兔 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫组化, 免疫细胞化学
反应物种: 人类, 小鼠, 大鼠
应用: 免疫组化, 免疫细胞化学
Figure 2. IHC analysis of ESR2 using anti-ESR2 antibody (A00786). ESR2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESR2 Antibody (A00786) overnight at 4 C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1: Estrogen receptor beta, pAb (Prod. No. A00786) used in immunofluorescence.
规格: 50微克
价格: 563美元
至产商
兔 多克隆
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
Anti-Estrogen Receptor beta antibody, PA1126, Western blotting. WB: MCF-7 Cell Lysate
规格: 100 ug/vial
价格: 240美元
至产商
兔 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
Figure 1. Western blot analysis of ESR2 using anti-ESR2 antibody (A00786-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate,. Lane 2: rat ovary tissue lysate,. Lane 3: mouse testis tissue lysate,. Lane 4: mouse ovary tissue lysate,. Lane 5: human Hela whole Cell lysate,. Lane 6: human MCF-7 whole Cell lysate,. Lane 7: human MDA-MB-231 whole Cell lysate,. Lane 8: human MDA-MB-453 whole Cell lysate,. Lane 9: human SK-OV-3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 antigen affinity purified polyclonal antibody (Catalog # A00786-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2 at approximately 59KD. The expected band size for ESR2 is at 59KD.
规格: 100 ug/vial
价格: 240美元
至产商
兔 单克隆 (IDI-5)
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
Western blot analysis of Estrogen Receptor beta expression in MCF-7 Cell Lysate (M00786-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 monoclonal antibody (Catalog # M00786-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2
规格: 100 ug/vial
价格: 299美元
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