抗CEACAM5/Cd66e单克隆抗体
武汉博士德生物工程有限公司
目录: MA1023
小鼠 单克隆 (C6G9)
反应物种: 人类
应用: 免疫印迹, 免疫组化

Figure 1. Western blot analysis of CEACAM5/Cd66e using anti- CEACAM5/Cd66e antibody (MA1023). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Recombinant Human CEACAM5/Cd66e Protein 10ng. Lane 2; Recombinant Human CEACAM5/Cd66e Protein 5ng. Lane 3: Recombinant Human CEACAM5/Cd66e Protein 2.5ng. Lane 4: Recombinant Human CEACAM5/Cd66e Protein 1.25ng . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- CEACAM5/Cd66e antigen affinity purified monoclonal antibody (Catalog # MA1023) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

Figure 2. IHC analysis of CEACAM5/Cd66e using anti-CEACAM5/Cd66e antibody (MA1023). CEACAM5/Cd66e was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-CEACAM5/Cd66e Antibody (MA1023) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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抗CEACAM5抗体
武汉博士德生物工程有限公司
目录: RP1018
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化

Figure 1. Western blot analysis of CEA using anti- CEA antibody (RP1018). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, . Lane 2: human Caco-2 whole cell lysates, . Lane 3: mouse small intestine tissue lysates, . Lane 4: mouse stomach tissue lysates,. Lane 5: mouse lung tissue lysates,. Lane 6: mouse liver tissue lysates,. Lane 7: mouse NIH3T3 whole cell lysates,. Lane 8: mouse HEPA1-6 whole cell lysates,. Lane 9: mouse SP20 whole cell lysates,. Lane 10: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CEA antigen affinity purified polyclonal antibody (Catalog # RP1018) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.
规格: 100μg/vial
价格: 315美元
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Anti-CEACAM5 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00356
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
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Anti-CEA Antibody (Center)
武汉博士德生物工程有限公司
目录: A00356-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
规格: 80微升
价格: 166美元
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抗CEA(CD66e)CEACAM5兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00356-1
domestic rabbit 单克隆 (AAOA-3)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 315美元
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抗CEA(CD66e)兔单克隆抗体, Clone#RM326
武汉博士德生物工程有限公司
目录: M00356-3
domestic rabbit 单克隆 (RM326)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
规格: 100微升
价格: 399美元
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