抗AKT抗体生物素共轭标签
武汉博士德生物工程有限公司
目录: B00024
兔 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
规格: 100微升
价格: 339美元
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抗AKT1/2抗体
武汉博士德生物工程有限公司
目录: PA1301
兔 多克隆
反应物种: 人类
应用: 免疫印迹
规格: 100 ug/vial
价格: 200美元
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Anti-Phospho-Akt (Ser473) Rabbit Monoclonal Antibody
武汉博士德生物工程有限公司
目录: P00024-5
兔 单克隆 (IFD-1)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Western blot analysis on 2 NIH/3T3 cell lysate treated with PDGF using Phospho-Akt(Ser473) Antibody (P00024-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody (Catalog # P00024-5) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1

Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-Akt(Ser473) Antibody(P00024-5). AKT1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AKT1 Antibody (P00024-5)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100 ug/vial
价格: 299美元
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抗AKT1单克隆抗体
武汉博士德生物工程有限公司
目录: M00024-1
兔 单克隆 (EBG-1)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

Immunofluorescent analysis of Hela cells, using AKT1 Antibody.

Figure 2. IHC analysis of AKT1 using anti-AKT1 antibody (M00024-1). AKT1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AKT1 Antibody (M00024-1) overnight at 4 C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of AKT1 using anti-AKT1 antibody (M00024-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified polyclonal antibody (Catalog # M00024-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for AKT1.
规格: 100微克
价格: 299美元
至产商