兔 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
Anti- Vimentin Picoband antibody, PB9359, Western blotting. All lanes: Anti Vimentin (PB9359) at 0.5ug/ml. Lane 1: HT1080 Whole Cell Lysate at 40ug. Lane 2: NIH Whole Cell Lysate at 40ug. Lane 3: JURKAT Whole Cell Lysate at 40ug. Lane 4: HUT Whole Cell Lysate at 40ug. Lane 5: MCF-7 Whole Cell Lysate at 40ug. Lane 6: HELA Whole Cell Lysate at 40ug. Lane 7: Human Placenta Tissue Lysate at 50ug. Lane 8: Rat Testis Tissue Lysate at 50ug. Lane 9: Mouse Testis Tissue Lysate at 50ug. Predicted bind size: 54KD. Observed bind size: 54KD
Anti- Vimentin Picoband antibody, PB9359, IHC(P). IHC(P): Mouse Cardiac Muscle Tissue
Anti- Vimentin Picoband antibody, PB9359, IHC(P). IHC(P): Rat Cardiac Muscle Tissue
规格: 100 ug/vial
价格: 280美元
至产商
兔 单克隆 (AGF-22)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody(M00235-1). VIM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VIM Antibody (M00235-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of Vimentin expression in HEK293 cell lysate (M00235-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VIM monoclonal antibody (Catalog # M00235-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VIM
规格: 100 ug/vial
价格: 299美元
至产商
兔 多克隆
反应物种: 人类, 小鼠, 大鼠, 牛
应用: 免疫印迹, 免疫组化
反应物种: 人类, 小鼠, 大鼠, 牛
应用: 免疫印迹, 免疫组化
Western blot analysis of whole cell lysates using rabbit pAb to vimentin, M00235-4, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa.
Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, M00235-4, dilution 1:5,000, in green, and mouse mAb to Actin, dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
规格: 100微升
价格: 299美元
至产商
鸡 多克隆
反应物种: 人类, 小鼠, 大鼠, 鸡, 牛
应用: 免疫印迹, 免疫组化
反应物种: 人类, 小鼠, 大鼠, 鸡, 牛
应用: 免疫印迹, 免疫组化
Western blot analysis of tissue and cell lysates using chicken pAb to Vimentin, M00235-5, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. M00235-5 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes, and ~70kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
Immunofluorescent analysis of HeLa cell culture stained with chicken pAb to vimentin, M00235-5, dilution 1:10,000 in green, and costained with Boster mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
规格: 100微升
价格: 299美元
至产商
兔 单克隆 (ADE-22)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫沉淀
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫沉淀
Western blot analysis of Phospho-Vimentin (Ser72) in HeLa cell lysates treated with Calyculin A (P00235). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VIM monoclonal antibody (Catalog # P00235) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VIM
规格: 100 ug/vial
价格: 299美元
至产商
微博分享
关注我们的微博
- 来邦网
- 英文来邦