Anti-Cystatin C/CST3 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00961-2
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化

Figure 1. Western blot analysis of Cystatin C using anti-Cystatin C antibody (A00961-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates,. Lane 2: human placenta tissue lysates, . Lane 3: human HepG2 whole cell lysates,. Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin C antigen affinity purified polyclonal antibody (Catalog # A00961-2) at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin C at approximately 16KD. The expected band size for Cystatin C is at 16KD.

Figure 2. IHC analysis of Cystatin C using anti-Cystatin C antibody (A00961-2). Cystatin C was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cystatin C Antibody (A00961-2) overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
至产商
Anti-Cystatin C/CST3 Antibody Picoband™ (monoclonal, 4H8)
武汉博士德生物工程有限公司
目录: M00961-1
小鼠 单克隆 (4H8)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Boster Kit Box
规格: 100µg/vial
价格: 315美元
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Anti-Cystatin C/CST3 Picoband™ Biotinylated Antibody (monoclonal, 8D1)
武汉博士德生物工程有限公司
目录: M00961-Biotin
小鼠 单克隆 (8D1)
反应物种: 人类
应用: 酶联免疫吸附测定

Boster Kit Box
规格: 50µg/vial
价格: 315美元
至产商
Anti-Cystatin C/CST3 Antibody Picoband™ (monoclonal, 8H7)
武汉博士德生物工程有限公司
目录: M00961-3
小鼠 单克隆 (8H7)
反应物种: 人类
应用: 酶联免疫吸附测定

Boster Kit Box
规格: 100µg/vial
价格: 315美元
至产商
抗胱蛋白C CST3兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00961
domestic rabbit 单克隆 (IEE-3)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Western blot analysis of Cystatin C expression in HeLa cell lysate (M00961). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CST3 monoclonal antibody (Catalog # M00961) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CST3
规格: 100微升
价格: 315美元
至产商
抗胱蛋白C CST3抗体
武汉博士德生物工程有限公司
目录: A00961-4
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 697美元
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