cells during thymus regeneration following acute thymic involution. Regul Pept. 2007 Jun 7;141(1-3):86-95 .
产品编号 :
160
更多信息或购买 :
Biosensis 产品网页
公司信息
Biosensis
39 Winwood Street, Thebarton, South Australia, 5031
info@biosensis.com
www.biosensis.com
61 8 83527711
公司总部: 澳大利亚
Biosensis specializes in antibodies, proteins, ELISA kits and Staining kits for Neuroscience, with particular emphasis on neurotrophins and neurotrophin receptors for which Biosensis has been the worldwide leader and OEM supplier for nearly 30 years. In addition to neurotrophins, our neuroscience portfolio includes products for research in neurodegenerative diseases, neurodevelopment, and neuro-metabolism. Areas of focus include Alzheimer's, Parkinson's and ALS diseases, as well as autophagy and metabolic and stress disorders including metabolic syndrome research, obesity, and neuroimmunology and inflammation.