domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
共轭标签: 生物素
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
反应物种: 人类, 小鼠, 大鼠
共轭标签: 生物素
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
Western blot analysis of Collagen I expression in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (lane 1), PPARg-transduced cell lysates (lane 2). Load: 100 ug per lane. Collagen I at 138.9 kDa was detected using biotin conjugated rabbit anti-Collagen Type I Antigen Affinity purified polyclonal antibody (Catalog # B00329) at 0.2–2 ug/10 ml. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Collagen Type I was detected in paraffin-embedded sections of human skin at pH6 using biotin conjugated rabbit anti-Collagen Type I Antigen Affinity purified polyclonal antibody (Catalog # B00329) at 10 ug/ml. The immunohistochemical sections were developed using SABC method (Catalog # SA1022). Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).
Collagen Type I was detected in paraffin-embedded sections of human skin at pH6 using biotin conjugated rabbit anti-Collagen Type I Antigen Affinity purified polyclonal antibody (Catalog # B00329) at 10 ug/ml. The immunohistochemical sections were developed using SABC method (Catalog # SA1022). Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).
规格: 100微克
价格: 496美元
至产商
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化
反应物种: 人类
应用: 免疫印迹, 免疫组化
Western blot analysis of Collagen I expression in MCF-7 whole cell lysates (lane 1). Collagen I at 180KD;55KD was detected using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody (Catalog # PB9938) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Collagen I was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody (Catalog # PB9938) at 1 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片
Figure 1. IHC analysis of Collagen I/COL1A1 using anti- Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in paraffin-embedded section of Rat Lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of Collagen I/COL1A1 using anti- Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in paraffin-embedded section of Human Intestinal Cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Collagen I/COL1A1 using anti- Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in immunocytochemical section of NIH3T3 Cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
小鼠 单克隆 (monoclonal)
反应物种: 人类
应用: 免疫细胞化学, 免疫组化-石蜡切片
反应物种: 人类
应用: 免疫细胞化学, 免疫组化-石蜡切片
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