抗钙粘蛋白N CDH2 CD325单克隆抗体
武汉博士德生物工程有限公司
目录: MA1067
小鼠 单克隆 (GC-4)
反应物种: 人类, 小鼠, 大鼠, 鸡
应用: 免疫印迹, 免疫组化

Figure 1. Western blot analysis of Cadherin-2/N-Cadherin using anti- Cadherin-2/N-Cadherin antibody (MA1067). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human A431 whole cell lysates,. Lane 3: human MCF-7 whole cell lysates, . Lane 4: human PANC-1 whole cell lysates,. Lane 5: human A549 whole cell lysates,. Lane 6: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Cadherin-2/N-Cadherin antigen affinity purified monoclonal antibody (Catalog # MA1067) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cadherin-2/N-Cadherin at approximately 130KD. The expected band size for Cadherin-2/N-Cadherin is at 100KD.
规格: 100μg/vial
价格: 315美元
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抗钙粘蛋白N CDH2 CD325抗体
武汉博士德生物工程有限公司
目录: PA1328
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Figure 1. Western blot analysis of N Cadherin using anti-N Cadherin antibody (PA1328). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-N Cadherin antigen affinity purified polyclonal antibody (Catalog # PA1328) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for N Cadherin at approximately 140KD. The expected band size for N Cadherin is at 100KD.

Anti-N Cadherin antibody, PA1328, IHC(P). IHC(P): Human Ovary Cancer Tissue
规格: 100μg/vial
价格: 315美元
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抗钙粘蛋白N CDH2 CD325兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01577-1
domestic rabbit 单克隆 (FI-3)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Western blot analysis of N-Cadherin expression in HeLa cell lysate (M01577-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 monoclonal antibody (Catalog # M01577-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2
规格: 100微升
价格: 315美元
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抗钙粘蛋白N CDH2 CD325抗体
武汉博士德生物工程有限公司
目录: A01577-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
规格: 100微升
价格: 697美元
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Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01577-2
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹
规格: 100µg/vial
价格: 315美元
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Anti-N Cadherin/CDH2 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01577-3
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
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抗钙粘蛋白N CDH2 CD325兔单克隆抗体, Clone#RM259
武汉博士德生物工程有限公司
目录: M01577-2
domestic rabbit 单克隆 (RM259)
反应物种: 人类
应用: 免疫印迹
规格: 100微升
价格: 399美元
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