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武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 TUBB 抗体
Anti-Beta Tubulin/TUBB Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01857-1
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
,
免疫组化-冰冻切片
Western blot analysis of Beta Tubulin expression in rat brian extract (lane 1), mouse brain extract (lane 2) and HELA whole cell lysates (lane 3). Beta Tubulin at 55KD was detected using rabbit anti- Beta Tubulin Antigen Affinity purified polyclonal antibody (Catalog # A01857-1) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Beta Tubulin was detected in paraffin-embedded sections of human glioma tissues using rabbit anti- Beta Tubulin Antigen Affinity purified polyclonal antibody (Catalog # A01857-1) at 1 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Beta Tubulin was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- Beta Tubulin Antigen Affinity purified polyclonal antibody (Catalog # A01857-1) at 1 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
规格: 100μg/vial
价格: 315美元
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抗β微管蛋白TUBB兔单克隆抗体
武汉博士德生物工程有限公司
目录: M05613
domestic rabbit 单克隆 (BI-20)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
流式细胞仪
Immunofluorescent analysis of NIH/3T3 cells, using beta Tubulin Antibody
Immunohistochemical analysis of paraffin-embedded (1) Human glioma; (2) Rat brain; (3) Mouse kidney; (4) Human uterus cancer, using beta Tubulin Antibody(M05613). TUBB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB Antibody (M05613)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of beta Tubulin expression in (1) MCF-7 cell lysate; (2) COS-1 cell lysate; (3) Jurkat cell lysate; (4) HeLa cell lysate (M05613). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB monoclonal antibody (Catalog # M05613) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB
规格: 100微升
价格: 315美元
至产商
Anti-Beta Tubulin Antibody Picoband™(monoclonal, 5E4)
武汉博士德生物工程有限公司
目录: M01857-3
小鼠 单克隆 (50000)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
规格: 100µg/vial
价格: 315美元
至产商
Anti-Beta Tubulin TUBB Antibody Picoband™ (monoclonal, 2E11)
武汉博士德生物工程有限公司
目录: M05613-4
小鼠 单克隆 (2E+11)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
规格: 100µg/vial
价格: 315美元
至产商
抗β微管蛋白小鼠单克隆抗体
武汉博士德生物工程有限公司
目录: M05613-5
小鼠 单克隆 (17T13)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
规格: 100 µl/vial
价格: 315美元
至产商
抗β微管蛋白兔单克隆抗体
武汉博士德生物工程有限公司
目录: M05613-6
domestic rabbit 单克隆 (17T15)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
流式细胞仪
规格: 100 µl/vial
价格: 315美元
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