抗MEK1/MAP2K1抗体
武汉博士德生物工程有限公司
目录: PA1376
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Anti-MEK1 antibody, PA1376, Western blotting. Lane 1: Rat Skeletal Muscle Tissue Lysate. Lane 2: Rat Kidney Tissue Lysate. Lane 3: CEM Cell Lysate. Lane 4: COLO20 Cell Lysate

Anti-MEK1 antibody, PA1376, IHC(P). IHC(P): Human Lung Cancer Tissue

Anti-MEK1 antibody, PA1376, IHC(P). IHC(P): Human Mammary Cancer Tissue
规格: 100μg/vial
价格: 315美元
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抗MEK1/2 MAP2K1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00292-1
domestic rabbit 单克隆 (BOA-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

Immunohistochemical analysis of paraffin-embedded human kidney, using MEK1/2 Antibody(M00292-1). MAP2K1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K1 Antibody (M00292-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of MEK1/2 expression in (1) 293T cell lysate; (2) A549 cell lysate (M00292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # M00292-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
规格: 100微升
价格: 315美元
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抗MEK1 MAP2K1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00292-2
domestic rabbit 单克隆 (IF-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪

Western blot analysis of MEK1 expression in (1) A431 cell lysate;(2) HeLa cell lysate (M00292-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # M00292-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
规格: 100微升
价格: 315美元
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抗磷酸化MEK1(S298)MAP2K1兔单克隆抗体
武汉博士德生物工程有限公司
目录: P00292-3
domestic rabbit 单克隆 (HBO-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Western blot analysis of Phospho-MEK1 (S298) expression in (1) HeLa cell treated with LP lysate; (2) HeLa cell lysate (P00292-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # P00292-3) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
规格: 100微升
价格: 315美元
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