抗体

Rabbit polyclonal antibody to NeuN/Fox3 (1-99): Whole serum

R-3770-100

100微升

297美元

多克隆

兔

nonconjugated

免疫印迹, 免疫细胞化学

Rabbit polyclonal antibody to NeuN/Fox3 (1-99): Whole serum

兔

多克隆

Whole Serum

100微升

297美元

24155322, 23560076, 22901764, 22540262

Fox3 is one of a family of mammalian homologues of Fox-1. The Fox proteins are about 46kDa in size, and each includes a central highly conserved RRM type RNA recognition motif. Much interest has focused on Fox3 as a result of the recent finding that this protein corresponds to NeuN, a neuronal nuclear antigen. NeuN/Fox-3 has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei and cytoplasm. Our antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.

Rat. Predicted to react with other mammals due to sequence homology.

Feminizing locus on X; Fox-1; Fox3; NeuN;

Antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli.

The specificity of this antibody has been confirmed by WB and IC. Two alternate transcripts can be seen at 46 kDa and 48 kDa in WB. This antibody has been used successfully for immunohistochemistry on paraformaldehyde fixed rat brain cortex (adult) tissue. It does not bind to the nuclei of perikarya of non-neuronal cells making it a good marker to identify neurons.

Small box at room temperature

Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500 - 1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

. Saha M, et al (2013) Spinal Mitogen-Activated Protein Kinase Phosphatase-3 (MKP-3) is Necessary for the Normal Resolution of Mechanical Allodynia in a Mouse Model of Acute Postoperative Pain. J. Neurosci. 33(43):17182-7 . Liu X et al (2013) Suppressive Effect of Phenol Red on the Epileptiform Burst Activity via Activation of Estrogen Receptors in Primary Hippocampal Culture. PLoS ONE 8(4):e60189 . Landry R.P. et al (2012) Spinal Cannabinoid Receptor Type 2 Agonist Reduces Mechanical Allodynia and Induces Mitogen-Activated Protein Kinase Phosphatases in a Rat Model of Neuropathic Pain. J Pain. 2012 Aug 14 . Ndong C. et al (2012) Mitogen activated protein kinase phosphatase-1 prevents the development of tactile sensitivity in a rodent model of neuropathic pain. Mol Pain. 2012 Apr 27;8(1):34. .

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