抗胶原, 类型III Col3a1抗体(单克隆, FH-7A)
武汉博士德生物工程有限公司
目录: MA1029
小鼠 单克隆 (FH-7A)
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化

Anti-Collagen, Type III antibody (monoclonal), MA1029, Western blotting. Lane 1: HT1080 Cell Lysate. Lane 2: COLO320 Cell Lysate.

Anti-Collagen, Type III antibody (monoclonal), MA1029, IHC(F). IHC(F): Mouse Kidney Tissue
规格: 100μg/vial
价格: 315美元
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Anti-Collagen IV/COL4A1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9099
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Figure 1. Western blot analysis of Collagen IV using anti-Collagen IV antibody (PB9099). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen IV antigen affinity purified polyclonal antibody (Catalog # PB9099) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen IV at approximately 250KD. The expected band size for Collagen IV is at 161KD.

Figure 2. IHC analysis of Collagen Ⅳ using anti-Collagen Ⅳ antibody (PB9099). Collagen Ⅳ was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Collagen Ⅳ Antibody (PB9099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IF analysis of COL4A1 using anti- COL4A1 antibody (PB9099) . COL4A1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- COL4A1 Antibody (PB9099) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
规格: 100μg/vial
价格: 315美元
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抗胶原III/COL3A1抗体
武汉博士德生物工程有限公司
目录: RP1033
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Anti-Collagen III Picoband antibody, RP1033-1.jpg. All lanes: Anti Collagen III (RP1033) at 0.5ug/ml. WB: Recombinant Human Collagen III Protein 0.5ng. Predicted bind size: 39KD. Observed bind size: 39KD

Anti-Collagen III Picoband antibody, RP1033-2.jpg. All lanes: Anti Collagen III(RP1033) at 0.5ug/ml. Lane 1: MCF-4 Whole Cell Lysate at 40ug. Lane 2: COLO320 Whole Cell Lysate at 40ug. Predicted bind size: 139KD. Observed bind size: 139KD
规格: 100μg/vial
价格: 315美元
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Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9)
武汉博士德生物工程有限公司
目录: M00788
小鼠 单克隆 (9H9)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化-石蜡切片

Boster Kit Box
规格: 100µg/vial
价格: 315美元
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抗胶原III COL3A1抗体
武汉博士德生物工程有限公司
目录: A00788-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫组化, 免疫细胞化学
规格: 100µl
价格: 299美元
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抗胶原III兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00788-1
domestic rabbit 单克隆 (26C74)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪
规格: 100 µl/vial
价格: 315美元
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