Anti-EGFR Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9863
domestic rabbit 多克隆
反应物种: 小鼠
应用: 免疫印迹

Western blot analysis of EGFR expression in mouse liver extract (lane 1). EGFR at 230KD was detected using rabbit anti- EGFR Antigen Affinity purified polyclonal antibody (Catalog # PB9863) at0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
规格: 100μg/vial
价格: 315美元
至产商
抗磷酸化表皮生长因子受体(Y1092)兔单克隆抗体
武汉博士德生物工程有限公司
目录: P00023
domestic rabbit 单克隆 (BFO-5)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Phospho-EGFR (Y1092) Antibody (P00023). EGFR was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EGFR Antibody (P00023)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of EGFR phosphorylation expression in Huvec cell lysate treated with EGF (P00023). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
规格: 100微升
价格: 315美元
至产商
Anti-EGFR (ErbB 1) Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M00023-2
domestic rabbit 单克隆 (AEF-5)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪

Figure 2. IHC analysis of EGFR using anti-EGFR antibody (M00023-2)on paraffin-embedded human stomach cancer. EGFR was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EGFR Antibody (M00023-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of EGFR using anti-EGFR antibody (M00023-2) in HeLa cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (Catalog # M00023-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for EGFR.
规格: 100微升
价格: 315美元
至产商
Anti-EGFR Picoband™ Antibody
武汉博士德生物工程有限公司
目录: A00023-2
domestic rabbit 多克隆 (polyclonal)
反应物种: 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
至产商
抗磷酸化表皮生长因子受体(S1026)抗体
武汉博士德生物工程有限公司
目录: A00023S1026
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100微升
价格: 380美元
至产商
抗表皮生长因子受体(S1064)抗体
武汉博士德生物工程有限公司
目录: A00023S1064
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 512美元
至产商
抗磷酸化表皮生长因子受体(S1070)抗体
武汉博士德生物工程有限公司
目录: A00023S1070
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
规格: 100微升
价格: 380美元
至产商
抗磷酸化表皮生长因子受体(S1071)抗体
武汉博士德生物工程有限公司
目录: A00023S1071
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100微升
价格: 380美元
至产商
抗表皮生长因子受体(磷酸化Y992)抗体
武汉博士德生物工程有限公司
目录: A00023Y992-1
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 547美元
至产商
抗表皮生长因子受体(磷酸化Tyr998)抗体
武汉博士德生物工程有限公司
目录: A00023Y998-1
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 380美元
至产商