Anti-PARP/PARP1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9309
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Figure 1. Western blot analysis of PARP using anti-PARP antibody (PB9309). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human HepG2 whole cell lysates,. Lane 3: human COLO-320 whole cell lysates, . Lane 4: human Jurkat whole cell lysates,. Lane 5: rat PC-12 whole cell lysates,. Lane 6: mouse NIH3T3 whole cell lysates,. Lane 7: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP antigen affinity purified polyclonal antibody (Catalog # PB9309) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP at approximately 120KD. The expected band size for PARP is at 113KD.

Figure 2. IHC analysis of PARP using anti-PARP antibody (PB9309). PARP was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PARP Antibody (PB9309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of PARP using anti-PARP antibody (PB9309). PARP was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PARP Antibody (PB9309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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抗切断的PARP多聚(ADP-核糖)聚合酶兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00122-1
domestic rabbit 单克隆 (HI-16)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Western blot analysis of Cleaved PARP expression in (1) RAW2647 cell lysate;(2) NIH/3T3 cell lysate (M00122-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 monoclonal antibody (Catalog # M00122-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1

Immunohistochemical analysis of paraffin-embedded human kidney, using Cleaved PARP Antibody(M00122-1). PARP1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PARP1 Antibody (M00122-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100微升
价格: 315美元
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Anti-PARP Picoband™ Antibody (monoclonal, 2I2H4)
武汉博士德生物工程有限公司
目录: M00122-4
小鼠 单克隆 (2I2H4)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
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Anti-PARP Picoband™ Antibody (monoclonal, 10E11)
武汉博士德生物工程有限公司
目录: M00122-5
小鼠 单克隆 (110)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
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Anti-PARP Picoband™ Antibody (monoclonal, 10G9)
武汉博士德生物工程有限公司
目录: M00122-6
小鼠 单克隆 (10G9)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
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