抗体

羊抗体alpha突触核蛋白(116-131):亲和纯化

S-075-50

50微克

297美元

多克隆

羊

nonconjugated

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫组化-石蜡切片

羊抗体alpha突触核蛋白(116-131):亲和纯化

羊

多克隆

纯化

50微克

297美元

20106867, 20533994, 18385100

Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.

This antibody is known to react with alpha synuclein of human, mouse, rat and other rodents.

Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP

A synthetic peptide (CMPVDPDNEAYEMPSEE) of human alpha synuclein protein (aa: 116-131) conjugated to diphtheria toxoid has been used as the immunogen.

This antiserum specifically detects alpha synuclein.

Small box at room temperature

IHC, WB, ELISA. Recommended to be used at a concentration of 1µg/ml for immunohistochemistry (Paraffin sections); 0.5 µg/ml for WB and immunoblot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

1. Kuo Y.M. et al. (2010) Extensive enteric nervous system abnormalities in mice transgenic for artificial chromosomes containing Parkinson disease-associated alpha-synuclein gene mutations precede central nervous system changes. Hum Mol Genet. May 1;19(9):1633-50 . 2. Pelkonen A. et al. (2010) Stimulated dopamine overflow and alpha-synuclein expression in the nucleus accumbens core distinguish rats bred for differential ethanol preference. J Neurochem. 2010 Aug;114(4):1168-76. . 3. Alves et al. (2008) Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease Hum Mol Genet. 2008 Jul 15;17(14):2071-83. .

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