Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC02236
小鼠 单克隆 (OKT8)
反应物种: 人类, 小鼠
应用: 流式细胞仪
规格: 100微克, 500微克
价格: 114美元
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Anti-CD8 alpha/Cd8a Antibody Picoband™
武汉博士德生物工程有限公司
目录: A02236-1
domestic rabbit 多克隆
反应物种: 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪

Figure 1. Western blot analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate,. Lane 2: rat thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8 alpha antigen affinity purified polyclonal antibody (Catalog # A02236-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8 alpha at approximately 36KD. The expected band size for CD8 alpha is at 26KD.

Figure 2. IHC analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1). CD8 alpha was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD8 alpha Antibody (A02236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1). CD8 alpha was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD8 alpha Antibody (A02236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
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Anti-human CD8 Cd8a Monoclonal Antibody FITC Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC02236-FITC
小鼠 单克隆 (OKT8)
反应物种: 人类, 小鼠
共轭标签: 异硫氰酸荧光素
应用: 流式细胞仪
规格: 100试验, 200试验
价格: 132美元
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抗小鼠CD8甲DyLight系列染料®488共轭标签抗体
武汉博士德生物工程有限公司
目录: A02236-Dyl488
domestic rabbit 多克隆
反应物种: 小鼠
共轭标签: DyLight488
应用: 流式细胞仪

Boster Kit Box
规格: 50 ug/vial
价格: 415美元
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抗小鼠CD8甲DyLight系列染料®550共轭标签抗体
武汉博士德生物工程有限公司
目录: A02236-Dyl550
domestic rabbit 多克隆
反应物种: 小鼠
共轭标签: DyLight系列染料550
应用: 流式细胞仪

Boster Kit Box
规格: 50 ug/vial
价格: 415美元
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Anti-CD8 alpha/Cd8a Antibody Picoband™
武汉博士德生物工程有限公司
目录: A02236-3
domestic rabbit 多克隆 (polyclonal)
反应物种: 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
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Anti-human CD8 Cd8a Monoclonal Antibody APC Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC02236-APC
小鼠 单克隆 (OKT8)
反应物种: 小鼠
共轭标签: APC
应用: 流式细胞仪
规格: 100试验, 200试验
价格: 312美元
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Anti-human CD8 Cd8a Monoclonal Antibody Biotin Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC02236-Biotin
小鼠 单克隆 (OKT8)
反应物种: 小鼠
共轭标签: 生物素
应用: 流式细胞仪
规格: 100µg
价格: 226美元
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Anti-human CD8 Cd8a Monoclonal Antibody PE Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC02236-PE
小鼠 单克隆 (OKT8)
反应物种: 小鼠
共轭标签: PE
应用: 流式细胞仪
规格: 100试验, 200试验
价格: 269美元
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Anti-human CD8 Cd8a Monoclonal Antibody PerCP Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC02236-PerCP
小鼠 单克隆 (OKT8)
反应物种: 小鼠
共轭标签: PerCP
应用: 流式细胞仪
规格: 100试验, 200试验
价格: 338美元
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Anti-human CD8 Cd8a Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC02236-PerCP-Cy5.5
小鼠 单克隆 (OKT8)
反应物种: 小鼠
共轭标签: PerCP/Cy5.5
应用: 流式细胞仪
规格: 100试验, 200试验
价格: 360美元
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Anti-Ms CD8a Purified Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M02236
大鼠 单克隆 (53-6.7)
反应物种: 小鼠
应用: 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
规格: 0.1毫克(C100)
价格: 280美元
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