domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
Figure 1. Western blot analysis of -CIAS1/NALP3 using anti- -CIAS1/NALP3 antibody (PA1665). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEP-2 Cell Lysate. Lane 2: A549 Cell Lysate . Lane 3: U87 Cell Lysate. Lane 4: CEM Cell Lysate . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- -CIAS1/NALP3 antigen affinity purified polyclonal antibody (Catalog # PA1665) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for -CIAS1/NALP3 at approximately 118KD. The expected band size for -CIAS1/NALP3 is at 118KD.
Figure 2. IHC analysis of CIAS1/NALP3 using anti- CIAS1/NALP3 antibody (PA1665). CIAS1/NALP3 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CIAS1/NALP3 Antibody (PA1665) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
Anti-CIAS1/NALP3 Picoband antibody, PB9114-1.jpg. All lanes: Anti CIAS1 (PB9114) at 0.5ug/ml. WB: Recombinant Human CIAS1 Protein 0.5ng. Predicted bind size: 37KD. Observed bind size: 37KD
Anti-CIAS1/NALP3 Picoband antibody, PB9114-2.jpg. All lanes: Anti CIAS1 (PB9114) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: MCF-7 Whole Cell Lysate at 40ug. Lane 3: JURKAT Whole Cell Lysate at 40ug. Lane 4: HEPG2 Whole Cell Lysate at 40ug. Predicted bind size: 118KD. Observed bind size: 118KD
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 单克隆 (FBD-14)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
Western blot analysis of NALP3 using anti-NALP3 antibody (M00034) on SH-SY5Y cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NALP3 antigen affinity purified polyclonal antibody (Catalog # M00034) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NALP3.
IHC analysis of NALP3 using anti-NALP3 antibody (M00034) on human lung carcinoma. NALP3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NALP3 Antibody (M00034) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IF analysis of immunocytochemical section of Raw264.7 cells using anti- NALP3 antibody (M00034) . NALP3 was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- NALP3 Antibody (M00034) overnight at 4°C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
规格: 100微升
价格: 315美元
至产商
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪
微博分享
关注我们的微博
- 来邦网
- 英文来邦