ELISA/assay

人类白细胞介素8(IL-8)酶联免疫吸附试剂盒

BE010003

96 well

473.00美元

人类白细胞介素8(IL-8)酶联免疫吸附试剂盒

酶联免疫吸附试剂盒

96 well

473.00美元

P10145

Interleukin-8 (IL-8), also known as neutrophil attractant/activating protein (NAP-1), monocyte-derived neutrophil-activating peptide (MONAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T lymphocyte chemotactic factor (TCF) and leukocyte adhesion inhibitor (LAI), is a member of the chemokine superfamily which selectively chemoattract and activate specific leukocyte subpopulations (1,2). All of these cytokines have four conserved cysteines and two distinguishable subfamilies. These two subfamilies are dependent on the position of the first two cysteines, which are either separated by on amino acid (C-X-C proteins) or are adjacent (CC-protein) to each other. The members of the two subfamilies differ in their target cell selectivity as well as the chromosomal location of their genes (chromosome 4 for the C-X-C proteins and chromosome 17 for the C-C proteins). IL-8 belongs to the C-X-C subfamily along with platelet factor 4 (PF4), platelet basic protein (PBP), connective-tissue-activating peptide III (CTAPIII), b-thromboglobulin, neutrophil-activating peptide-2 (NAP-2), ENA-78 (3), three closely related MGSA/CRO gene products (GRO-a, GRO-b, GRO-g), and g-interferon-inducible protein (g-IP-10)(4). The members of the C-C chemokines are mainly chemotactic for monocytes whereas the C-X-C chemokines except for IP10 and PF4, chemoattract and activate neutrophils. Various non-infectious human diseases are known to be associated with neutrophilia and/or neutrophil infiltration into organs. Examples of some of these human diseases include rheumatoid arthritis, gouty arthritis, psoriasis, glomerulonephritis, adult respiratory distress syndrome, immune vasculitis, inflammatory bowel disease, ischemia-reperfusion syndrome (including myocardial infarction and multiple organ failure), chorioretinitis, cystic fibrosis, septic shock, acute meningococcal infections, alcoholic hepatitis and Mediterranean fever (8). In addition to the effect on neutrophils, IL-8 has been reported to be a less potent chemoattractant for T lymphocytes (5). IL-8 is produced by many cells in response to inflammatory stimuli such as IL-1b or TNF-a and to various types of mitogen, lectins, crystals, viruses, and phorbol esters (PMA). Many cell types that produce IL-8 in response to these stimuli can include: monocytes/ macrophages, T lymphocytes, neutrophils, fibroblasts, keratinocytes, hepacytes, chondrocytes, endothelial cells, glioblatoma cells, and mesothelial cells (6). In addition to recruiting and activating neutrophils, IL-8 may elicit many other activities that contribute to diseases. However, IL-8 is not known to trigger systemic inflammatory reactions such as fever, acute phase protein induction. This IL-8 ELISA is a 2.5 hour solid-phase immunoassay readily applicable to measure IL-8 levels in cell culture supernatant, serum, plasma, and other biological fluids ranging from 0 to 800pg/mL. It has shown no cross-reactivity with human monocyte chemotactic activating factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted). This IL-8 ELISA is expected to be useful for further investigations into the role of IL-8 in inflammatory reaction and immune response.

存放在4°C。 不冻结