抗β微管蛋白TUBB兔单克隆抗体
武汉博士德生物工程有限公司
目录: M05613
domestic rabbit 单克隆 (BI-20)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Immunofluorescent analysis of NIH/3T3 cells, using beta Tubulin Antibody

Immunohistochemical analysis of paraffin-embedded (1) Human glioma; (2) Rat brain; (3) Mouse kidney; (4) Human uterus cancer, using beta Tubulin Antibody(M05613). TUBB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB Antibody (M05613)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of beta Tubulin expression in (1) MCF-7 cell lysate; (2) COS-1 cell lysate; (3) Jurkat cell lysate; (4) HeLa cell lysate (M05613). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB monoclonal antibody (Catalog # M05613) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB
规格: 100微升
价格: 315美元
至产商
Anti-Beta Tubulin Antibody Picoband™(monoclonal, 5E4)
武汉博士德生物工程有限公司
目录: M01857-3
小鼠 单克隆 (50000)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
规格: 100µg/vial
价格: 315美元
至产商
Anti-Beta Tubulin TUBB Antibody Picoband™ (monoclonal, 2E11)
武汉博士德生物工程有限公司
目录: M05613-4
小鼠 单克隆 (2E+11)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
规格: 100µg/vial
价格: 315美元
至产商
抗β微管蛋白小鼠单克隆抗体
武汉博士德生物工程有限公司
目录: M05613-5
小鼠 单克隆 (17T13)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100 µl/vial
价格: 315美元
至产商
抗β微管蛋白兔单克隆抗体
武汉博士德生物工程有限公司
目录: M05613-6
domestic rabbit 单克隆 (17T15)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
规格: 100 µl/vial
价格: 315美元
至产商